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Establishment of genome analyses methods for non-model organisms

Planned Research

Project AreaGenetic bases for the evolution of complex adaptive traits
Project/Area Number 22128008
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKanazawa University

Principal Investigator

Nishiyama Tomoaki  金沢大学, 学際科学実験センター, 助教 (50390688)

Co-Investigator(Kenkyū-buntansha) SHIGENOBU Shuji  基礎生物学研究所, 生物機能解析センター, 特任准教授 (30399555)
KADOTA Koji  東京大学, 大学院農学生命科学研究科, 特任准教授 (60392221)
Co-Investigator(Renkei-kenkyūsha) KASAHARA Masahiro  東京大学, 新領域創成科学研究科, 講師 (60376605)
Research Collaborator YAMAGUCHI Katsushi  
Project Period (FY) 2010-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥110,240,000 (Direct Cost: ¥84,800,000、Indirect Cost: ¥25,440,000)
Fiscal Year 2014: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2013: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
Fiscal Year 2012: ¥22,620,000 (Direct Cost: ¥17,400,000、Indirect Cost: ¥5,220,000)
Fiscal Year 2011: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2010: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Keywordsアセンブリー / ゲノム / トランスクリプトーム / 遺伝子発現比較 / ゲノムアセンブリ / 遺伝学的地図 / RNA-seq / 計数比較 / ゲノムアセンブリー / 単分子シーケンサー / トランスクリプトーム解析 / 発現定量 / 一塩基多 / ヒメツリガネゴケ / アブラムシ / 遺伝子発現変動
Outline of Final Research Achievements

Genome and transcriptome sequencing, assembly, and annotation methods and expression profile analysis methods were developed to facilitate identification of genes responsible for complex adaptive traits in non-model organisms. Long mate-pair library construction enabled over Mb scaffolds and assembly utilizing long reads with single molecule real-time sequencing technology by Pacificbiosciences enabled over 100-kb contigs in multiple species. A novel robust normalization method for digital gene expression analyses were implemented and distributed in the R package TCC.

Report

(6 results)
  • 2015 Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (53 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (30 results) (of which Peer Reviewed: 30 results,  Open Access: 16 results,  Acknowledgement Compliant: 6 results) Presentation (18 results) (of which Invited: 4 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Cockroach GABA-B receptor subtypes: Molecular characterization, pharmacological properties and tissue distribution.2015

    • Author(s)
      Blankenburg S, Balfanz S, Hayashi Y, Shigenobu S, Miura T, Baumann O, Baumann A, Blenau W
    • Journal Title

      Neuropharmacology

      Volume: 88 Pages: 134-144

    • DOI

      10.1016/j.neuropharm.2014.08.022

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The evolutionary history of termites as inferred from 66 mitochondrial genomes.2015

    • Author(s)
      Bourguignon T, Lo N, Cameron SL, Sobotnik J, Hayashi Y, Shigenobu S, Watanabe D, Roisin Y, Miura T, Evans TA
    • Journal Title

      Mol Biol Evol

      Volume: 32 Issue: 2 Pages: 406-421

    • DOI

      10.1093/molbev/msu308

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A plant U-box protein, PUB4, regulates asymmetric cell division and cell proliferation in the root meristem.2015

    • Author(s)
      Kinoshita, A, Hove, C.A., Tabata, R., Yamada, M., Shimizu, N., Ishida, T., Yamaguchi K., Shigenobu, S., Takebayashi, Y., Iuchi, S., Kobayashi, M., Kurata, T., Wada, T., Seo, M., Hasebe, M., Bilou, I., Fukuda, H., Scheres, B., Heidstra, R., Sawa, S.
    • Journal Title

      Development

      Volume: 142 Issue: 3 Pages: 444-453

    • DOI

      10.1242/dev.113167

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mutations in plastidial 5-aminolevulinic acid biosynthesis genes suppress pleiotropic defect in shoot development of mitochondrial GABA shunt mutant in Arabidopsis2015

    • Author(s)
      Toyokura, K., Yamaguchi, K., Shigenobu, S., Fukaki, H., Tatematsu, K., and Okada, K.
    • Journal Title

      Plant and Cell Physiology

      Volume: 56 Issue: 6 Pages: 1229-1238

    • DOI

      10.1093/pcp/pcv050

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NMDA receptor activation upstream of methyl farnesoate signaling for short day-induced male offspring production in the water flea, Daphnia pulex2015

    • Author(s)
      Kenji Toyota, Hitoshi Miyakawa, Katsushi Yamaguchi, Shuji Shigenobu, Yukiko Ogino, Norihisa Tatarazako, Shinichi Miyagawa, Taisen Iguchi
    • Journal Title

