Development of a bio-screening method using intestinal epithelial cells

• Project Area: Chemical Biology using bioactive natural products as specific ligands: identification of molecular targets and regulation of bioactivity
• Project/Area Number: 23102003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Okamoto Ryuichi 東京医科歯科大学, 再生医療研究センター, 教授 (50451935)
• Co-Investigator(Kenkyū-buntansha): Nakamura Tetsuya 東京医科歯科大学, 医歯学(薬)学総合研究科, 寄附講座教授 (70265809)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥43,290,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥9,990,000); Fiscal Year 2015: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000); Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000); Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: 腸管上皮 / 初代培養 / 天然物リガンド / 生体活性評価 / 活性評価 / 天然物リガント / 炎症性腸疾患 / Notchシグナル / 上皮幹細胞 / 粘膜再生 / 正常腸管上皮 / 細胞移植 / 組織再生 / 薬剤排泄 / 薬物動態解析 / ライブ・イメージング

Outline of Final Research Achievements

Based on our culture method for primary human intestinal cells, we developed methods by which we can visualize and trace stem cells by lentiviral infection, or visualize their daughter cells through genetic modification in mice. Also, we developed our quantitative measurement system to evaluate the fluid/anion transport by intestinal epithelial cells, in addition to the system dedicated to measure the function of P-glycoprotein. Finally, through our collaborative work with professor Ishibashi of A02 group, we established an activity measurement system for Notch-receptor mediated signaling.

Research Products

• [Journal Article] Role of epithelial cells in the pathogenesis and treatment of inflammatory bowel disease. (2016)
  • Author(s): Ryuichi Okamoto, Mamoru Watanabe
  • Journal Title: J. Gastroenterol Volume: 51 Issue: 1 Pages: 11-21
  • DOI: 10.1007/s00535-015-1098-4
  • Peer Reviewed / Open Access
• [Journal Article] Matrix Metalloproteinase-14 Mediates Formation of Bile Ducts and Hepatic Maturation of Fetal Hepatic Progenitor Cells. (2016)
  • Author(s): Otani S, Kakinuma S, Kamiya A, Goto F, Kaneko S, Miyoshi M, Tsunoda T, Asano Y, Kawai-Kitahata F, Nitta S, Nakata T, Okamoto R, Itsui Y, Nakagawa M, Azuma S, Asahina Y, Yamaguchi T, Koshikawa N, Seiki M, Nakauchi H, Watanabe M.
  • Journal Title: Biochem Biophys Res Commun Volume: 469 Issue: 4 Pages: 1062-1068
  • DOI: 10.1016/j.bbrc.2015.12.105
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reduced human α-defensin 6 in non-inflamed jejunal tissue of Crohn’s disease patients (2016)
  • Author(s): Hayashi R , Tsuchiya K, Okamoto R, Watanabe M.et al.
  • Journal Title: Inflammatory Bowel Diseases Volume: 22 Pages: 1119-1128
  • Peer Reviewed
• [Journal Article] Perspectives for Regenerative Medicine in the Treatment of Inflammatory Bowel Diseases. (2015)
  • Author(s): Ryuichi Okamoto, Mamoru Watanabe
  • Journal Title: Digestion Volume: 92 Issue: 2 Pages: 73-77
  • DOI: 10.1159/000438663
  • Peer Reviewed / Open Access
• [Journal Article] TNFAIP3 promotes survival of CD4 T cells by restricting MTOR and promoting autophagy. (2015)
  • Author(s): Matsuzawa Y, Oshima S, Takahara M, Maeyashiki C, Nemoto Y, Kobayashi M, Nibe Y, Nozaki K, Nagaishi T, Okamoto R, Tsuchiya K, Nakamura T, Averil Ma, Watanabe M
  • Journal Title: Autophagy Volume: 11 Issue: 7 Pages: 1052-1062
  • DOI: 10.1080/15548627.2015.1055439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Atonal homolog 1 protein stabilized by tumor necrosis factor α induces high malignant potential in colon cancer cell line. (2015)
  • Author(s): Keita Fukushima, Kiichiro Tsuchiya,Okamoto Ryuichi, Mamoru Watanabe,et al
  • Journal Title: Cancer Sci Volume: 106 Issue: 8 Pages: 1000-7
  • DOI: 10.1111/cas.12703
  • Peer Reviewed / Open Access
• [Journal Article] RIPK3 regulates p62-LC3 complex formation via the caspase-8-dependent cleavage of p62. (2014)
  • Author(s): Y. Matsuzawa , S. Oshima, Y. Nibe, M.Kobayashi, C. Maeyashiki, Y. Nemoto, T. Nagaishi, R. Okamoto R, K. Tsuchiya, T. Nakamura, M. Watanabe.
