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Discovery of natural products that modulate cellular responses

Planned Research

Project AreaChemical Biology using bioactive natural products as specific ligands: identification of molecular targets and regulation of bioactivity
Project/Area Number 23102006
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionKeio University

Principal Investigator

Imoto Masaya  慶應義塾大学, 理工学部, 教授 (60213253)

Co-Investigator(Renkei-kenkyūsha) KAKEYA HIDEAKI  京都大学, 薬学研究科(研究院), 教授 (00270596)
TASHIRO ETSU  慶應義塾大学, 理工学部, 専任講師 (00365446)
Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥65,130,000 (Direct Cost: ¥50,100,000、Indirect Cost: ¥15,030,000)
Fiscal Year 2015: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2014: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2013: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2012: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywordsアンドロゲンアンタゴニスト / オートファジー / βカテニン / アンタルライド / キサントフモール / ノナクチン / 難治疾患 / 天然物リガンド / 作用解析機構 / VCP / がん / パーキンソン病 / 前立腺がん / アポトーシス / EMT / 放線菌培養液 / 神経細胞 / MPP
Outline of Final Research Achievements

In the course of screening for a new AR antagonist, we isolated novel compounds, antarlides A-E, from the Streptomyces sp. BB47. In addition, antarlide B inhibited the transcriptional activity of not only wild type AR but also mutant ARs, which are seen in patients with acquired resistance to clinically used AR antagonists.We next observed that xanthohumol (XN), a prenylated chalcone, modulates autophagy. By using XN-immobilized beads, valosin-containing protein (VCP) was identified as a XN-binding protein. These data indicated that XN inhibited the function of VCP, thereby allowing the impairment of autophagosome maturation. We also screened the compound that induced cell death selectively in tumor cell lines harboring β-catenin mutation from an in-house natural product library, and finally we isolated and found that nonactin. Furthermore, nonactin induced tumor regression only in β-catenin mutated HCT116 xenograft mice.

Report

(6 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (44 results)

All 2016 2015 2014 2013 2012 2011 Other

All Journal Article (19 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 19 results,  Acknowledgement Compliant: 4 results,  Open Access: 5 results) Presentation (21 results) (of which Int'l Joint Research: 1 results,  Invited: 9 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Ulbactins F and G, Polycyclic Thiazoline Derivatives with Tumor Cell Migration Inhibitory Activity from Brevibacillus sp.2016

    • Author(s)
      Igarashi Y, Asano D, Sawamura M, In Y, Ishida T, Imoto M.
    • Journal Title

      Org Lett.

      Volume: 18 Issue: 7 Pages: 1658-1661

    • DOI

      10.1021/acs.orglett.6b00531

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antarlides: A New Type of Androgen Receptor (AR) Antagonist that Overcomes Resistance to AR-Targeted Therapy2016

    • Author(s)
      Saito S, Fujimaki T, Panbangred W, Igarashi Y, Imoto M.
    • Journal Title

      Angew Chem Int Ed Engl.

      Volume: 55 Issue: 8 Pages: 2718-2732

    • DOI

      10.1002/anie.201510079

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Evaluation of drug toxicity profiles based on the phenotypes of ascidian Ciona intestinalis.2015

    • Author(s)
      Mizotani Y, Itoh S, Hotta K, Tashiro E, Oka K, Imoto M.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 463 Issue: 4 Pages: 656-60

    • DOI

      10.1016/j.bbrc.2015.05.119

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] SMK-17, a MEK1/2-specific inhibitor, selectively induces apoptosis in β-catenin-mutated tumors.2015

    • Author(s)
      Kiga M, Nakayama A, Shikata Y, Sasazawa Y, Murakami R, Nakanishi T, Tashiro E, Imoto M.
    • Journal Title

      Scientific Report

      Volume: 5 Issue: 1 Pages: 8155-8155

    • DOI

      10.1038/srep08155

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 5-Lipoxygenase and CysLT1 regulate EGF‐induced cell migration through Tiam1 upregulation and Rac1 activation.2014

