|Budget Amount *help
¥97,370,000 (Direct Cost: ¥74,900,000、Indirect Cost: ¥22,470,000)
Fiscal Year 2015: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2014: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2013: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2012: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
Fiscal Year 2011: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
|Outline of Final Research Achievements
The aim of this study is manipulation of cellular function through the modification of cell surface. We used single-stranded DNA, which was conjugated both to poly(ethylene glycol) and to a terminal phospholipid (ssDNA-PEG-lipid) for cell surface modification. The cell surface of cells can be further functionalized through DNA hybridization. We examined control of 1) biological responses and 2) cell-substrate and cell-cell attachment.
1) The modification of cell surface of endothelial cells with antioxidant-loaded liposomes reduced oxidative stress during ischemia reperfusion. The modification of cell aggregates with superparamagnetic iron oxide nanoparticles allowed for MRI monitoring post-transplantation.
2) We realized cell attachment to poly(lactic acid) scaffold in a spatially controlled manner by taking advantage of variety of DNA sequence. In addition, both cell attachment and detachment can be programmed using DNA containing the cleavage site for restriction enzyme.