| Budget Amount *help |
¥122,330,000 (Direct Cost: ¥94,100,000、Indirect Cost: ¥28,230,000)
Fiscal Year 2015: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2014: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2013: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
Fiscal Year 2012: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2011: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
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| Outline of Final Research Achievements |
Genomic integrity is constantly challenged by damage from a variety of sources such as certain chemical agents and UV radiation. The repetitive sequences and chromatin compaction characteristic of non-coding DNA are known to constitute a barrier for DNA replication and repair.Hence, DNA damage and replication stress caused in non-coding DNA regions are implicated in genomic instability. Yeast Srs2, conserved from bacteria to humans, plays important roles in the integrity of repetitive sequences by regulating homologous recombination (HR) pathway. In this study, we demonstrated that Srs2 participates in essential pathways preventing accumulation of inter-homolog recombination intermediates, thereby reducing the risk of genome instability. This work also identified novel H3/H4 alleles involved in replication stress tolerance, which requires a functional HR pathway. Thus, our data identify novel roles of Srs2 and histone H3/H4 in regulating the HR pathway.
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