  • Journal Title: Biochem Biophys Res Commun Volume: 456(1) Issue: 1 Pages: 298-304
  • DOI: 10.1016/j.bbrc.2014.11.075
  • Peer Reviewed / Open Access
• [Journal Article] Fluorescent labelling of intestinal epithelial cells reveals independent long-lived intestinal stem cells in a crypt. (2014)
  • Author(s): N. Horita, K. Tsuchiya, R. Hayashi, K. Fukushima, S. Hibiya, M. Fukuda, Y. Kano, T. Mizutani, Y. Nemoto, S. Yui, R. Okamoto, T. Nakamura, M. Watanabe
  • Journal Title: Biochem Biophys Res Commun Volume: 454 Issue: 4 Pages: 493-499
  • DOI: 10.1016/j.bbrc.2014.10.091
  • Peer Reviewed / Open Access
• [Journal Article] Myosin light chain kinase expression induced via tumor necrosis factor receptor 2 signaling in the epithelial cells regulates the development of colitis-associated carcinogenesis. (2014)
  • Author(s): Suzuki M, Nagaishi T, Yamazaki M, Onizawa M, Watabe T, Sakamaki Y, Ichinose S, Totsuka M, Oshima S, Okamoto R, Shimonaka M, Yagita H, Nakamura T, Watanabe M.
  • Journal Title: PLoS One Volume: 9 Issue: 2 Pages: e88369-e88369
  • DOI: 10.1371/journal.pone.0088369
  • Peer Reviewed / Open Access
• [Journal Article] Distinct expression patterns of Notch ligands, Dll1 and Dll4, in normal and inflamed mice intestine. (2014)
  • Author(s): Shimizu H, Okamoto R, Ito G, Nakata T, Suzuki K, Murano T, Mizutani T, Tsuchiya K, Nakamura T, Hozumi K, Watanabe M.
  • Journal Title: PeerJ Volume: 2 Pages: e370-e370
  • DOI: 10.7717/peerj.370
  • Peer Reviewed / Open Access
• [Journal Article] Lineage-specific expression of Bestrophin-2 and Bestrophin-4 in human intestinal epithelial cells. (2013)
  • Author(s): Ito G, Okamoto R, Nakamura T, Watanabe M et al
  • Journal Title: PloS One. Volume: 8 Issue: 11 Pages: e79693-e79693
  • DOI: 10.1371/journal.pone.0079693
  • Peer Reviewed
• [Journal Article] IL-7 promotes long-term in vitro survival of unique long-lived memory subset generated from mucosal effector memory CD4+ T cells in chronic colitis mice. (2013)
  • Author(s): Takahara M, Nemoto Y, Oshima S, Matsuzawa Y, Kanai T, Okamoto R, Tsuchiya K, Nakamura T, Yamamoto K, Watanabe M.