    • Author(s)
      Magi S, Takemoto Y, Kobayashi H, Kasamatsu M, Akita T, Tanaka A, Takano K, Tashiro E, Igarashi Y, Imoto M.
    • Journal Title

      Cancer Science

      Volume: 105 Issue: 3 Pages: 290-296

    • DOI

      10.1111/cas.12340

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chemical genomic-based pathway analyses for epidermal growth factor-mediated signaling in migrating cancer cells.2014

    • Author(s)
      Magi S, Saeki Y, Kasamatsu M, Tashiro E, Imoto M.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 5 Pages: e96776-e96776

    • DOI

      10.1371/journal.pone.0096776

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of licopyranocoumarin and glycyrurol from herbal medicines as neuroprotective compounds for Parkinson’s disease.2014

    • Author(s)
      Fujimaki T, Saiki S, Tashiro E, Yamada D, Kitagawa M, Hattori N and Imoto M.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 6 Pages: e100395-e100395

    • DOI

      10.1371/journal.pone.0100395

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Xanthohumol suppresses oestrogen-signalling in breast cancer through the specific inhibition of BIG3-PHB2 interaction.2014

    • Author(s)
      Yoshimaru T, Komatsu M, Tashiro E, Imoto M, Osada H, Miyoshi M, Honda J, Sasa M, and Katagiri T
    • Journal Title

      Scientific Report

      Volume: 4 Issue: 1 Pages: 7335-7335

    • DOI

      10.1038/srep07355

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] HCV NS3 protease enhancesliver fibrosis via binding to and activating TGF-β type I receptor2013

    • Author(s)
      Sakata K, Eda S, Lee E, Hara M, Imoto M, Kojima S.
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 3243-3243

    • DOI

      10.1038/srep03243

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of a new detection system for the dimerization of IRE1α with BiFC method.2013

    • Author(s)
      Shinjo S, Tashiro E, Imoto M.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 77 Pages: 1333-1336

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Comparative Analysis of the Expression Patterns of UPR-Target Genes caused by UPR-inducing Compounds.2013

    • Author(s)
      Shinjo S, Mizotani Y, Tashiro E, Imoto M.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 77 Pages: 729-735

    • NAID

      10031170710

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel derivatives of aclacinomycin A block cancer cell migration through inhibition of farnesyl transferase2013

    • Author(s)
      Shigeyuki Magi, Tetsuo Shitara, Yasushi Takemoto, Masato Sawada, Mitsuhiro Kitagawa, Etsu Tashiro, Yoshikazu Takahashi, Masaya Imoto
    • Journal Title

      The Journal of Antibiotics

      Volume: (掲載決定) Issue: 3 Pages: 165-170

    • DOI

      10.1038/ja.2012.108

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A chemical genomic study identifying diversity in cell migration signaling in cancer cells.2012

    • Author(s)
      Magi S, Tashiro E and Imoto M.
    • Journal Title

      Scientific Reports

      Volume: 2 Issue: 1 Pages: 823-823

    • DOI

      10.1038/srep00823

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inostamycin enhanced TRAIL-induced apoptosis through DR5 up-regulation on the cell surface2012

    • Author(s)
      K. Yamamoto, M. Makino, R. Watanapokasin, E. Tashiro, and M. Imoto
    • Journal Title

      The Journal of Antibiotics

      Volume: 65 Issue: 6 Pages: 295-300

    • DOI

      10.1038/ja.2012.21

    • NAID

      10030797925

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Xanthohumol impairs autophagosome maturation through direct inhibition of valosin-containing protein2012

    • Author(s)
      Sasazawa, Y., Kanagaki, S., Tashiro, E., Nogawa, T., Muroi, M., Kondoh, Y., Osada, H., Imoto, M
    • Journal Title

      ACS Chemical Biology

      Volume: (in press) Issue: 5 Pages: 892-900

    • DOI

      10.1021/cb200492h

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Xanthohumol Impairs Autophagosome Maturation through Direct Inhibition of Valosin-Containing Protein2012

