| Budget Amount *help |
¥122,330,000 (Direct Cost: ¥94,100,000、Indirect Cost: ¥28,230,000)
Fiscal Year 2015: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2014: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2013: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
Fiscal Year 2012: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2011: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
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| Outline of Final Research Achievements |
We examined chromatin assembly network at centromeres by synthetically generating functional structures using synthetic repetitive DNAs and combination of various fusion protein tetherings. Results indicated that CENP-C and CENP-I are key connecting factors for the functional kinetochore assembly and an epigenetic CENP-A chromatin replenishment. Further, we identified the interaction between M18BP1, a CENP-A assembly factor just downstream of CENP-C, and acetyltransferase KAT7/HBO1/MYST2. Knocking out KAT7 in HeLa cells reduced centromeric CENP-A levels. Acetylation of histone H3K14 induced by KAT7 provides competence for histone turnover/exchange activity and prevents Suv39h1-mediated heterochromatin invasion into centromeres. And thus, this reaction promotes HJURP mediated CENP-A replenishment on the repetitive DNA.
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