| Budget Amount *help |
¥124,020,000 (Direct Cost: ¥95,400,000、Indirect Cost: ¥28,620,000)
Fiscal Year 2014: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2013: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2012: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2010: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
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| Outline of Final Research Achievements |
Eukaryotic genome is regulated as chromatin structure whose components are mainly DNA and histones. To understand molecular mechanisms behind a variety of DNA-mediated reactions, it is important to investigate missing roles of histones. In this study, we found novel roles of histones in a variety of DNA-mediated reactions including transcription, DNA replication, DNA repair, and chromosome segregation, as follows. 1) A novel role of histone surface in transcription elongation coupled H3-K36 methylation is found, 2) Protection of Sgo1 via histone surfaces is critical for faithful chromosome segregation, 3) Histone H2B is a common subunit for histone H2A-H2B dimer and histone variant H2A.Z-H2B dimer. Although D71 residue of H2B physically interacts with H2A and H2A.Z in corresponding dimers, H2B-D71A mutation mainly affect the function of H2A.Z-H2B rather than H2A-H2B, 4) Histone chaperone FACT may destabilize histones in ahead of replication fork and support fork progression.
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