Studies on biosynthetic machineries for cyclic terpenoids and nucleoside antibiotics
Project Area | Biosynthetic machinery: Deciphering and regulating the system for bioactive metabolite diversification |
Project/Area Number |
23108101
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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Allocation Type | Single-year Grants |
Review Section |
Science and Engineering
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Research Institution | Hokkaido University |
Principal Investigator |
DAIRI Tohru 北海道大学, 工学(系)研究科(研究院), 教授 (70264679)
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Co-Investigator(Renkei-kenkyūsha) |
KATO Nobuo 大阪大学, 産業科学研究所, 教授 (50150537)
SATOH Yasuharu 北海道大学, 大学院工学研究院, 助教 (30360928)
ARAI Ryouichi 信州大学, ファイバーナノテク国際若手研, 助教 (50344023)
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Project Period (FY) |
2011-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥33,540,000 (Direct Cost: ¥25,800,000、Indirect Cost: ¥7,740,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2012: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
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Keywords | 生合成 / インドールジテルペン / 糸状菌 / プレニルトランスフェラーゼ / フタロシン / ペプチドリガーゼ / パキシリン / ポリオキシン / ジテルペン / フシコクシン |
Outline of Final Research Achievements |
1. Fusicoccin A is a diterpene glucoside produced by the fungus Phomopsis amygdali and its derivative lacking the OH group at the 12-position is revealed to be a potential anti-cancer drug. We therefore constructed a mutant producing the desirable intermediate by biosynthetic engineering. 2. Three fungal prenyltransferases, PaxC, PaxD, and AtmD, all of which are responsible for biosynthesis of indole diterpene compounds, were characterized. 3. Aminodeoxyfutalosine is the first intermediate in the new menaquinone biosynthetic pathway. We demonstrated that a radical S-adenosyl methionine enzyme (MqnE) catalyzed the addition of the adenosyl radical to the double bond of 3-[(1-carboxyvinyl)oxy]benzoic acid derived from chorismate by MqnA. 4. During the biosynthetic studies of pheganomycin, we identified a new ATP-grasp enzyme, which phosphorylated the non-proteogenic amino acids with ATP and the successive nucleophilic attack of the peptides.
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Report
(5 results)
Research Products
(57 results)
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[Journal Article] A peptide ligase and the ribosome cooperate to synthesize the peptide pheganomycin.2015
Author(s)
Noike, M., Matsui, T., Ooya, K., Sasaki, I., Ohtaki, S., Hamano, Y., Maruyama, C., Ishikawa, J., Satoh, Y., Ito, H., Morita, H. and Dairi T.
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Journal Title
Nature Chemical Biology
Volume: 11
Issue: 1
Pages: 71-76
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Reconstitution of biosynthetic machinery for indole-diterpene paxilline in Aspergillus oryzae.2013
Author(s)
K. Tagami, C. Liu, A. Minami, M. Noike, T. Isaka, S. Fueki, Y. Shichijo, H. Toshima, K, T. Dairi, and H. Oikawa.
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Journal Title
J. Am. Chem. Soc.
Volume: 135
Issue: 4
Pages: 1260-1263
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular breeding of a fungus producing a precursor diterpene suitable for semi-synthesis by dissection of the biosynthetic machinery2012
Author(s)
M. Noike, Y. Ono, Y. Araki, R. Tanio, Y. Higuchi, H. Nitta, Y. Hamano, T. Toyomasu, T. Sassa, N. Kato, and T. Dairi
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Journal Title
PLoS ONE
Volume: -
Issue: 8
Pages: e42090-e42090
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] リボソームと新規ペプチドライゲースによる協同的ペプチド生成機構2014
Author(s)
野池基義, 松井 崇, 雄谷洸一, 佐々木郁雄, 丸山千登勢, 濱野吉十, 石川 淳, 佐藤康治, 伊藤 肇, 森田洋行, 大利 徹
Organizer
第56回天然有機化合物討論会
Place of Presentation
高知県立県民文化ホール(高知県高知市)
Year and Date
2014-10-15 – 2014-10-17
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