| Budget Amount *help |
¥43,290,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥9,990,000)
Fiscal Year 2015: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Outline of Final Research Achievements |
The third-generation small-molecule affinity matrix has been developed to facilitate target protein identification of bioactive small molecules. This newly developed photoactivatable and photocleavable affinity matrix enabled quantification of the amount and distribution of immobilized small molecules prior to the pull-down experiments to identify target protein for the immobilized small molecules. We also performed structure-activity relationship studies and target identification studies of bioactive natural products such as cytotoxic macrolactam natural products heronamides and vicenistatin, cytotoxic macrolide FD-891, osteoclastogenesis inhibitor methyl gerfelin, and anticancer polyketides irciniastatins.
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