  • Journal Title: Immunol Lett. Volume: 156 Issue: 1-2 Pages: 82-93
  • DOI: 10.1016/j.imlet.2013.09.001
  • NAID: 120005468269
  • Peer Reviewed
• [Journal Article] Bone marrow-mesenchymal stem cells are a major source of interleukin-7 and sustain colitis by forming the niche for colitogenic CD4+ memory T cells. (2013)
  • Author(s): Nemoto Y, Nakamura T, Watanabe M et al
  • Journal Title: Gut. Volume: 62 Issue: 8 Pages: 1142-1152
  • DOI: 10.1136/gutjnl-2012-302029
  • Peer Reviewed
• [Journal Article] Wnt5a Signaling Mediates Biliary Differentiation of Fetal Hepatic Stem/Progenitor Cells (2013)
  • Author(s): Kakinuma S, Kiyohashi K, Kamiya A, Sakamoto N, Nitta S, Yamanaka H, YoshinoK, Fujiki J, Murakawa M, Kusano-Kitazume A, Shimizu H, Okamoto R, Azuma S, Nakagawa M, Asahina Y, Tanimizu N, Kikuchi A, Nakauchi H, and *WatanabeM
  • Journal Title: Hepatology Volume: (in press) Issue: 6 Pages: 2502-2513
  • DOI: 10.1002/hep.26293
  • Peer Reviewed
• [Journal Article] The acquisition of malignant potential in colon cancer is regulated by the stabilization of Atonal homolog 1 protein. (2013)
  • Author(s): Kano Y
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 432 Issue: 1 Pages: 175-181
  • DOI: 10.1016/j.bbrc.2013.01.034
  • Peer Reviewed / Open Access
• [Journal Article] The development of colitogenic CD4+ T cells is regulated by IL-7 in collaboration with natural killer cell function in a murine model of colitis (2012)
  • Author(s): Yamaji O, Totsuka T
  • Journal Title: J Immunol Volume: 188 Issue: 6 Pages: 2524-2536
  • DOI: 10.4049/jimmunol.1100371
  • Peer Reviewed
• [Journal Article] Endoscopic ultrasound with double-balloon endoscopy for the diagnosis of inverted Meckel'sdiverticulum: a case report (2012)
  • Author(s): Araki A, Tsuchiya K, Oshima S, Okada E,Suzuki S, Akiyama JM, Fujii T, OkamotoR, Watanabe M
  • Journal Title: J Med Case Rep Volume: 6(1) Issue: 1 Pages: 328-328
  • DOI: 10.1186/1752-1947-6-328
  • Peer Reviewed
• [Journal Article] Real-time analysis of P-glycoprotein-mediated drug transport across primary intestinal epithelium three-dimensionally cultured in vitro (2012)
  • Author(s): Mizutani T, Nakamura T, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 419 Issue: 2 Pages: 238-243
  • DOI: 10.1016/j.bbrc.2012.01.155
  • Peer Reviewed
• [Journal Article] Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+stem cell (2012)
  • Author(s): Yui S, Nakamura T, Sato T, Nemoto Y, Mizutani T, Zheng X, Ichinose S, Nagaishi T, Okamoto R, Tsuchiya K, Clevers H, and Watanabe M
  • Journal Title: Nature Medicine Volume: 18 Issue: 4 Pages: 618-623
  • DOI: 10.1038/nm.2695
  • Peer Reviewed
• [Presentation] Establishment of a 3D cell culture-based screening platform to identify natural products that can regulate transepithelial water transport of the human gastrointestinal tract. (2015)
  • Author(s): Satoru Fujii, Ryuichi Okamoto, Toru Nakata, Kohei Suzuki, Fumiaki Ishibashi, Ami Kawamoto,Sayaka Ohashi Segawa, Tomohiro Mizutani, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru Watanabe.
  • Organizer: Pacifichem2015
  • Place of Presentation: ハワイ（米国）
  • Year and Date: 2015-12-17
  • Int'l Joint Research
• [Presentation] TNF-α stabilizes atoh1 protein in colitis-associated colorectal cancer resulting in enhanced malignant potential. (2015)
  • Author(s): Tomoaki Shirasaki, Keita Fukushima, Kiichiro Tsuchiya, Shuuji Hibiya, Ryohei Hayashi, Nobukatsu Horita, Ryuichi Okamoto, Tetsuya Nakamura, Mamoru Watanabe.
  • Organizer: UEGW2015
  • Place of Presentation: バルセロナ（スペイン）
  • Year and Date: 2015-10-28
  • Int'l Joint Research
• [Presentation] Atoh1 protein stabilized by TNF-α acquires enhanced malignant potential in colitis-associated colorectal cancer. (2015)
  • Author(s): Tomoaki Shirasaki, Keita Fukushima, Kiichiro Tsuchiya, Shuuji Hibiya, Ryohei Hayashi, Nobukatsu Horita, Ryuichi Okamoto, Tetsuya Nakamura, Mamoru Watanabe.
  • Organizer: AOCC2015
  • Place of Presentation: 北京（中国）
  • Year and Date: 2015-06-19
  • Int'l Joint Research
• [Presentation] A novel fluorescent labelling system into small intestinal organoid reveals independent long-lived intestinal stem cells in a crypt. (2015)
  • Author(s): Horita N,Tsuchiya K,Hayashi R,Fukushima K,Hibiya S,Fukuda M,Mizutani T,Okamoto R,Nakamura T and Watanabe M.
