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Modulation of chromatin structure on the non-coding DNA through retrotransposons

Planned Research

Project AreaFunctions of non-coding DNA region for genome integrity
Project/Area Number 23114006
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionTokyo Institute of Technology

Principal Investigator

Kajikawa Masaki  東京工業大学, 生命理工学研究科, 講師 (90361766)

Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥73,450,000 (Direct Cost: ¥56,500,000、Indirect Cost: ¥16,950,000)
Fiscal Year 2015: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2014: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2013: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2012: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2011: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Keywords転移因子 / レトロトランスポゾン / DNAメチル化 / DNAメチル化 / クロマチン / サイレンシング
Outline of Final Research Achievements

We developed a novel system inducing a new insertion of retrotransposons in zebrafish in vivo. Using this system, we revealed that the transcription of the newly inserted retrotransposons has not been silenced though the transcription had been considered to be highly repressed so far. Moreover, we revealed that the activation of the newly inserted retrotransposons is maintained at least for several generations of the life cycle of zebrafish. These data provide a new insight in the field of retrotransposon study.

Report

(6 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (8 results)

All 2015 2014 2013 2012 2011

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (1 results)

  • [Journal Article] LINE retrotransposition and host DNA repair machinery.2015

    • Author(s)
      Yamaguchi K, Kajikawa M, Okada N.
    • Journal Title

      Mob Genet Elements.

      Volume: 5 Issue: 6 Pages: 92-97

    • DOI

      10.1080/2159256x.2015.1096998

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Integrated mechanism for the generation of the 5' junctions of LINE inserts.2014

    • Author(s)
      Yamaguchi K., Kajikawa M. and Okada N.
    • Journal Title

      Nucleic Acids Research

      Volume: 42 Issue: 21 Pages: 13269-13279

    • DOI

      10.1093/nar/gku1067

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Mechanism by which a LINE protein recognizes its 3' tail RNA.2014

    • Author(s)
      Hayashi Y., Kajikawa M., Matsumoto T. and Okada N.
    • Journal Title

      Nucleic Acids Research

      Volume: 42 Issue: 16 Pages: 10605-10617

    • DOI

      10.1093/nar/gku753

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] In vitro screening for compounds that enhance human L1 mobilization2013

    • Author(s)
      Terasaki N, Goodier JL, Cheung LE, Wang YJ, Kajikawa M, Kazazian HH Jr, Okada N.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 9 Pages: e74629-e74629

    • DOI

      10.1371/journal.pone.0074629

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new mechanism to ensure integration during LINE retrotransposition: a suggestion from analyses of the 5' extra nucleotides.2012

    • Author(s)
      Kajikawa M, Yamaguchi K, Okada N.
    • Journal Title

      Gene

      Volume: 505 Issue: 2 Pages: 345-351

    • DOI

      10.1016/j.gene.2012.02.047

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low dependency of retrotransposition on the ORF1 protein of the zebrafish LINE, ZfL2-1.2012

    • Author(s)
      Kajikawa M, Sugano T, Sakurai R, Okada N.
    • Journal Title

      Gene

      Volume: 499 Issue: 1 Pages: 41-47

    • DOI

      10.1016/j.gene.2012.02.048

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Self-interaction, nucleic acid binding, and nucleic acid chaperone activities are unexpectedly retained in the unique ORF1p of zebrafish LINE2012

    • Author(s)
      Mitsuhiro Nakamura
    • Journal Title

      Mol.Cell.Biol.

      Volume: 32 Pages: 458-469

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] The zebrafish LINE, ZfL2-1, has a potential to obtain a novel coding sequence in its ORF1p coding region2011

    • Author(s)
      Masaki Kajikawa
    • Organizer
      Masaki Kajikawa
    • Place of Presentation
      Snowmass Village, Colorado, USA
    • Year and Date
      2011-08-10
    • Related Report
      2011 Annual Research Report

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Published: 2011-08-01   Modified: 2019-07-29  

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