Identification of self-derived molecules recognized by immune sensors

• Project Area: Reevaluation of self recognition by immune system to decipher its physiological advantages and pathological risk
• Project/Area Number: 22H05183
• Research Category: Grant-in-Aid for Transformative Research Areas (A)
• Allocation Type: Single-year Grants
• Review Section: Transformative Research Areas, Section (III)
• Research Institution: The University of Osaka
• Principal Investigator: 山崎 晶 大阪大学, 微生物病研究所, 教授 (40312946)
• Co-Investigator(Kenkyū-buntansha): 長江 雅倫 東北医科薬科大学, 薬学部, 講師 (60619873) ; 井貫 晋輔 徳島大学, 大学院医歯薬学研究部(薬学域), 教授 (70736272)
• Project Period (FY): 2022-06-16 – 2027-03-31
• Project Status: Granted (Fiscal Year 2026)
• Budget Amount: ¥181,610,000 (Direct Cost: ¥139,700,000、Indirect Cost: ¥41,910,000); Fiscal Year 2026: ¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000); Fiscal Year 2025: ¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000); Fiscal Year 2024: ¥37,180,000 (Direct Cost: ¥28,600,000、Indirect Cost: ¥8,580,000); Fiscal Year 2023: ¥38,480,000 (Direct Cost: ¥29,600,000、Indirect Cost: ¥8,880,000); Fiscal Year 2022: ¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
• Keywords: 免疫学 / 自己認識 / 自然免疫受容体 / Dectin-1 / 自然免疫型T細胞 / T細胞受容体 / 免疫センサー / 自己分子 / C型レクチン受容体

Outline of Research at the Start

「多種多様な物質の識別」は免疫系が獲得した最も特徴的な能力である。この能力は、生物 が様々な外敵を感知するために進化してきたと考えられているが、近年、自己成分の認識にも関与していることがわかってきた。我々はこれまで、免疫受容体が認識する分子の同定プラットフォームを構築し、複数の自己リガンドを発見してきた。本計画研究では、この方法論を領域内で共有し、免疫センサーが認識する自己成分の戦略的同定を目指す。また、新たに同定した自己相互作用がもたらす生体応答の功罪 を明らかにすることで「免疫受容体による自己認識」が担う高解像度な自己監視機構の実態と免疫学を超えた普遍的な生物学的意義の解明を目的とする。

Outline of Annual Research Achievements

本研究では、生物有機化学的アプローチによって免疫センサーが認識する自己成分の同定・機能解析に取り組んでいる。当該年度は、前年度に引き続き（１）自然免疫型T細胞の制御に関わる自己由来脂質の構造展開、（２）可視光応答性光触媒を用いた自己成分同定手法の開発基盤検討を行った。
（１）自然免疫型T細胞の制御に関わる自己脂質の構造展開
山崎らによって同定された自己由来の脂質関連化合物の機能解析研究のために、新たな誘導体を設計合成した。
（２）可視光応答性光触媒を用いた自己成分同定手法の開発基盤検討
前年度までに開発した可視光応答性光触媒反応について、細胞上で反応を行うための条件を確立した。
本研究の学術的問いは、免疫受容体による自己認識が免疫系を超えてどのように生体に有益な応答をもたらすのか、また、その制御の破綻はどのようなリスクがあるのか、というものである。この問いに対して、免疫受容体の自己認識に関する進化的な研究はこれまでなされていなかった。これまでの免疫受容体に関する研究は主に哺乳類に限定されており、免疫受容体の進化的祖先が自己リガンドを認識するのか非自己リガンドを認識するのかが一切不明であった。そこで令和6年度は、自然免疫受容体の一種であるレクチン受容体に焦点を当て、ゲノムデータベースから進化的な起源となる遺伝子を探索・同定した。さらに、ゲノム解析で得られたレクチン受容体のリガンド認識に関する構造生物学的研究を行った。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

自然免疫型T細胞の制御に関わる自己由来脂質の機能解析研究は順調に進んでいる。前年度に引き続き、機能解析のための新たな誘導体を設計し、合成ルートを確立した。
可視光応答性光触媒を用いた自己成分同定手法の開発検討についても、細胞上で反応を行うための検討を行い、条件を確立した。また、質量分析によって、タンパク質中のアスパラギン酸やグルタミン酸側鎖のカルボキシ基と特異的に化学修飾反応が進行することを確認した。さらに、光触媒からのエネルギー移動機構により化学修飾反応が進行することを確認した。
レクチン受容体遺伝子の脊椎動物における進化的保存性は不明であった。そこでヒトMincleに相同な遺伝子をデータベースから探索した所、硬骨魚類にも相同遺伝子が存在することを見出した。また、Mincleのアダプター分子であるFcRγ遺伝子も魚類で保存されていることを明らかにした。そこで魚類Mincleと魚類FcRγを培養細胞に共発現した所、協同的な発現の上昇を確認した。さらに魚類Mincleのリガンド結合ドメインの立体構造をX線結晶構造解析によって明らかにした所、魚類Mincleはリガンド結合ポケットが狭く、内因性糖脂質で見られる単糖は結合できるが、外因性糖脂質に見られる二糖以上の糖鎖は結合できないことが示唆された。

Strategy for Future Research Activity

今後も、引き続き、自然免疫型T細胞の制御に関わる脂質関連化合物の機能解析、自己成分同定手法の開発基盤検討を継続する。
（１）自然免疫型T細胞の制御に関わる脂質リガンドの構造展開、機能解析
自然免疫型T細胞の制御に関わる脂質リガンドの構造展開を進め、ケミカルプローブや創薬シーズとなる機能性脂質への展開を目指す。
（２）可視光応答性光触媒を用いた自己成分同定手法の開発基盤検討
化学修飾のさらなる効率化を図るため、修飾タグや触媒の改変を検討する。前年度に引き続きモデル細胞等を用いて、細胞表面分子の修飾検討を実施する。
これまでの研究から、レクチン受容体の祖先型遺伝子は自己認識に特化している可能性が示唆され、免疫受容体における自己認識機構が非自己の認識よりも普遍的な現象である可能性が考えられた。これは、免疫受容体は内因性分子の攪乱をモニターすることが普遍的な生理機能であることを示唆しており、自己認識機構を解明することの重要性を裏付けるものである。今後は、研究の対象を脊椎動物に拡大し、それぞれのリガンド認識特異性を明らかにすることで、外因性リガンドに対する認識能を獲得するに至った過程を調べる予定である。この研究によって、どのような選択圧がレクチン受容体の非自己の認識能獲得に至らしめたのかを明らかにしたい。

Research Products

