| Budget Amount *help |
¥129,870,000 (Direct Cost: ¥99,900,000、Indirect Cost: ¥29,970,000)
Fiscal Year 2014: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2013: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2012: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2010: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
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| Outline of Final Research Achievements |
We examined molecular mechanism of transcription-coupled nucleotide excision repair (TC-NER) that quickly removes transcription-blocking DNA damage on the transcribed strands, and leads to resumption of transcription after DNA damage. We found that (1) Ubiquitylation of the largest subunit of RNA polymerase II by the CSA complex is indispensable for TC-NER. (2) Novel TC-NER factor, UVSSA-USP7 complex, is required for protection of CSB from proteasome-mediated degradation after UV-irradiation, leading to resumption of transcription. (3) We revealed novel functions of NER factors, XPG and XPD. XPG is required for transcription elongation of primary response gene FOS and house keeping gene EEF1A1, and XPD is included in the novel protein complex, named as MMXD, which is involved in chromosome segregation.
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