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Functional consequences of glia-neuron interactions in degenerating and regenerating neurons

Planned Research

Project AreaBrain Environment
Project/Area Number 23111007
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagoya University

Principal Investigator

Kiyama HIroshi  名古屋大学, 医学(系)研究科(研究院), 教授 (00192021)

Co-Investigator(Kenkyū-buntansha) KIRYU Sumiko  名古屋大学, 大学院医学系研究科, 准教授 (70311529)
KONISHI Hiroyuki  名古屋大学, 大学院医学系研究科, 助教 (90448746)
Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥127,400,000 (Direct Cost: ¥98,000,000、Indirect Cost: ¥29,400,000)
Fiscal Year 2015: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2014: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2013: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
Fiscal Year 2012: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2011: ¥35,230,000 (Direct Cost: ¥27,100,000、Indirect Cost: ¥8,130,000)
Keywords神経損傷 / 運動ニューロン / グリア / ATF3 / DINE / 軸索再生 / 先天性間接拘縮症 / DA5D / ECEL1 / 国際研究者交流 / 国際情報交換(イギリス) / プロテーオミクス / トランスジェニック
Outline of Final Research Achievements

A disruption of intercellular communications among glial cells and neuron after nerve injury leads to neuronal death. We have examined molecules, which mediate interactions between glial cells and injured neurons. We have identified that DINE (damage induced neuronal endopeptidase) and PAP-III/RegIII-g (an inflammatory associated lectin-like protein) are implicated in neuron-Schwann cell interactions during nerve regeneration and development. In addition we have proved that DAP12, which is expressed by microglia, prolongs inflammatory response and is a exacerbating signal for injured motor neurons. We also succeeded in making BAC transgenic mouse Tg(ATF3-mtGPF/Cre), which expresses Cre and mitochondria localizing GFP under ATF3 promoter. This could be a useful tool for future analysis in our project as well as collaborations with other members in A01, A02 and A03.

Report

(6 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (35 results)

All 2016 2015 2014 2013 2012 2011 Other

All Int'l Joint Research (2 results) Journal Article (16 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 13 results,  Acknowledgement Compliant: 5 results,  Open Access: 5 results) Presentation (17 results) (of which Int'l Joint Research: 1 results,  Invited: 9 results)

  • [Int'l Joint Research] Kyung Hee University/Dong-A University(韓国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Damanhour University(エジプト)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] ECEL1 mutation implicates impaired axonal arborization of motor nerves in the pathogenesis of distal arthrogryposis2016

    • Author(s)
      Kenichi Nagata, Sumiko Kiryu-Seo, Hiromi Tamada, Fumi Okuyama-Uchimura, Hiroshi Kiyama, Takaomi C. Saido
    • Journal Title

      Acta Neuropathologica

      Volume: 印刷中 Issue: 1 Pages: 1-16

    • DOI

      10.1007/s00401-016-1554-0

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Ontogeny and innervation of taste buds in mouse palatal gustatory epithelium2016

    • Author(s)
      Rashwan A, Konishi H, El-Sharaby A, Kiyama H
    • Journal Title

      J Chemical Neuroanatomy

      Volume: 71 Pages: 26-40

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Increase of transcription factor EB (TFEB) and lysosomes in rat DRG neurons and their transportation to the central nerve terminal in dorsal horn after nerve injury2016

    • Author(s)
      Jung J, Uesugi N, Jeong NY, Park BS, Konishi H, Kiyama H
    • Journal Title

      Neuroscience

      Volume: 313 Pages: 10-22

    • DOI

      10.1016/j.neuroscience.2015.11.028

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Existence of c-Kit negative cells with ultrastructural features of interstitial cells of Cajal in the subserosal layer of the <i>W/W<sup>v</sup></i> mutant mouse colon2015

    • Author(s)
      Tamada H, Kiyama H
    • Journal Title

      Journal of Smooth Muscle Research

      Volume: 51 Issue: 0 Pages: 1-9

    • DOI

      10.1540/jsmr.51.1

    • NAID

      130005071306

    • ISSN
      0916-8737, 1884-8796
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A DAP12-Dependent Signal Promotes Pro-Inflammatory Polarization in Microglia Following Nerve Injury and Exacerbates Degeneration of Injured Neurons.2015