• [Journal Article] Longitudinal analysis of immune responses to SARS-CoV-2 recombinant vaccine S-268019-b in phase 1/2 prime-boost study (2025)
  • Author(s): Fujitani Masaya、Lu Xiuyuan、Shinnakasu Ryo、Inoue Takeshi、Kidani Yujiro、Seki Naomi M. and others
  • Journal Title: Frontiers in Immunology Volume: 16
  • DOI: 10.3389/fimmu.2025.1550279
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of α-galactosylceramide as an endogenous mammalian antigen for iNKT cells (2024)
  • Author(s): Hosono Yuki、Tomiyasu Noriyuki、Kasai Hayato、Ishikawa Eri、Takahashi Masatomo、Imamura Akihiro、Ishida Hideharu、Compostella Federica、Kida Hiroshi、Kumanogoh Atsushi、Bamba Takeshi、Izumi Yoshihiro、Yamasaki Sho
  • Journal Title: Journal of Experimental Medicine Volume: 222 Issue: 2
  • DOI: 10.1084/jem.20240728
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A conserved human CD4+ T cell subset recognizing the mycobacterial adjuvant trehalose monomycolate (2024)
  • Author(s): Sakai Yuki、Asa Minori、Hirose Mika、Kusuhara Wakana、Fujiwara Nagatoshi、Tamashima Hiroto、Ikazaki Takahiro and others
  • Journal Title: Journal of Clinical Investigation Volume: 135 Issue: 6 Pages: 6-6
  • DOI: 10.1172/jci185443
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Analysis of antigen specificity of Treg clonotypes expanded upon SARS-CoV-2 infection (2024)
  • Author(s): Takeuchi Yukiko、Ishikawa Eri、Sato Takashi、Shinkai Masaharu、Takahashi Yoshimasa、Lu Xiuyuan、Yamasaki Sho
  • Journal Title: International Immunology Volume: 37 Issue: 5 Pages: 287-293
  • DOI: 10.1093/intimm/dxae072
  • Peer Reviewed
• [Journal Article] Development of Ribityllumazine Analogue as Mucosal-Associated Invariant T Cell Ligands (2024)
  • Author(s): Takasaki Ryosuke、Ito Emi、Nagae Masamichi、Takahashi Yuki、Matsuoka Takuro、Yasue Wakana、Arichi Norihito、Ohno Hiroaki、Yamasaki Sho、Inuki Shinsuke
  • Journal Title: Journal of the American Chemical Society Volume: 146 Issue: 43 Pages: 29964-29976
  • DOI: 10.1021/jacs.4c12997
  • Peer Reviewed
• [Journal Article] Regulation of MAIT cells through host-derived antigens (2024)
  • Author(s): Ito Emi、Yamasaki Sho
  • Journal Title: Frontiers in Immunology Volume: 15
  • DOI: 10.3389/fimmu.2024.1424987
  • Peer Reviewed / Open Access
• [Journal Article] Ectopic Expression of C-Type Lectin Mincle Renders Mice Susceptible to Staphylococcal Pneumonia (2024)
  • Author(s): Hollwedel Femke D、Maus Regina、Stolper Jennifer、Iwai Satoru、Kasai Hayato、Holtfreter Silva、Pich Andreas、Neubert Lavinia、Welte Tobias、Yamasaki Sho、Maus Ulrich A
  • Journal Title: The Journal of Infectious Diseases Volume: 230 Issue: 1 Pages: 198-208
  • DOI: 10.1093/infdis/jiad608
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Early acquisition of S-specific Tfh clonotypes after SARS-CoV-2 vaccination is associated with the longevity of anti-S antibodies (2024)
  • Author(s): Lu Xiuyuan、Hayashi Hiroki、Ishikawa Eri、Takeuchi Yukiko、Dychiao Julian Vincent Tabora、Nakagami Hironori、Yamasaki Sho
  • Journal Title: eLife Volume: 12
  • DOI: 10.7554/elife.89999
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Crystal structure of the complex of CLEC12A and an antibody that interferes with binding of diverse ligands (2024)
  • Author(s): Mori Shotaro、Nagae Masamichi、Yamasaki Sho
  • Journal Title: International Immunology Volume: 36 Issue: 6 Pages: 279-290
  • DOI: 10.1093/intimm/dxae006
  • Peer Reviewed
• [Journal Article] Chimeric NOD2 Mincle Agonists as Vaccine Adjuvants (2024)
  • Author(s): Dangerfield Emma M.、Ishizuka Shigenari、Kodar Kristel、Yamasaki Sho、Timmer Mattie S. M.、Stocker Bridget L.