      BMC Genomics

      Volume: 16 Issue: 1 Pages: 186-186

    • DOI

      10.1186/s12864-015-1392-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production2015

    • Author(s)
      Tao Wu, Takehiro Kamiya, Hiroko Yumoto, Naoyuki Sotta, Yamaguchi Katsushi, Shuji Shigenobu, Yoshikatsu Matsubayashi and Toru Fujiwara
    • Journal Title

      Journal of experimental botany

      Volume: 66 Issue: 13 Pages: 3657-3667

    • DOI

      10.1093/jxb/erv105

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Performance comparison of second- and third-generation sequencers using a bacterial genome with two chromosomes.2014

    • Author(s)
      Miyamoto M, Motooka D, Gotoh K, Imai T, Yoshitake K, Goto N, Iida T, Yasunaga T, Horii T, Arakawa K, Kasahara M, Nakamura S.
    • Journal Title

      BMC Genomics

      Volume: 15 Issue: 1 Pages: 699-699

    • DOI

      10.1186/1471-2164-15-699

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methylome diversification through changes in DNA methyltransferase sequence specificity.2014

    • Author(s)
      Yoshikazu Furuta, Hiroe Namba-Fukuyo, Tomoko F. Shibata, Tomoaki Nishiyama, Shuji Shigenobu, Yutaka Suzuki, Sumio Sugano, Mitsuyasu Hasebe, Ichizo Kobayashi
    • Journal Title

      PLoS Genetics

      Volume: 10(4) Issue: 4 Pages: e1004272-e1004272

    • DOI

      10.1371/journal.pgen.1004272

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative genome sequencing reveals genomic signature of desiccation tolerance in the anhydrobiotic midge2014

    • Author(s)
      Gusev O. Okuda T, Kikawada T etal.,
    • Journal Title

      Nat. Commun

      Volume: 5 Issue: 1 Pages: 1-9

    • DOI

      10.1038/ncomms5784

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Development of a PCR method for the detection of Helicobacter suis in gastric biopsy specimens.2014

    • Author(s)
      Matsui H, Takahashi T, Yamagata-Murayama S, Uchiyama I, Yamaguchi K, Shigenobu S, Matsumoto T, Kawakubo M, Ota H, Osaki T, Kamiya S, Takahashi S, Nakamura S, Nakamura M
    • Journal Title

      Helicobacter

      Volume: in press Issue: 4 Pages: 260-271

    • DOI

      10.1111/hel.12127

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss Physcomitrella patens.2014

    • Author(s)
      Keiko Sakakibara, Pascal Reisewitz, Tsuyoshi Aoyama, Thomas Friedrich, Sayuri Ando, Yoshikatsu Sato, Yosuke Tamada, Tomoaki Nishiyama, Yuji Hiwatashi, Tetsuya Kurata, Masaki Ishikawa, Hironori Deguchi, Stefan A. Rensing, Wolfgang Werr, Takashi Murata, Mitsuyasu Hasebe, Thomas Laux
    • Journal Title

      Development

      Volume: 141 Issue: 8 Pages: 1660-1670

    • DOI

      10.1242/dev.097444

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Whole-genome sequence of Burkholderia sp. strain RPE67, a bacterial gut symbiont of the bean bug Riptortus pedestris2014

    • Author(s)
      Takeshita K., Shibata T. F., Nikoh N., Yamaguchi K., Nishiyama T., Hasebe M., Fukatsu T., Shigenobu S., Kikuchi Y.
    • Journal Title

      Genome Announcements

      Volume: 2(3) Issue: 3

    • DOI

      10.1128/genomea.00556-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification and Characterization of an Arabidopsis Mutant with Altered Localization of NIP5;1, a Plasma Membrane Boric Acid Channel, Reveals the Requirement for D-Galactose in Endomembrane Organization.2014

    • Author(s)
      Uehara, M., Wang, S., Kamiya, T., Shigenobu, S., Yamaguchi, K., Fujiwara, T., Naito, S., and Takano, J.
    • Journal Title

      Plant Cell Physiology

      Volume: 55 Issue: 4 Pages: 704-714

    • DOI

      10.1093/pcp/pct191

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sex chromosome turnover contributes to genomic divergence between incipient stickleback species.2014

    • Author(s)
      Yoshida, K., Makino, T., Yamaguchi, K., Shigenobu, S., Hasebe, M., Kawata, M., Kume, M., Mori, S., Peichel, C.L., Toyoda, A., et al.
    • Journal Title