  • Organizer: AOCC2015
  • Place of Presentation: 北京（中国）
  • Year and Date: 2015-06-19
  • Int'l Joint Research
• [Presentation] Enrichment of Intestinal Stem Cells by the 3D-Culture of IBD Patient Derived Intestinal epithelium. (2015)
  • Author(s): Kohei Suzuki,Satoru Fujii,Toru Nakata,Ami Kawamoto,Fumiaki Ishibashi,Sayaka Ohashi Segawa, Tomohiro Mizutani, Kiichiro Tsuchiya,Kazuo Otsuka,Tetsuya Nakamura,Ryuichi Okamoto,Mamoru Watanabe.
  • Organizer: AOCC2015
  • Place of Presentation: ワシントン（米国）
  • Year and Date: 2015-05-19
  • Int'l Joint Research
• [Presentation] Distinct Role of Notch Ligands, DLL1 and Dll4, in Normal and in Tumor Intestinal Epithelium. (2015)
  • Author(s): Toru Nakata, Hiromichi Shimizu, Kohei Suzuki, Satoru Fujii, Go Ito, Kiichiro Tsuchiya, Tetsuya Nakamura, Ryuichi Okamoto, Katsuto Hozumi, Mamoru Watanabe.
  • Organizer: DDW 2015
  • Place of Presentation: ワシントン（米国）
  • Year and Date: 2015-05-19
  • Int'l Joint Research
• [Presentation] Notch Ligands DLL1 and Dll4 Are Expressed by Distinct Population of Epithelial Cells in the Mice Intestine. (2015)
  • Author(s): Hiromichi Shimizu, Kohei Suzuki, Satoru Fujii, Toru Nakata, Go Ito, Tatsuro Murano, Kiichiro Tsuchiya, Tetsuya Nakamura, Ryuichi Okamoto, Katsuto Hozumi, Mamoru Watanabe.
  • Organizer: DDW 2015
  • Place of Presentation: ワシントン（米国）
  • Year and Date: 2015-05-16
  • Int'l Joint Research
• [Presentation] Notch signaling and TNF-α synergistically up-regulates expression of OLFM4 in human intestinal epithelial cells. (2014)
  • Author(s): Go Ito, Ryuichi Okamoto, Tasturo Murano, Hiromichi Shimizu, Satoru Fujii, Toru Nakata, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru Watanabe
  • Organizer: 第8回Japan-US Collabaration Conference in Gastroenterology
  • Place of Presentation: Intercontinental TOKYO BAY (東京都港区海岸1-16-2)
  • Year and Date: 2014-11-21
• [Presentation] Notch signaling regulates expression of gelsolin superfamily genes, gelsolim and scinderin and promotes re-assembly of action cytoskeleton in human intestinal epithelial cells. (2014)
  • Author(s): Ryuichi Okamoto, Go Ito, Hiromichi Shimizu, Satoru Fujii , Toru Nakata, Kohei Suzuki, Mamoru Watanabe.
  • Organizer: UEGW2014
  • Place of Presentation: Vienna (Austria)
  • Year and Date: 2014-10-22
• [Presentation] Notch Signaling and TNF-alpha synergistically promotes intracellular protein accumulation of olfm4 in the inflamed mucosa of ulcerative colitis. (2014)
  • Author(s): Go Ito, Ryuichi Okamoto, Hiromichi Shimizu, Satoru Fujii, Toru Nakata, Kohei Suzuki, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru Watanabe
  • Organizer: UEGW2014
  • Place of Presentation: Vienna (Austria)
  • Year and Date: 2014-10-21
• [Presentation] Notch signaling and TNF-α synergistically promotes intracellular protein expression of OLFM4 in human intestinal epithelial cells. (2014)
  • Author(s): Go Ito, Ryuichi Okamoto, Hiromichi Shimizu, Satoru Fujii, Toru Nakata, Kohei Suzuki, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru Watanabe.
