| Budget Amount *help |
¥65,130,000 (Direct Cost: ¥50,100,000、Indirect Cost: ¥15,030,000)
Fiscal Year 2015: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2014: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2013: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2012: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
To immobilize various type of bioactive natural products on Handa beads effectively, we established the method that utilize reliable esterification and click reaction with azide and acetylene. We successfully prepared various bioactive natural product-immobilized Handa beads. (bioactive natural products: erythromycin derivative, vitamin K2, salicylic acid and others)
Through affinity purification with the bioactive natural product-immobilized Handa beads and biochemical analyses of the specific binding proteins to the bioactive natural product, we identified target proteins (Bak for vitamin K2; ferrochelatase for salicylic acid) for the bioactive natural products and elucidated mechanisms of action of the bioactive natural products. Additionally, exceptional results were achieved by research collaboration.
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