      PLoS genetics

      Volume: 10 Issue: 3 Pages: e1004223-e1004223

    • DOI

      10.1371/journal.pgen.1004223

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of gene expression of Paramecium bursaria with and without Chlorella variabilis symbionts2014

    • Author(s)
      Kodama, Y., Suzuki, H., Dohra, H., Sugii, M., Kitazume, T., Yamaguchi, K., Shigenobu, S., and Fujishima, M.
    • Journal Title

      BMC Genomics

      Volume: 15 Issue: 1 Pages: 183-183

    • DOI

      10.1186/1471-2164-15-183

    • NAID

      120005611450

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] TCC: an R package for comparing tag count data with robust normalization strategies.2013

    • Author(s)
      Sun, J., Nishiyama, T., Shimizu, K., and Kadota, K.
    • Journal Title

      BMC Bioinformatics

      Volume: 14 Issue: 1 Pages: 219-219

    • DOI

      10.1186/1471-2105-14-219

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Complete genome sequence of Burkholderia sp. strain RPE64, bacterial symbiont of the bean bug Riptortus pedestris2013

    • Author(s)
      Shibata T., Maeda T., Nikoh N., Yamaguchi K., Oshima K., Hattori M., Nishiyama T., Hasebe M., Fukatsu T., Kikuchi Y., Shigenobu S.
    • Journal Title

      Genome Announcements

      Volume: 1(4) Issue: 4

    • DOI

      10.1128/genomea.00441-13

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Too much love, a novel Kelch repeat-containing F-box protein, functions in the long-distance regulation of the legume-Rhizobium symbiosis2013

    • Author(s)
      Takahara, M., Magori, S., Soyano, T., Okamoto, S., Yoshida, C., Yano, K., Sato, S., Tabata, S., Yamaguchi, K., Shigenobu, S., et al
    • Journal Title

      Plant Cell Physiol

      Volume: 54 Issue: 4 Pages: 433-447

    • DOI

      10.1093/pcp/pct022

    • NAID

      10031163838

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The draft genomes of soft-shell turtle and green sea turtle yield insights into the development and evolution of the turtle-specific body plan.2013

    • Author(s)
      Wang, Z., Pascual-Anaya, J., Zadissa, A., Li, W., Niimura, Y., Huang, Z., Li, C., White, S., Xiong, Z., Fang, D., et al.
    • Journal Title

      Nature genetics

      Volume: 45 Issue: 6 Pages: 701-706

    • DOI

      10.1038/ng.2615

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Construction and characterization of normalized cDNA libraries by 454 pyrosequencing and estimation of DNA methylation levels in three distantly related termite specie2013

    • Author(s)
      Hayashi Y, Shigenobu S, Watanabe D, Toga K, Saiki R, Shimada K, Bourguignon T, Lo N, Hojo M, Maekawa K, Miura T
    • Journal Title

      PLOS ONE

      Volume: 8 Issue: 9 Pages: e76678-e76678

    • DOI

      10.1371/journal.pone.0076678

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Aphids evolved novel secreted proteins for symbiosis with bacterial endosymbiont2013

    • Author(s)
      Shigenobu, S. and Stern, D
    • Journal Title

      Proc. Royal Soc. B

      Volume: 280 Issue: 1750 Pages: 20121952-20121952

    • DOI

      10.1098/rspb.2012.1952

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A normalization strategy for comparing tag count data2012

    • Author(s)
      Kadota K., Nishiyama T., Shimizu K.
    • Journal Title

      Algorithms Mol. Biol.

      Volume: 7 Issue: 1 Pages: 5-5

    • DOI

      10.1186/1748-7188-7-5

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.2012

    • Author(s)
      Nishiyama T., Miyawaki K., Ohshima M., Thompson K., Nagashima A., Hasebe M., Kurata T
    • Journal Title

      PLoS One

      Volume: 7 Issue: 5 Pages: e36471-e36471

    • DOI

      10.1371/journal.pone.0036471

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exploration and characterization of genes involved in the synthesis of diterpene defence secretion in nasute termite soldiers.2012

    • Author(s)
      Hojo M, Maekawa K, Saitoh S, Shigenobu S, Miura T, Hayashi Y, Tokuda G, Maekawa H
    • Journal Title

      Insect Mol Biol

      Volume: 21 Issue: 6 Pages: 545-557

    • DOI

      10.1111/j.1365-2583.2012.01162.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of an EMS-induced causal mutation in a gene required for boron-mediated root development by low-coverage genome re-sequencing in Arabidopsis.2012