  • Journal Title: Journal of Medicinal Chemistry Volume: 67 Issue: 7 Pages: 5373-5390
  • DOI: 10.1021/acs.jmedchem.3c01840
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Myeloid C-type lectin receptors in innate immune recognition (2024)
  • Author(s): Reis e Sousa Caetano、Yamasaki Sho、Brown Gordon D.
  • Journal Title: Immunity Volume: 57 Issue: 4 Pages: 700-717
  • DOI: 10.1016/j.immuni.2024.03.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sulfated bile acid is a host-derived ligand for MAIT cells. (2024)
  • Author(s): Ito E, Inuki S, Izumi Y, Takahashi M, Dambayashi Y, Ciacchi L, Awad W, Takeyama A, Shibata K, Mori S, Mak JYW, Fairlie DP, Bamba T, Ishikawa E, Nagae M, Rossjohn J, Yamasaki S*.
  • Journal Title: Science Immunology Volume: 9(91) Issue: 91 Pages: 100492-100492
  • DOI: 10.1126/sciimmunol.ade6924
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Linkage-Editing Pseudo-Glycans: A Reductive α-Fluorovinyl-<i>C</i>-Glycosylation Strategy to Create Glycan Analogs with Altered Biological Activities (2024)
  • Author(s): Moriyama Takahiro et. al.
  • Journal Title: Journal of the American Chemical Society Volume: 146 Issue: 3 Pages: 2237-2247
  • DOI: 10.1021/jacs.3c12581
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Self-recognition through Dectin-1 exacerbates liver inflammation (2024)
  • Author(s): Torigoe S, Lowman DW, Sugiki T, Williams DL, Yamasaki S
  • Journal Title: Genes Cells Volume: 29(4) Issue: 4 Pages: 316-327
  • DOI: 10.1111/gtc.13106
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Fungal chitin-binding glycoprotein induces Dectin-2-mediated allergic airway inflammation synergistically with chitin (2024)
  • Author(s): Muraosa Yasunori、Hino Yutaro、Takatsuka Shogo、Watanabe Akira、Sakaida Emiko、Saijo Shinobu、Miyazaki Yoshitsugu、Yamasaki Sho、Kamei Katsuhiko
  • Journal Title: PLOS Pathogens Volume: 20 Issue: 1 Pages: 1011878-1011878
  • DOI: 10.1371/journal.ppat.1011878
  • Peer Reviewed / Open Access
• [Journal Article] Single-cell analysis for identification of T-cell clonotypes associated with IgG4 production of autoimmune pancreatitis (2024)
  • Author(s): Shibata Kensuke、Fujimori Nao、Oono Takamasa、Motooka Daisuke、Okuzaki Daisuke、Sonoda Koh-Hei、Ogawa Yoshihiro、Yamasaki Sho
  • Journal Title: Gastroenterology Report Volume: 11
  • DOI: 10.1093/gastro/goad071
  • Peer Reviewed / Open Access
• [Journal Article] Intranasal immunization with an RBD-hemagglutinin fusion protein harnesses preexisting immunity to enhance antigen-specific responses (2023)
  • Author(s): Kawai Atsushi、Tokunoh Nagisa、Kawahara Eigo、Tamiya Shigeyuki、Okamura Shinya、Ono Chikako、Anindita Jessica、Tanaka Hiroki、Akita Hidetaka、Yamasaki Sho、Kunisawa Jun、Okamoto Toru、Matsuura Yoshiharu、Hirai Toshiro、Yoshioka Yasuo
  • Journal Title: Journal of Clinical Investigation Volume: 133 Issue: 23 Pages: 1-15
  • DOI: 10.1172/jci166827
  • Peer Reviewed
• [Journal Article] Establishment of an MR1 Presentation Reporter Screening System and Identification of Phenylpropanoid Derivatives as MR1 Ligands (2023)
  • Author(s): Matsuoka Takuro、Hattori Akira、Oishi Shinya、Araki Mitsugu、Ma Biao、Fujii Toshiki、Arichi Norihito、Okuno Yasushi、Kakeya Hideaki、Yamasaki Sho、Ohno Hiroaki、Inuki Shinsuke
  • Journal Title: Journal of Medicinal Chemistry Volume: 66 Issue: 17 Pages: 12520-12535
  • DOI: 10.1021/acs.jmedchem.3c01122
  • Peer Reviewed
• [Journal Article] Archaeal glycerolipids are recognized by C-type lectin receptor Mincle. (2023)
  • Author(s): Oka S, Watanabe M, Ito E, Takeyama A, Matsuoka T, Takahashi M, Izumi Y, Arichi N, Ohno H*, Yamasaki S*, Inuki S*.