    • Author(s)
      Y.Sasazawa, S.Kanagaki, E.Tashiro, T.Nogawa, M.Muroi, Y.Kondoh, H.Osada, M.Imoto
    • Journal Title

      ACS Chemical Biology

      Volume: (in press)(掲載決定)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of 14-3-3 proteins in the second EGF-induced wave of Rac1 activation in the process of cell migration2011

    • Author(s)
      H.Kobayashi, Y.Ogura, M.Sawada, R.Nakayama, K.Takano, Y.Minato, Y.Takemoto, E.Tashiro, H.Watanabe, M.Imoto
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Pages: 39259-39268

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of "Unnatural Natural Product" library and identification of a small molecule inhibitor of XIAP2011

    • Author(s)
      T.Kawamura, K.Matsubara, H.Otaka, E.Tashiro, K.Shindo, R.C.Yanagita, K.Irie, M.Imoto
    • Journal Title

      Bioorg.& Med.Chem.

      Volume: 19 Pages: 4377-4386

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and anti-migrative evaluation of moverastin derivatives2011

    • Author(s)
      M.Sawada, S.Kubo, K.Matsumura, Y.Takemoto, H.Kobayashi, E.Tashiro, T.Kitahara, H.Watanabe, M.Imoto
    • Journal Title

      Bioorg.c & Med.l Chem.Lett.

      Volume: 21 Pages: 1385-1389

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] ホルモン療法抵抗性前立腺がんに対する天然物創薬研究2016

    • Author(s)
      齋藤駿、藤巻貴宏、田代悦、五十嵐康弘、井本正哉
    • Organizer
      日本薬学会第136回年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Isolation and biological activities of novel androgen receptor antagonist produced by Streptomyces2015

    • Author(s)
      S.Saito, T.Fujimaki, E.Tashiro, Y.Igarashi, M.Imoto
    • Organizer
      PACIFICHEM 2015
    • Place of Presentation
      ホノルル(米国)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 放線菌由来新規アンドロゲン受容体アンタゴニスト Antarlide 類の単離・構造決定及び生物活性2015

    • Author(s)
      齋藤駿、藤巻貴宏、田代悦、五十嵐康弘、井本正哉
    • Organizer
      第57回天然有機化合物討論会
    • Place of Presentation
      神奈川県民ホール(神奈川県・横浜市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 微生物由来天然化合物の治療薬シードへの展開2015

    • Author(s)
      井本正哉、田代悦
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学 津島キャンパス(岡山県岡山市)
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Chemistry and biology of natural products that modulate cellular responses2015

    • Author(s)
      Masaya Imoto
    • Organizer
      SIBM Natural Products 2015
    • Place of Presentation
      San Diego, CA USA
    • Year and Date
      2015-01-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 5-リポキシゲナーゼ/CysLT1シグナルによるがん細胞遊走促進機構解析2014

    • Author(s)
      井本正哉
    • Organizer
      第23回日本がん転移学会学術集会・総会
    • Place of Presentation
      金沢市文化ホール(石川県金沢市)
    • Year and Date
      2014-07-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新規アンドロゲン受容体アンタゴニストの探索と薬理活性評価2014

    • Author(s)
      齋藤駿、藤巻貴宏、榊原康文、田代悦、井本正哉
    • Organizer
      日本ケミカルバイオロジー学会第9回年会
    • Place of Presentation
      大阪大学 大阪大学会館(大阪府豊中市)
    • Year and Date
      2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Xanthohumol Impairs Autophagosome Maturation through Direct Inhibition of Valosin-Containing Protein2014

    • Author(s)
      Masaya Imoto
    • Organizer
      Riken-MaxPlanck 3rd Annual Symposium
    • Place of Presentation
      Kreuth/German
    • Year and Date
      2014-05-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ヒト大腸がん細胞LoVoにおける、EGF誘導体E-cad-herin発現抑制機構の解析2013

    • Author(s)
      大嶽弘之、邊見静香、久田明里、小林大貴、田代悦、井本正哉
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] 癌の代謝解析による薬剤標的探索2013