  • Organizer: The 2nd Annual Meeting of Asian Organization for Crohn's and Colitis
  • Place of Presentation: Seoul (Korea)
  • Year and Date: 2014-06-21
• [Presentation] Distinct Expression Patterns of Notch Ligands, DLL1 and DLL4, in Normal and Inflamed Mice Intestine (2014)
  • Author(s): Hiromichi Shimizu, Ryuichi Okamoto, Go Ito, Satoru Fujii, Toru Nakata, Kohei Suzuki, Tatsuro Murano, Tomohiro Mizutani, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Mamoru Watanabe
  • Organizer: The 2nd Annual Meeting of Asian Organization for Crohn's and Colitis
  • Place of Presentation: Seoul (Korea)
  • Year and Date: 2014-06-21
• [Presentation] 炎症性腸疾患における上皮再生機構の解明と粘膜再生治療への応用 (2013)
  • Author(s): 岡本隆一、村野竜朗、渡辺 守
  • Organizer: 第99回日本消化器病学会総会
  • Place of Presentation: 鹿児島
  • Year and Date: 2013-03-21
• [Presentation] Notch pathway and TNF-a synergistically up-regulates OLFM4 expression in the inflamed mucosa of the human intestine. (2012)
  • Author(s): Okamoto R, Watanabe M et al
  • Organizer: Asian IBD Symposium Seoul 2012
  • Place of Presentation: Seoul
  • Year and Date: 2012-11-03
• [Presentation] Hes1 promotes IL-22-mediated epithelial regeneration through enheancement of STAT3-dependent transcription in human intestinal epithelial cells. (2012)
  • Author(s): Okamoto R, Watanabe M et al
  • Organizer: Asian IBD Symposium Seoul 2012
  • Place of Presentation: Seoul
  • Year and Date: 2012-11-03
• [Presentation] Establishment of novel bio-screening methods using cultured intestinal epithelial cells . (2012)
  • Author(s): Okamoto R
  • Organizer: The 1st International Symposium on Chemical Biology of Natural Products: Target ID and Regulation of Bioactivity
  • Place of Presentation: 京都
  • Year and Date: 2012-10-31
• [Presentation] The acquisition of cancer stemness in colon cancer by the Atoh1 protein stabilization. (2012)
  • Author(s): Kano Y, Okamoto R, Nakamura T, Watanabe M et al
  • Organizer: ISSCR2012
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-14
• [Presentation] Regeneration of damaged colon epithelium by transplanted colon Lgr5+ stem cells maintained and expanded in vitro. (2012)
  • Author(s): Yui S, Nakamura T, Okamoto R, Watanabe M et al
  • Organizer: 第10回 幹細胞シンポジウム
  • Place of Presentation: 淡路島
  • Year and Date: 2012-06-01
• [Presentation] Flagellin response via TLR5 on basolateral membrane of primary intestinal epithelial cells is regulated by Notch signaling. (2012)
  • Author(s): Tsuchiya K, Okamoto R, Nakamura T, Watanabe M et al
  • Organizer: DDW2012
  • Place of Presentation: San Diego
  • Year and Date: 2012-05-22
• [Presentation] Colitogenic effector memory CD4+ T cells develop TH1/TH17 mediated interstitial pneumonia independent to intestinal bacterial antigens. (2012)
  • Author(s): Nemoto Y, Okamoto R, Nakamura T, Watanabe M et al
  • Organizer: DDW2012
  • Place of Presentation: San Diego
  • Year and Date: 2012-05-22
• [Presentation] Notch signaling regulates expression of Gelsolin superfamily genes, Gelsolin and Scinderin, and promotes re-assembly of actin cytoskelton in human intestinal epithelial cells. (2012)
  • Author(s): Okamoto R, Nakamura T, Watanabe M et al
  • Organizer: DDW2012
  • Place of Presentation: San Diego
  • Year and Date: 2012-05-21
• [Presentation] Real-time analysis of p-glycoprotein-mediated drug transport across primary intestinal epitherial cells three-dimensionally cultured in vitro. (2012)
  • Author(s): Mizutani T, Nakamura T, Okamoto R, Watanabe M et al
  • Organizer: DDW2012
  • Place of Presentation: San Diego
  • Year and Date: 2012-05-21