  • Journal Title: Journal of the American Chemical Society Volume: 145(33) Issue: 33 Pages: 18538-18548
  • DOI: 10.1021/jacs.3c05473
  • Peer Reviewed
• [Journal Article] PGL-III, a Rare Intermediate of <i>Mycobacterium leprae</i> Phenolic Glycolipid Biosynthesis, Is a Potent Mincle Ligand (2023)
  • Author(s): Ishizuka Shigenari、van Dijk J. Hessel M.、Kawakita Tomomi、Miyamoto Yuji、Maeda Yumi、Goto Masamichi、Le Calvez Guillaume、Groot L. Melanie、Witte Martin D.、Minnaard Adriaan J.、van der Marel Gijsbert A.、Ato Manabu、Nagae Masamichi、Cod?e Jeroen D. C.、Yamasaki Sho
  • Journal Title: ACS Central Science Volume: 9 Issue: 7 Pages: 1388-1399
  • DOI: 10.1021/acscentsci.3c00040
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural basis for plastic glycolipid recognition of the C-type lectin Mincle (2023)
  • Author(s): Furukawa Atsushi、Shuchi Yusuke、Wang Jiaqi、Guillen-Poza Pablo Adrian、Ishizuka Shigenari、Kagoshima Misuzu、Ikeno Risa、Kumeta Hiroyuki、Yamasaki Sho、Matsumaru Takanori、Saitoh Takashi、Maenaka Katsumi
  • Journal Title: Structure Volume: 31 Issue: 9 Pages: 1077-1085
  • DOI: 10.1016/j.str.2023.05.018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Host-pathogen interactions in nontuberculous mycobacterial infections (2023)
  • Author(s): Lore NI, Yamasaki S, Simmonds RE, Jo EK
  • Journal Title: Front Immunol. Volume: 14 Pages: 1201159-1201159
  • DOI: 10.3389/fimmu.2023.1201159
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Aryl-functionalised α,α'-Trehalose 6,6'-Glycolipid Induces Mincle-independent Pyroptotic Cell Death (2023)
  • Author(s): Kodar K, Dangerfield EM, Foster AJ, Forsythe D, Ishizuka S, McConnell MJ, Yamasaki S, Timmer MSM, Stocker BL
  • Journal Title: Inflammation Volume: 46(4) Issue: 4 Pages: 1365-1380
  • DOI: 10.1007/s10753-023-01814-5
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] COVID-19 relapse associated with SARS-CoV-2 evasion from CD4+ T-cell recognition in an agammaglobulinemia patient. (2023)
  • Author(s): Morita R, Kubota-Koketsu R, Lu X, Sasaki T, Nakayama EE, Liu YC, Okuzaki D, Motooka D, Wing JB, Fujikawa Y, Ichida Y, Amo K, Goto T, Hara J, Shirano M, Yamasaki S, Shioda T.
  • Journal Title: iScience Volume: 26(5) Issue: 5 Pages: 106685-106685
  • DOI: 10.1016/j.isci.2023.106685
  • Peer Reviewed / Open Access
• [Journal Article] Ligand‐Controlled Stereoselective Synthesis and Biological Activity of 2‐Exomethylene Pseudo‐glycoconjugates: Discovery of Mincle‐Selective Ligands (2023)
  • Author(s): Ikazaki Takahiro、Ishikawa Eri、Tamashima Hiroto、Akiyama Hisako、Kimuro Yusuke、Yoritate Makoto、Matoba Hiroaki、Imamura Akihiro、Ishida Hideharu、Yamasaki Sho、Hirai Go
  • Journal Title: Angewandte Chemie International Edition Volume: e202302569 Issue: 22
  • DOI: 10.1002/anie.202302569
  • Peer Reviewed
• [Journal Article] 6-C-Linked trehalose glycolipids signal through Mincle and exhibit potent adjuvant activity (2023)
  • Author(s): Thathsaranie P. Manthrirathna M.A.、Kodar Kristel、Ishizuka Shigenari、Dangerfield Emma M.、Xiuyuan Lu、Yamasaki Sho、Stocker Bridget L.、S. M. Timmer Mattie
  • Journal Title: Bioorganic Chemistry Volume: 133 Pages: 106345-106345
  • DOI: 10.1016/j.bioorg.2023.106345
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Immunomodulatory Functions of Glycolipids from Pathogens (2023)
  • Author(s): Guenther Carla、Watanabe Miyuki、Yamasaki Sho
  • Journal Title: Methods Mol. Biol., Glycolipids Methods and Protocols Volume: 2613 Pages: 23-31
  • DOI: 10.1007/978-1-0716-2910-9_3
  • ISBN: 9781071629093, 9781071629109
• [Journal Article] The kinetics of signaling through the common FcRγ chain determine cytokine profiles in dendritic cells (2023)
  • Author(s): Watanabe Miyuki、Motooka Daisuke、Yamasaki Sho
  • Journal Title: Science Signaling Volume: 16 Issue: 775
  • DOI: 10.1126/scisignal.abn9909
  • Peer Reviewed
• [Journal Article] Dectin-1/IL-15 Pathway Affords Protection against Extrapulmonary Aspergillus fumigatus Infection by Regulating Natural Killer Cell Survival (2023)
  • Author(s): Yoshikawa F.S.Y.、Wakatsuki Maki、Yoshida Kosuke、Yabe Rikio、Torigoe Shota、Yamasaki Sho、Barber Glen N.、Saijo Shinobu
  • Journal Title: Journal of Innate Immunity Volume: 15 Issue: 1 Pages: 1-15
  • DOI: 10.1159/000527188
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Direct activation of microglia by β-glucosylceramide causes phagoptosis of neurons that exacerbates Gaucher disease. (2023)
  • Author(s): Shimizu T, Schutt C R, Izumi Y, Tomiyasu N, Omahdi Z, Kano K, Takamatsu H, Aoki J, Bamba T, Kumanogoh A, Takao M, Yamasaki S*.