    • Author(s)
      井本正哉
    • Organizer
      がんと代謝シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2013-01-08
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Studies on the Signaling Pathway for Cancer Cell Migration by the Combination of Chemical Biology and Systems Biology2012

    • Author(s)
      Shigeyuki Magi, Etsu Tashiro, and Masaya Imoto
    • Organizer
      FOSBE 2012
    • Place of Presentation
      鶴岡
    • Year and Date
      2012-10-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] Drug Seed Generation from Natural Products2012

    • Author(s)
      Masaya Imoto
    • Organizer
      3rd MEXT-ARDA PROJECT Concluding Joint Seminar and JSPS-NRCT ASIAN CORE PROGRAM Joint Seminar
    • Place of Presentation
      Bangkok(Thailand)
    • Year and Date
      2012-08-26
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 新たなアンドロゲンアンタゴニストの検索のためのアプローチ2012

    • Author(s)
      藤巻貴宏、田代悦、井本正哉
    • Organizer
      第16回日本がん分子標的治療学会
    • Place of Presentation
      小倉
    • Year and Date
      2012-06-29
    • Related Report
      2012 Annual Research Report
  • [Presentation] 新規アンドロゲンアンタゴニストの探索と薬理活性評価2011

    • Author(s)
      藤巻貴宏、鳥居健太郎, 他、井本正哉
    • Organizer
      日本ケミカルバイオロジー学会第6回年会
    • Place of Presentation
      東京工業大学(東京)
    • Year and Date
      2011-05-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新規MEK1/2阻害剤、SMK-17の抗腫瘍活性2011

    • Author(s)
      木我真基、中山綾子, 他、井本正哉
    • Organizer
      日本ケミカルバイオロジー学会第6回年会
    • Place of Presentation
      東京工業大学(東京)
    • Year and Date
      2011-05-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] イノスタマイシンによるFGF受容体-1ゴルジ体蓄積機構の解析2011

    • Author(s)
      田代悦、張嘉峯、井本正哉
    • Organizer
      日本ケミカルバイオロジー学会第6回年会
    • Place of Presentation
      東京工業大学(東京)
    • Year and Date
      2011-05-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Chemistry and biology of Xanthohumol, an inhibitor of Valosin-Containing Protein.

    • Author(s)
      井本正哉
    • Organizer
      EMBO Workshop【AAA+ proteins: from mechanism and disease to targets】
    • Place of Presentation
      Neuss, Germany
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] オートファジーに挑むケミカルバイオロジー

    • Author(s)
      井本正哉
    • Organizer
      新規素材探索研究会 第12回セミナー
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ホップ成分キサントフモールのオートファジー制御機構と抗がん活性評価

    • Author(s)
      井本正哉
    • Organizer
      第5回食品薬学シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 天然化合物を用いたケミカルバイオロジー研究

    • Author(s)
      井本正哉
    • Organizer
      日本薬学会第134年会シンポジウム
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 日本における天然物創薬の現状

    • Author(s)
      井本正哉
    • Organizer
      日本農芸化学会2014年度大会シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] カレントレビュー192015

    • Author(s)
      井本正哉
    • Total Pages
      189
    • Publisher
      化学同人
    • Related Report
      2014 Annual Research Report
  • [Book] 最新がん薬物療法学2015

    • Author(s)
      田代悦,井本正哉
    • Total Pages
      704
    • Publisher
      (株)日本臨床社
    • Related Report
      2014 Annual Research Report
  • [Book] オートファジーに挑むケミカルバイオロジー 「天然物化学とケミカルバイオロジーの挑戦」2014

    • Author(s)
      北川光洋,田代悦,井本正哉
    • Total Pages
      79
    • Publisher
      (株)化学工業社
    • Related Report
      2014 Annual Research Report
  • [Remarks] 慶應義塾大学理工学部生命情報学科ケミカルバイオロジー研究室

    • URL

      http://www.bio.keio.ac.jp/labs/imoto/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-08-01   Modified: 2019-07-29  

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