| Budget Amount *help |
¥95,030,000 (Direct Cost: ¥73,100,000、Indirect Cost: ¥21,930,000)
Fiscal Year 2015: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2014: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2013: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2012: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2011: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
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| Outline of Final Research Achievements |
In this study, we investigated the roles of Rho-GEFs, Farp1 and Solo, which are involved in mechanoresponses, in the ordering and the tubule formation of epithelial cells. We found that Farp1 facilitates the adhesion-dependent growth of mammary epithelial cell through integrins. We found that Solo binds to a simple epithelium-specific intermediate filament, keratin8 and keratin18, and Solo is required to organize and maintain of the keratin8/18 network in epithelial cells. We also showed that Solo is required to activate RhoA and to generate the resistance force in response to tensile-force application. Furthermore, we showed that knockdown of Solo accelerates the velocity of collective cell migration of MDCK cells, and enlarges the lumen area of HGF-induced tubule of 3D-cultured MDCK cells.
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