| Budget Amount *help |
¥112,840,000 (Direct Cost: ¥86,800,000、Indirect Cost: ¥26,040,000)
Fiscal Year 2015: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2014: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Fiscal Year 2013: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
Fiscal Year 2012: ¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2011: ¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
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| Outline of Final Research Achievements |
We have developed cell-cultured microfabricated microplates for manipulating adherent cells. We succeeded in three-dimensional manipulation of the adherent cells by handling the microplates with micro-tweezers, fluid flows, magnetic fields and cell traction force. In addition, we have also developed cell-cultured microplates connected with hinges, and constructed three-dimensional cellular structures by folding the microplates by the cell traction force. By embedding permalloy pieces in the cell-cultured microplates, we achieved magnetic handling of the cells on the microplates without physical contacts to both cells and microplates. Using the magnetized microplates, we developed a vertical confocal observation system. This system enables us to observe the boundary of cell membrane at a high-resolution. These progresses will contribute to construction of three-dimensional cellular structures, analysis of cellular interaction, and high-resolution observation of cells.
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