    • Author(s)
      Ryo Tabata, Takehiro Kamiya, Shuji Shigenobu, Katsushi Yamaguchi, Masashi Yamada, Mitsuyasu Hasebe, Toru Fujiwara, Shinichiro Sawa
    • Journal Title

      Plant Signaling & Behavior

      Volume: 8 Issue: 1 Pages: e22534-e22534

    • DOI

      10.4161/psb.22534

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Selaginella genome identifies genetic changes associated with the evolution of vascular plants2011

    • Author(s)
      Banks, et al
    • Journal Title

      Science

      Volume: 332 Issue: 6032 Pages: 960-963

    • DOI

      10.1126/science.1203810

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluating methods for ranking differentially expressed genes applied to MicroArray Quality Control data2011

    • Author(s)
      Kadota K and Shimizu K
    • Journal Title

      BMC Bioinformatics

      Volume: 12 Issue: 1 Pages: 227-227

    • DOI

      10.1186/1471-2105-12-227

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomic revelations of a mutualism: the pea aphid and its obligate bacterial symbiont2011

    • Author(s)
      Shigenobu, S. and Wilson, A. C.
    • Journal Title

      Cellular and Molecular Life Sciences

      Volume: 68 Issue: 8 Pages: 1297-1297

    • DOI

      10.1007/s00018-011-0645-2

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Physcomitrella cyclin-dependent kinase A links cell cycle reactivation to other cellular changes during reprogramming of leaf cells2011

    • Author(s)
      Ishikawa, M., Murata, T., Sato, Y., Nishiyama, T., Hiwatashi, Y., Imai, A., Kimura, M., Sugimoto, N., Akita, A., Oguri, Y. et al.
    • Journal Title

      Plant Cell

      Volume: 23 Issue: 8 Pages: 2924-2938

    • DOI

      10.1105/tpc.111.088005

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome expansion and differential expression of amino acid transporters at the aphid/Buchnera symbiotic interface2011

    • Author(s)
      Price, D. R., Duncan, R. P., Shigenobu, S. and *Wilson, A. C.
    • Journal Title

      Molecular Biology and Evolution

      Volume: 28 Issue: 11 Pages: 3113-3126

    • DOI

      10.1093/molbev/msr140

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 様々な実験デザインに対応した頑健なRNA-seq発現変動解析パイプラインTCCの開発2013

    • Author(s)
      孫建強, 西山智明, 清水謙多郎, 門田幸二
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2013-12-03 – 2013-12-06
    • Related Report
      2013 Annual Research Report
  • [Presentation] ゲノムに刻まれた食虫植物の進化2013

    • Author(s)
      (12)福島健児, Likai Mao, 今井飛将, 西山智明, 野澤昌文, 住川直美, 笠原雅弘, 柴田朋子, 重信秀治, Xiaodong Fang, 長谷部光泰
    • Organizer
      NGS現場の会第三回研究会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2013-09-04 – 2013-09-05
    • Related Report
      2013 Annual Research Report
  • [Presentation] 第二世代シークエンサーによる高密度の遺伝学的地図作成法 ヒメツリガネゴケへの適用例2013

    • Author(s)
      鎌田寛彬, 笠原雅弘, 長谷部光泰, 西山智明
    • Organizer
      NGS現場の会第三回研究会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2013-09-04 – 2013-09-05
    • Related Report
      2013 Annual Research Report
  • [Presentation] 1分子シークエンサーを用いた非モデル生物の de novo ゲノム解析の現状2013

    • Author(s)
      柴田朋子, 西山智明, 重信秀治, 長谷部光泰
    • Organizer
      NGS現場の会第三回研究会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2013-09-04 – 2013-09-05
    • Related Report
      2013 Annual Research Report
  • [Presentation] TCC: 頑健な正規化法を実装した発現変動解析用 R パッケージ2013

    • Author(s)
      孫建強, 西山智明, 清水謙多郎, 門田幸二
    • Organizer
      NGS現場の会第三回研究会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2013-09-04 – 2013-09-05
    • Related Report
      2013 Annual Research Report
  • [Presentation] Optimization of Procedures of Genomic DNA Preparation in Diverse Organisms for Single Molecule Real-Time Sequencing.2013

    • Author(s)
      Tomoko Shibata, Tomoaki Nishiyama, Shuji Shigenobu, Mitsuyasu Hasebe
    • Organizer
      The 14th annual Advances in Genome Biology and Technology (AGBT) meeting
    • Place of Presentation
      フロリダ (Marco Island Marriott Resort & Golf Club))
    • Related Report
      2012 Annual Research Report
  • [Presentation] Rでトランスクリプトーム解析2013