• [Presentation] Hes1 promotes IL-22-Mediated epithelial regeneration through enhancement of STAT3-Dpendent transcription in human intestinal epithelial cells. (2012)
  • Author(s): Murano T, Okamoto R, Nakamura T, Watanabe M et al
  • Organizer: DDW2012
  • Place of Presentation: San Diego
  • Year and Date: 2012-05-19
• [Presentation] The acquisition of cancer stemness in colon cancer by the Atoh1 protein stabilization. (2012)
  • Author(s): Kano Y, Okamoto R, Nakamura T, Watanabe M et al
  • Organizer: DDW2012
  • Place of Presentation: San Diego
  • Year and Date: 2012-05-19
• [Presentation] Regeneration of damaged colonic tissue by transplanted colonic epithelial stem cells maintained and expanded in vitro (2011)
  • Author(s): Yui S., Okamoto R., et al
  • Organizer: GI Research Academy 2011
  • Place of Presentation: 京都
  • Year and Date: 2011-06-17
• [Presentation] Notch-Hes1 pathway and TNF-α synergistically up-regulates OLFM4 expression in the inflamed mucosa of the human intestine (2011)
  • Author(s): Okamoto R., et al
  • Organizer: DDW 2011
  • Place of Presentation: シカゴ
  • Year and Date: 2011-05-07
• [Presentation] Complete conversion of crypt progenitor cells into Atoh1-positive cells by targeted deletion of Dll1 and Dll4 in Lgr5-positive intestinal stem cells
  • Author(s): Hiromichi Shimizu, Ryuichi Okamoto, Satoru Fujii, Go Ito, Tatsuro Murano, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru Watanabe
  • Organizer: DDW 2013
  • Place of Presentation: オーランド、米国
• [Presentation] Notch signaling regulates expression of Bestrophin-4, a novel enterocyte-specific HCO3-/Cl- channel, in human intestinal epithelial cells.
  • Author(s): Go Ito, Ryuichi Okamoto, Satoru Fujii, Hiromichi Shimizu, Tatsuro Murano, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru Watanabe
  • Organizer: DDW 2013
  • Place of Presentation: オーランド、米国
• [Presentation] Hes1 promotes IL-22-mediated antimicrobial response through enhancement of STAT3-dependent transcription in human intestinal epithelial cells
  • Author(s): Ryuichi Okamoto, Tatsuro Murano, Hiromichi Shimizu, Go Ito, Satoru Fujii, Kiichiro Tsuchiya, Tetsuya Nakamura, and Mamoru Watanabe
  • Organizer: The 1st Annual Meeting of Asian Organization for Crohn's and Colitis
  • Place of Presentation: 東京、ラフォーレ東京
• [Presentation] Lineage-specific expression of bestrophin genes in normal and inflamed human intestine: implications for ulcerative colitis.
  • Author(s): Go Ito, Ryuichi Okamoto, Tatsuro Murano, Hiromichi Shimizu, Satoru Fujii, Kiichiro Tsuchiya, Tetsuya Nakamura, and Mamoru Watanabe
  • Organizer: The 1st Annual Meeting of Asian Organization for Crohn's and Colitis
  • Place of Presentation: 東京、ラフォーレ東京
• [Presentation] LINEAGE-SPECIFIC EXPRESSION OF BESTROPHIN-2 AND BESTROPHIN-4 IS REGULATED BY NOTCH SIGNALING IN HUMAN INTESTINAL EPITHELIAL CELLS
  • Author(s): G. Ito, R. Okamoto, T. Murano, H. Shimizu, K. Tsuchiya, T. Nakamura, M. Watanabe
  • Organizer: UEGW 2013
  • Place of Presentation: ベルリン、ドイツ
• [Presentation] COMPLETE CONVERSION OF CRYPT PROGENITOR CELLS INTO ATOH1-POSITIVE CELLS BY TARGETED DELETION OF DLL1 AND DLL4 IN LGR5-POSITIVE INTESTINAL STEM CELLS
  • Author(s): H. Shimizu, R. Okamoto, T. Nakata, S. Fujii, G. Ito, T. Murano, K. Tsuchiya, T. Nakamura, M. Watanabe
  • Organizer: UEGW 2013
  • Place of Presentation: ベルリン、ドイツ
• [Presentation] IL-22-MEDIATED ANTIMICROBIAL RESPONSE IS REGULATED BY HES1 VIA STAT3-DEPENDENT TRANSCRIPTION IN HUMAN INTESTINAL EPITHELIAL CELLS
  • Author(s): R. Okamoto, T. Murano, H. Shimizu, G. Ito, S. Fujii, T. Nakata, K. Tsuchiya, T. Nakamura, M. Watanabe
  • Organizer: UEGW 2013
  • Place of Presentation: ベルリン、ドイツ