|Budget Amount *help
¥99,190,000 (Direct Cost: ¥76,300,000、Indirect Cost: ¥22,890,000)
Fiscal Year 2015: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2013: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2012: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2011: ¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
|Outline of Final Research Achievements
In this study, we showed that the cationic copolymer also enhances activity of an MNAzyme-type DNA sensor derived from the 10-23 DNAzyme and increased MNAzyme sensitivity. Furthermore, the copolymer enabled us to shorten the substrate-binding arms of the MNAzyme, decreasing the optimum temperature of the MNA assay from 50 °C to physiological temperature.
It was also demonstrated that the copolymer enhanced function of membrane disrupting peptides by facilitating their folding. Effective cytosolic delivery of macromolecular substances would be possible by using peptide/copolymer complex.