    • Author(s)
      Kobayashi M, Konishi H, Takai T, Kiyama H.
    • Journal Title

      Glia

      Volume: 63 Issue: 6 Pages: 1073-1082

    • DOI

      10.1002/glia.22802

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Peripheral and spinal mechanisms of nociception in a rat reserpine-induced pain model.2015

    • Author(s)
      Taguchi T, Katanosaka K, Yasui M, Hayashi K, Yamashita M, Wakatsuki K, Kiyama H, Yamanaka A, Mizumura K.
    • Journal Title

      Pain

      Volume: 156 Issue: 3 Pages: 415-427

    • DOI

      10.1097/01.j.pain.0000460334.49525.5e

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A chronic fatigue syndrome model demonstrates mechanical allodynia and muscular hyperalgesia via spinal microglial activation2014

    • Author(s)
      Yasui M, Yoshimura T, Takeuchi S, Tokizane K, Tsuda M, Inoue K, Kiyama H
    • Journal Title

      Glia

      Volume: 62 Issue: 9 Pages: 1407-1417

    • DOI

      10.1002/glia.22687

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Down-regulation of KCC2 expression and phosphorylation in motoneurons, and increases the number of in primary afferent projections to motoneurons in mice with post-stroke spasticity.2014

    • Author(s)
      Toda T, Ishida K, Kiyama H, Yamashita T, Lee S
    • Journal Title

      PLos One

      Volume: 9 Issue: 12 Pages: 1-18

    • DOI

      10.1371/journal.pone.0114328

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] microRNA-124 is down regulated in nerve injured motor neurons and it potentially targets mRNAs for KLF6 and STAT32014

    • Author(s)
      Nagata K, Hama I, Kiryu-Seo S and Kiyama H
    • Journal Title

      Neuroscience

      Volume: 256 Pages: 426-432

    • DOI

      10.1016/j.neuroscience.2013.10.055

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Weakened rate-dependent depression of Hoffmann’s reflex and increased motoneuron hyperactivity after motor cortical infarction in mice2014

    • Author(s)
      Lee S, TodaT, Kiyama H, and Yamashita T
    • Journal Title

      Cell Death & Disease

      Volume: 5 Issue: 1 Pages: e1007-e1007

    • DOI

      10.1038/cddis.2013.544

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nociception originating from the crural fascia in rats2013

    • Author(s)
      Taguchi T, Yasui M, Kubo A, Abe M, Kiyama H, Yamanaka A, Mizumura K
    • Journal Title

      Pain

      Volume: 154 Issue: 7 Pages: 1103-1114

    • DOI

      10.1016/j.pain.2013.03.017

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 脳内環境を制御するミクログリア2013

    • Author(s)
      木山博資
    • Journal Title

      Medical Science Digest (MSD)

      Volume: 39 Pages: 207-210

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
  • [Journal Article] N-terminal Cleaved Pancreatitis-associated Protein-III (PAP-III) Serves as a Scaffold for Neurites and Promotes Neurite Outgrowth.2013

    • Author(s)
      H. Konishi, S. Mateumoto, K. Namikawa, H. Kiyama
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 288(15) Issue: 15 Pages: 10205-10213

    • DOI

      10.1074/jbc.m112.395301

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 損傷神経の生存と軸索再生の分子基盤2013

    • Author(s)
      木山博資
    • Journal Title

      日本精神神経薬理学会

      Volume: 33 Pages: 11-16

    • NAID

      10031169387

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 損傷運動ニューロンの再生・変性とグリア・ニューロン連関2012

    • Author(s)
      木山博資
    • Journal Title

      臨床神経

      Volume: 52 Pages: 934-936

    • NAID

      130004505216

    • Related Report
      2012 Annual Research Report
  • [Journal Article] The nuclear events guiding successful nerve regeneration2011

    • Author(s)
      Kiryu-Seo S, Kiyama H
    • Journal Title

      Front Mol Neurosci

      Volume: 520 Pages: 1-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] The mitochondrial dynamics after nerve injury2015

    • Author(s)
      Kiryu-Seo S and Kiyama H
    • Organizer
      25th International Society for Neurochemistry (ISN)
    • Place of Presentation
      Cairns Australia
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 損傷神経再生の最先端 -自律性と非自律性-2015

    • Author(s)
      木山博資
    • Organizer
      老化促進モデルマウス(SAM)研究協議会
    • Place of Presentation
      岐阜大学サテライトキャンパス、岐阜県、岐阜市
    • Year and Date
      2015-07-04
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Chronic stress and Pain2015