    • Author(s)
      門田幸二
    • Organizer
      HPCI チュートリアルセミナー
    • Place of Presentation
      東京 (産総研臨海副都心センター別館8階)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 食品機能解析研究とバイオインフォマティクス2013

    • Author(s)
      門田幸二
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台 (東北大学川内北キャンパス)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Digital gene expression profiling by 5’-end sequencing of cDNAs during2012

    • Author(s)
      Tomoaki Nishiyama, Kaori Miyawaki, Masumi Ohshima, Kari Thompson, Akitomo Nagashima, Mitsuyasu Hasebe & Tetsuya Kurata
    • Organizer
      moss2012
    • Place of Presentation
      ニューヨーク (ニューヨーク植物園)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 1分子シークエンシングを用いた共生生物ゲノムのde novo アセンブリ2012

    • Author(s)
      柴田朋子、前田太郎、西山智明、菊池義智、重信修治、深津武馬、長谷部光泰
    • Organizer
      日本進化学会第14回東京大会
    • Place of Presentation
      東京 (首都大学東京)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Closterium and Chara genome and transcriptome analyses2012

    • Author(s)
      Jun Abe, Hidetoshi Sakayama, Yutaka Suzuki, Atsushi Toyoda, Yuki Tsuchikane, Hiroyuki Sekimoto and Tomoaki Nishiyama
    • Organizer
      Plant and Animal Genomes XX
    • Place of Presentation
      Towns & Country, San Diego (USA)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 次世代シーケンサーデータのde novo アセンブリー解析2012

    • Author(s)
      西山智明,阿部淳,土金勇樹,坂山英俊,関本弘之
    • Organizer
      第53回植物生理学会年会
    • Place of Presentation
      京都産業大, 京都
    • Related Report
      2011 Annual Research Report
  • [Presentation] Next Generation Sequencing Technologies for Identification of Molecular Basis of Complex Adaptive Traits2011

    • Author(s)
      Tomoaki Nishiyama
    • Organizer
      The 20th CDB Meeting : Molecular Bases for Evolution of Complex Traits
    • Place of Presentation
      理研神戸研究所(兵庫県)(招待講演)
    • Year and Date
      2011-02-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Land Plant Evolution Learnt from Moss and Lycophyte Genomes2011

    • Author(s)
      Tomoaki Nishiyama
    • Organizer
      SMBE2011
    • Place of Presentation
      Kyoto Univ, Kyoto
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] RNAseqによる定量的解析とqPCR、マイクロアレイなどとの比較2010

    • Author(s)
      門田幸二
    • Organizer
      新学術領域研究「複合適応形質進化の遺伝子基盤解明」・複合適応形質進化インフォマティクスオープンセミナー
    • Place of Presentation
      金沢大学(石川県)
    • Year and Date
      2010-12-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Genomic revelations of a mutualism : the pea aphid and its obligate bacterial symbiont2010

    • Author(s)
      Shuji Shigenobu
    • Organizer
      Memorial Symposium for the 26th International Prize for Biology "Biology of Symbiosis"-Celebrating Dr.Nancy Moran
    • Place of Presentation
      つくば国際会議場(茨城県)(招待講演)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 非モデル生物のゲノム解読と責任遺伝子同定の展望2010

    • Author(s)
      西山智明
    • Organizer
      公開シンポジウム「複合適応形質の進化」
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] トランスクリプトーム解析の一手段としての次世代シーケンサーデータ解析~実演を中心に~2010

    • Author(s)
      門田幸二
    • Organizer
      日本バイオインフォマティクス学会・第2回アグリバイオインフォマティクス研究会
    • Place of Presentation
      琉球大学(沖縄県)
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Book] トランスクリプトーム解析2014

    • Author(s)
      門田幸二
    • Total Pages
      240
    • Publisher
      共立出版
    • Related Report
      2014 Annual Research Report
  • [Book] 「非モデル植物におけるNGSを用いたゲノム統合解析」(菅野純夫、鈴木穣 監『次世代シーケンサー』)2012

    • Author(s)
      西山智明、長谷部光泰
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2012 Annual Research Report
  • [Remarks] HOWTO

    • URL

      http://koke.asrc.kanazawa-u.ac.jp/HOWTO/

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
  • [Remarks] (Rで)塩基配列解析

    • URL

      http://www.iu.a.u-tokyo.ac.jp/~kadota/r_seq.html

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
  • [Remarks] (Rで)塩基配列解析

    • URL

      http://www.iu.a.u-tokyo.ac.jp/~kadota/r_seq.html

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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