  • Journal Title: Immunity Volume: 56 Issue: 2 Pages: 1-13
  • DOI: 10.1016/j.immuni.2023.01.008
  • Peer Reviewed / Open Access
• [Journal Article] SARS-CoV-2に対するT細胞免疫 (2023)
  • Author(s): 坂井由葵, 山﨑晶
  • Journal Title: 実験医学 Volume: 41 Pages: 521-526
• [Journal Article] Fungal β‐mannosyloxymannitol glycolipids and their analogues: synthesis and Mincle‐mediated signaling activity (2022)
  • Author(s): Matsumaru T.、Sakuratani K.、Yanaka S.、Kato K.、Yamasaki S.、Fujimoto Y.
  • Journal Title: European Journal of Organic Chemistry Volume: - Issue: 20
  • DOI: 10.1002/ejoc.202200109
  • Peer Reviewed
• [Journal Article] Synthetic vaccines targeting Mincle through conjugation of trehalose dibehenate (2022)
  • Author(s): Hanna Cameron C.、Maxwell Joshua W. C.、Ismanto Hendra S.、Ashhurst Anneliese S.、Artner Lukas M.、Rudrawar Santosh、Britton Warwick J.、Yamasaki Sho、Payne Richard J.
  • Journal Title: Chemical Communications Volume: 58 Issue: 49 Pages: 6890-6893
  • DOI: 10.1039/d2cc02100e
  • Peer Reviewed
• [Journal Article] The intracellular pathogen <i>Francisella tularensis</i> escapes from adaptive immunity by metabolic adaptation (2022)
  • Author(s): Shibata Kensuke、Shimizu Takashi、Nakahara Mashio、Ito Emi、Legoux Francois、Fujii Shotaro、Yamada Yuka、Furutani-Seiki Makoto、Lantz Olivier、Yamasaki Sho、Watarai Masahisa、Shirai Mutsunori
  • Journal Title: Life Science Alliance Volume: 5 Issue: 10 Pages: e202201441-e202201441
  • DOI: 10.26508/lsa.202201441
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Trehalose diesters containing a polar functional group-modified lipid moiety: Synthesis and evaluation of Mincle-mediated signaling activity (2022)
  • Author(s): Matsumaru Takanori、Sueyoshi Kodai、Okubo Kana、Fujii Shusuke、Sakuratani Kasumi、Saito Ryota、Ueki Kazunari、Yamasaki Sho、Fujimoto Yukari
  • Journal Title: Bioorganic &amp; Medicinal Chemistry Volume: 75 Pages: 117045-117045
  • DOI: 10.1016/j.bmc.2022.117045
  • Peer Reviewed
• [Journal Article] Symbiotic bacteria-dependent expansion of MR1-reactive T cells causes autoimmunity in the absence of Bcl11b (2022)
  • Author(s): Shibata Kensuke、Motozono Chihiro、Nagae Masamichi、Shimizu Takashi、Ishikawa Eri、Motooka Daisuke、Okuzaki Daisuke、Izumi Yoshihiro、Takahashi Masatomo、Fujimori Nao、Wing James B.、Hayano Takahide、Asai Yoshiyuki、Bamba Takeshi、Ogawa Yoshihiro、Furutani-Seiki Makoto、Shirai Mutsunori、Yamasaki Sho
  • Journal Title: Nature Communications Volume: 13 Issue: 1 Pages: 6948-6948
  • DOI: 10.1038/s41467-022-34802-8
  • Peer Reviewed / Open Access
• [Journal Article] 感染防御に寄与するT細胞の解析 (2022)
  • Author(s): 細野裕貴、山﨑晶
  • Journal Title: Precision Medicine 11月臨時増刊号 Volume: 5 Pages: 1-4
• [Journal Article] ゴーシェ病における神経細胞死誘導の新たな分子機構 (2022)
  • Author(s): 清水隆、山﨑晶
  • Journal Title: 医学のあゆみ Volume: 283 Pages: 380-384
• [Journal Article] Self-referential immune recognition through C-type lectin receptors (2022)
  • Author(s): Guenther Carla、Nagae Masamichi、Yamasaki Sho
  • Journal Title: Adv. Immunol. Volume: 156 Pages: 1-23
  • DOI: 10.1016/bs.ai.2022.09.001
  • ISBN: 9780323989473
  • Peer Reviewed
• [Journal Article] Lectin recruits pathogenic bugs (2022)
  • Author(s): Schutt Charles R.、Yamasaki Sho
  • Journal Title: Journal of Experimental Medicine Volume: 220 Issue: 1
  • DOI: 10.1084/jem.20221732
• [Journal Article] Memory B Cells and Memory T Cells Induced by SARS-CoV-2 Booster Vaccination or Infection Show Different Dynamics and Responsiveness to the Omicron Variant (2022)
  • Author(s): Mise-Omata, S., M. Ikeda, M. Takeshita, Y. Uwamino, M. Wakui, T. Arai, A. Yoshifuji, K. Murano, H. Siomi, K. Nakagawara, M. Ohyagi, M. Ando, N. Hasegawa, H. Saya, M. Murata, K. Fukunaga, H. Namkoong, X. Lu, S. Yamasaki, and A. Yoshimura.
