| Budget Amount *help |
¥120,640,000 (Direct Cost: ¥92,800,000、Indirect Cost: ¥27,840,000)
Fiscal Year 2015: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2014: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2013: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2012: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥50,700,000 (Direct Cost: ¥39,000,000、Indirect Cost: ¥11,700,000)
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| Outline of Final Research Achievements |
In order to extract the pure deformability characteristics of cells, it is necessary to improve the resolution of cell actuation in the sensing system. This work discussed how to achieve a high speed cell manipulation with high resolution under a micro fluidic chip. A novel concept in this work is the flow reduction mechanism, where a flow is generated by a macro actuator placed outside of a micro fluidic chip. The flow can be drastically reduced at the cell manipulation point in a micro channel due to the elasticity embedded into the micro fluidic chip. By using this advantage, we could construct a high resolution cell manipulation system and successfully achieved the cell positioning based on a visual feedback control with a resolution of 240nm, together with the maximum frequency of 130Hz.
As an application example, we showed a new concept of cell fatigue evaluation where we continuously impart mechanical stress to the cell until it eventually get a damage.
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