    • Author(s)
      木山博資
    • Organizer
      第120回日本解剖学会第92回日本生理学会合同大会
    • Place of Presentation
      神戸国際会議場 (兵庫県、神戸市)
    • Year and Date
      2015-02-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 神経損傷に応答するミトコンドリア2014

    • Author(s)
      桐生寿美子、木山博資
    • Organizer
      第58回日本神経化学会大会
    • Place of Presentation
      奈良文化会館 (奈良県、奈良市)
    • Year and Date
      2014-09-29 – 2014-10-01
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The protease activity of DINE/ECEL1 is required for the motor nerve terminal arborization and the neuromuscular junction formation2014

    • Author(s)
      Matsumoto S, Kiryu-Seo S, Kiyama H.
    • Organizer
      6th special conference of the international society for neurochemistry
    • Place of Presentation
      東大弥生会館 (東京都文京区)
    • Year and Date
      2014-09-19 – 2014-09-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Aberrant axonal arborization of motor nerves in Damage-Induced Neuronal Endopeptidase deficient limb2014

    • Author(s)
      Nagata K, Kiryu-Seo S, Kiyama H, Saido TC.
    • Organizer
      Cold spring harbor meeting
    • Place of Presentation
      New York, USA
    • Year and Date
      2014-09-16 – 2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 神経損傷後のミクログリア活性化制御因子2014

    • Author(s)
      小西博之,小林正明,木山博資
    • Organizer
      第37回日本神経科学学会
    • Place of Presentation
      パシフィコ横浜 (神奈川県、横浜市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 損傷神経細胞の再生能力に関わる分子メカニズム2013

    • Author(s)
      桐生寿美子、木山博資
    • Organizer
      第118回 日本解剖学会総会・全国学術集会
    • Place of Presentation
      かがわ国際会議場、高松
    • Year and Date
      2013-03-28
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 慢性ストレスによる中枢神経を起点とした恒常性維持の破綻2013

    • Author(s)
      木山博資
    • Organizer
      第86回日本薬理学会
    • Place of Presentation
      福岡国際会議場、福岡
    • Year and Date
      2013-03-21
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 教育口演:グリアの機能形態学2013

    • Author(s)
      木山博資
    • Organizer
      Neuro2013 (第56回日本神経化学会総会)
    • Place of Presentation
      京都国際会館、京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Axonal DINE affects the axon-Schwann cell interactions to form appropriate nerve terminal arborization and neuromuscular junction.2013

    • Author(s)
      Kiryu-Seo S, Matsumoto S and Kiyama H
    • Organizer
      Neuro2013 (第56回日本神経化学会総会)
    • Place of Presentation
      京都国際会館、京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Siglec-H is an anti-inflammatory molecule expressed in microglia in response to nerve injury2013

    • Author(s)
      Kobayashi M, Konishi H, Kiyama H
    • Organizer
      Neuro2013 (第56回日本神経化学会総会)
    • Place of Presentation
      京都国際会館、京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] Aberrant muscle innervation of motoneurons in Damage Induced Neuronal Endopeptidase (DINE) deficient mice2013

    • Author(s)
      Nagata K, Kiryu-Seo S, Saito T, Kiyama H, and Saido TC
    • Organizer
      Neuro2013 (第56回日本神経化学会総会)
    • Place of Presentation
      京都国際会館、京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] 損傷運動ニューロンの再生と変性の転帰に関与するグリア・ニューロン連関2012

    • Author(s)
      木山博資
    • Organizer
      第53回日本神経学会学術大会
    • Place of Presentation
      東京国際フォーラム、東京
    • Year and Date
      2012-05-22
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Nerve injury induced motor neuron death with reference to neuron-glia interaction2012

    • Author(s)
      木山博資
    • Organizer
      第117回日本解剖学会
    • Place of Presentation
      山梨大学、甲府市(シンポジウム)(招待講演)
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Consequences of glia-neuron interaction impairments in nerve2011

    • Author(s)
      木山博資
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜パシフィコ、横浜(シンポジウム)(招待講演)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 脳内環境の破綻を制御する新たなグリア・神経間応答機構の探索とその機能解析2011

    • Author(s)
      木山博資
    • Organizer
      脳内環境総括班会議
    • Place of Presentation
      KKR熱海、熱海市
    • Year and Date
      2011-01-29
    • Related Report
      2011 Annual Research Report

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Published: 2011-08-01   Modified: 2019-07-29  

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