  • Journal Title: J Immunol Volume: 209 Issue: 11 Pages: 2104-2113
  • DOI: 10.4049/jimmunol.2200525
  • Peer Reviewed / Open Access
• [Journal Article] Human Dectin-1 is O-glycosylated and serves as a ligand for C-type lectin receptor CLEC-2 (2022)
  • Author(s): Haji Shojiro、Ito Taiki、Guenther Carla、Nakano Miyako、Shimizu Takashi、Mori Daiki、Chiba Yasunori、Tanaka Masato、Mishra Sushil K、Willment Janet A、Brown Gordon D、Nagae Masamichi、Yamasaki Sho
  • Journal Title: eLife Volume: 11
  • DOI: 10.7554/elife.83037
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Water-soluble trehalose glycolipids show superior Mincle binding and signaling but impaired phagocytosis and IL-1β production (2022)
  • Author(s): Manthrirathna M. A. Thathsaranie P.、Dangerfield Emma M.、Ishizuka Shigenari、Woods Aodhamair、Luong Brenda S.、Yamasaki Sho、Timmer Mattie S. M.、Stocker Bridget L.
  • Journal Title: Frontiers in Molecular Biosciences Volume: 9 Pages: 1015210-1015210
  • DOI: 10.3389/fmolb.2022.1015210
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dysfunctional Sars-CoV-2-M protein-specific cytotoxic T lymphocytes in patients recovering from severe COVID-19 (2022)
  • Author(s): Ogura Hideki、Gohda Jin、Lu Xiuyuan、Yamamoto Mizuki、Takesue Yoshio、Son Aoi、Doi Sadayuki、Matsushita Kazuyuki、Isobe Fumitaka、Fukuda Yoshihiro、Huang Tai-Ping、Ueno Takamasa、Mambo Naomi、Murakami Hiromoto、Kawaguchi Yasushi、Inoue Jun-ichiro、Shirai Kunihiro、Yamasaki Sho、Hirata Jun-Ichi、Ishido Satoshi
  • Journal Title: Nature Communications Volume: 13 Issue: 1 Pages: 7063-7063
  • DOI: 10.1038/s41467-022-34655-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Host immune system targeting mycobacterial lipids (2025)
  • Author(s): 山﨑晶
  • Organizer: Cold Spring Harbor Asia meeting on Bacterial Infection & Host Defense
  • Int'l Joint Research / Invited
• [Presentation] Sensing self- and non-self metabolites via immune systems (2024)
  • Author(s): 山﨑晶
  • Organizer: Strasbourg-Osaka Interdisciplinary Symposium
  • Int'l Joint Research / Invited
• [Presentation] T cell response to host metabolites (2024)
  • Author(s): 山﨑晶
  • Organizer: International Conference, Korean Society for Molecular and Cellular Biology
  • Int'l Joint Research / Invited
• [Presentation] Immune responses to mycobacterial lipids (2024)
  • Author(s): 山﨑晶
  • Organizer: EMBO-EMBL Symposium
  • Int'l Joint Research / Invited
• [Presentation] Self-recognition through immune sensors for bacteria (2024)
  • Author(s): 山﨑晶
  • Organizer: Cold Spring Harbor Asia meeting on Bacterial Infection & Host Defense
  • Int'l Joint Research / Invited
• [Presentation] Recognition of host-derived metabolites through unconventional T cells (2024)
  • Author(s): 山﨑晶
  • Organizer: CD1-MR1
  • Int'l Joint Research / Invited
• [Presentation] Immunological and Biochemical Aspects of C-Type Lectin Receptors in Fungal Infections (2024)
  • Author(s): 山﨑晶
  • Organizer: 2024 Keystone Symposia on Fungal Pathogens
  • Int'l Joint Research / Invited
• [Presentation] Human T cell responses against infection (2024)
  • Author(s): 山﨑晶
  • Organizer: 第6回日中韓免疫シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] Regulation of MAIT cells by a host-derived antigen (2023)
  • Author(s): 山﨑晶
  • Organizer: 九州大学 生体防御医学研究所Hot Spring Harbor国際シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] Deciphering clonotypic responses of human T cells against infection (2023)
