| Budget Amount *help |
¥127,010,000 (Direct Cost: ¥97,700,000、Indirect Cost: ¥29,310,000)
Fiscal Year 2015: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2014: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2013: ¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
Fiscal Year 2012: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2011: ¥35,100,000 (Direct Cost: ¥27,000,000、Indirect Cost: ¥8,100,000)
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| Outline of Final Research Achievements |
Glycosylation is one of the major post-translational protein modifications. Recently, it has become increasing clear that glycans are involved in higher brain function including neural plasticity. In this study, we mainly focused on elucidating the functional roles of N-glycans expressed on AMPA receptors (AMPAR) and HNK-1 glyco-epitope expressed in perineuronal nets (PNNs). AMPAR and PNNs are known to be key players in the regulation of neural plasticity. Using a series of mutants lacking potential N-glycosylation sites on AMPA-type glutamate receptors, we demonstrated that site-specific N-glycans have the potential to regulate AMPA receptor functions including the cell surface expression, channel property, and lateral diffusion. We also identified a unique HNK-1 glyco-epitope on aggrecan in PNNs. The HNK-1 epitope had an ability to suppress the chondroitin sulfate chains, which play an important role in neural plasticity.
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