| Budget Amount *help |
¥127,920,000 (Direct Cost: ¥98,400,000、Indirect Cost: ¥29,520,000)
Fiscal Year 2014: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2013: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
Fiscal Year 2012: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2010: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
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| Outline of Final Research Achievements |
In this study, we have assessed the biological effects and repair mechanism of DNA-protein cross-link (DPC) damage. DPCs inhibited the translocation of replicative helicases, showing a unique mechanism of replisome stalling. DPCs caused backup of RNA polymerases, resulting in error-prone transcription. We have established a novel method of DPC detection using fluorescence labeling, and analyzed the induction of genomic DPCs by aldehydes, ionizing radiation, and anticancer drugs and their repair. We also showed that knockdown of RUVBL2, an essential subunit of a chromatin remodeling complex INO80, conferred a moderate sensitivity to DPC-inducing agents, suggesting a role of the chromatin remodeling complex in homologous recombination.
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