  • Author(s): 山﨑晶
  • Organizer: Cold Spring Harbor Asia
  • Int'l Joint Research / Invited
• [Presentation] Self-referential immune perception through C- type lectin receptors (2023)
  • Author(s): 山﨑晶
  • Organizer: The 6th International GRK Symposium
  • Int'l Joint Research / Invited
• [Presentation] Immune responses against infection(感染に対抗するからだのしくみ) (2023)
  • Author(s): 山﨑晶
  • Organizer: 京都大学農学部１００周年記念国際シンポジウム
  • Invited
• [Presentation] 疾患におけるヒトT細胞クロノタイプ解析 (2023)
  • Author(s): 山﨑晶
  • Organizer: 第72回日本アレルギー学会学術大会
  • Invited
• [Presentation] レクチン受容体を介する異物認識機構 (2023)
  • Author(s): 山﨑晶
  • Organizer: 第43回阿蘇シンポジウム
  • Invited
• [Presentation] Sensing damaged self via C-type lectin receptors (2023)
  • Author(s): 山﨑晶
  • Organizer: 日豪Cell Death meeting(Japan and Australia Meeting on Cell Death 2023)
  • Int'l Joint Research / Invited
• [Presentation] Innate and acquired immune responses against mycobacterial lipids (2023)
  • Author(s): 山﨑 晶
  • Organizer: EID会議/日米合同パネル会議 U.S.-Japan Cooperative Medical Sciences Program
  • Int'l Joint Research / Invited
• [Presentation] A transient metabolite in Mycobacterium leprae PGL-I biosynthesis pathway is a potent PAMP (2023)
  • Author(s): Shigenari Ishizuka, J. Hessel M. van Dijk, Tomomi Kawakita, Yuji Miyamoto, Yumi Maeda, Masamichi Goto, Manabu Ato, Masamichi Nagae, Jeroen D. C. Codee, Sho Yamasaki
  • Organizer: EID会議/日米合同パネル会議 U.S.-Japan Cooperative Medical Sciences Program
• [Presentation] 抗酸菌に対する自然免疫と獲得免疫の共闘 (2023)
  • Author(s): 山﨑 晶
  • Organizer: 第96回日本細菌学会総会
  • Invited
• [Presentation] Recognition of internal/external metabolites via CLR and unconventional TCR (2023)
  • Author(s): 山﨑 晶
  • Organizer: メルボルン大学大阪大学合同シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] 免疫受容体による病原体の識別と細胞応答決定機構 (2023)
  • Author(s): 山﨑 晶
  • Organizer: 「感染・免疫・がん・炎症」の拠点シンポジウム
  • Invited
• [Presentation] Novel antigen for mucosal-associated invariant T (MAIT) cells (2022)
  • Author(s): Emi Ito, Shinsuke Inuki, Yoshihiro Izumi, Masatomo Takahashi, Eri Ishikawa, Kensuke Shibata, Takeshi Bamba and Sho Yamasaki
  • Organizer: EMBO Workshop: CD1/MR1 restricted T lymphocytes
  • Int'l Joint Research
• [Presentation] Innate Immune Sensing of Metabolites Causes Neuroinflammatory Diseases (2022)
  • Author(s): 山﨑 晶
  • Organizer: JSICR/MMCB合同シンポジウム
  • Invited
• [Presentation] MAIT細胞の新規脂質抗原の探索 (2022)
  • Author(s): 伊東瑛美、井貫晋輔、和泉自泰、高橋政友、石川絵里、柴田健輔、馬場健史、山﨑晶
  • Organizer: 第64回日本脂質生化学会
• [Presentation] Helicobacter pyloriに対する免疫応答と胃炎誘導機構 (2022)
  • Author(s): 山﨑晶
  • Organizer: 第28回日本ヘリコバクター学会学術集会
  • Invited
• [Presentation] グルコシルセラミドが誘導するグリア細胞異常に伴う神経細胞死とゴージェ病病態への寄与 (2022)
  • Author(s): 清水隆、Charles Schuut、山﨑晶
  • Organizer: 第30回日本Cell Death学会学術集会
• [Presentation] メタボロミクス技術を活用した新たなT細胞抗原の探索 (2022)
  • Author(s): 伊東瑛美、山﨑 晶
  • Organizer: 第16回メタボロームシンポジウム
  • Invited
• [Presentation] 感染に対する生体防御応答 (2022)
  • Author(s): 山﨑 晶
  • Organizer: 第33回日本生体防御学会学術総会
  • Invited
• [Presentation] 糖質を介する免疫受容体による自己認識機構 (2022)
  • Author(s): 山﨑 晶
  • Organizer: 第41回日本糖質学会年会
  • Invited
• [Presentation] レクチン受容体による結晶性物質の認識機構に関する構造基盤の解明 (2022)
  • Author(s): 長江雅倫、森正太郎、山﨑晶
  • Organizer: 第41回日本糖質学会年会
• [Presentation] 感染に対する免疫応答:SARS-CoV-2特異的Tfhクローンの同定と治療への展開 (2022)
  • Author(s): 山﨑 晶
  • Organizer: 第10回Transplant Immunology Forum
  • Invited
• [Presentation] Host Defense (2022)
  • Author(s): 山﨑 晶
  • Organizer: 2022 UBC&OU Virtual Symposium on Infectious Diseases and Drug Development
  • Int'l Joint Research / Invited
• [Presentation] T cell responses against SARS-CoV-2 (2022)
  • Author(s): 山﨑 晶
  • Organizer: Web The 29th FAOBMB & 2022 CSBMB Conference
  • Int'l Joint Research / Invited
• [Presentation] Regulation of immune responses against infection (2022)
  • Author(s): 山﨑 晶
  • Organizer: BK4 2022 International Symposium on Frontiers in Biomedical Science
  • Int'l Joint Research / Invited
• [Presentation] Regulation of acquired immunity against SARS-CoV2 (2022)
  • Author(s): 山﨑 晶
  • Organizer: 日独免疫ワークショップ
  • Int'l Joint Research / Invited
• [Presentation] 糖鎖を介する免疫受容体による自己認識機構 (2022)
  • Author(s): 山﨑 晶
  • Organizer: JCGGシンポジウム(第 19 回糖鎖科学コンソーシアム)
  • Invited
• [Presentation] 病原体を排除するからだのしくみ (2022)
  • Author(s): 山﨑 晶
  • Organizer: SpringX 超学校 CiDER(大阪大学感染症総合教育研究拠点)×ナレッジキャピタル 正しく学ぶ! 感染症から「いのち」と「くらし」を守る 講座
  • Invited
• [Presentation] 感染における病原体脂質と免疫受容体の攻防 (2022)
  • Author(s): 山﨑 晶
  • Organizer: (公)微生物化学研究所講演会
  • Invited
• [Presentation] T cell responses against SARS-CoV-2 (2022)
  • Author(s): 山﨑 晶
  • Organizer: 第51回日本免疫学会学術集会
• [Presentation] Self-antigen for mucosal-associated invariant T (MAIT) cell (2022)
  • Author(s): Emi Ito, Ami Takeyama, Shotaro Mori, Kensuke Shibata, Eri Ishikawa, Masamichi Nagae and Sho Yamasaki
  • Organizer: 第51回日本免疫学会学術集会
• [Presentation] Identification of T cells responding to mycobacterial lipids (2022)
  • Author(s): Yuki Sakai, Xiuyuan Lu, Sho Yamasaki, Takashi Yoshiyama, Yoshihiko Hoshino
  • Organizer: 第51回日本免疫学会学術集会
• [Presentation] Characterization of self glycolipids presented by CD1d that activate NKT cells (2022)
  • Author(s): Yuki Hosono, Noriyuki Tomiyasu, Yoshihiro Izumi, Akihiro Imamura, Eri Ishikawa, Atsushi Kumanogoh, Hideharu Ishida, Takeshi Bamba, Sho Yamasaki
  • Organizer: 第51回日本免疫学会学術集会
• [Presentation] Structural studies of public TCR against SARS-CoV-2 peptide clarified the broad spectrum of the clonotype (2022)
  • Author(s): Masamichi Nagae, Shotaro Mori, Sho Yamasaki
  • Organizer: 第51回日本免疫学会学術集会
• [Presentation] Direct activation of microglia by β-glucosylceramide exacerbates Gaucher disease (2022)
  • Author(s): Takashi Shimizu, Atsushi Kumanogoh, Sho Yamasaki
  • Organizer: 第51回日本免疫学会学術集会
• [Presentation] Deciphering pathogen-specific T cell responses to a clonotype resolution (2022)
  • Author(s): Xiuyuan Lu, Yuki Hosono, Shigenari Ishizuka, Eri Ishikawa, Atsushi Kumanogoh, Yoshimasa Takahashi, Sho Yamasaki
  • Organizer: 第51回日本免疫学会学術集会
  • Invited
• [Presentation] Signal kinetics via common FcRγ chain generates distinct cellular responses by altering chromatin landscape (2022)
  • Author(s): Miyuki Watanabe, Sho Yamasaki
  • Organizer: 第51回日本免疫学会学術集会
• [Presentation] 免疫受容体を介する生理活性脂質の生体応答 (2022)
  • Author(s): 山﨑 晶
  • Organizer: 京都大学農学研究科 第12回公開シンポジウム
  • Invited
• [Patent(Industrial Property Rights)] 糖脂質誘導体を有効成分とするＴ細胞活性化剤 (2024)
  • Inventor(s): 山崎 晶/坂井 由葵
  • Industrial Property Rights Holder: 山崎 晶/坂井 由葵
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2024-004685
  • Filing Date: 2024
• [Patent(Industrial Property Rights)] ＭＡＩＴ細胞リガンドの同定のための細胞、および当該細胞を用いる ＭＡＩＴ細胞リガンドのスクリーニング方法 (2023)
  • Inventor(s): 山﨑 晶/伊東 瑛美
  • Industrial Property Rights Holder: 山﨑 晶/伊東 瑛美
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2023-186971
  • Filing Date: 2023