Development of novel methods for single cell quantification with microdevices

• Project Area: Spying minority in biological phenomena -Toward bridging dynamics between individual and ensemble processes-
• Project/Area Number: 23115002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: Noji Hiroyuki 東京大学, 工学(系)研究科(研究院), 教授 (00343111)
• Co-Investigator(Kenkyū-buntansha): WATANABE Tomonobu 国立研究開発法人理化学研究所, 生命システム研究センター, チームリーダー (00375205) ; ICHIMURA Taro 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (50600748)
• Co-Investigator(Renkei-kenkyūsha): FUJITA Katsumasa 大阪大学, 工学研究科, 准教授 (80362664)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥187,460,000 (Direct Cost: ¥144,200,000、Indirect Cost: ¥43,260,000); Fiscal Year 2015: ¥33,410,000 (Direct Cost: ¥25,700,000、Indirect Cost: ¥7,710,000); Fiscal Year 2014: ¥33,410,000 (Direct Cost: ¥25,700,000、Indirect Cost: ¥7,710,000); Fiscal Year 2013: ¥35,230,000 (Direct Cost: ¥27,100,000、Indirect Cost: ¥8,130,000); Fiscal Year 2012: ¥34,840,000 (Direct Cost: ¥26,800,000、Indirect Cost: ¥8,040,000); Fiscal Year 2011: ¥50,570,000 (Direct Cost: ¥38,900,000、Indirect Cost: ¥11,670,000)
• Keywords: 1分子生物物理 / マイクロデバイス / 超解像顕微鏡 / 蛍光プローブ / 1細胞解析 / デジタルバイオ分析 / 1分子イメージング / マイクロドロプレットアレイ / ATP / インフルエンザ / 蛍光タンパク質 / デジタル計測 / 1分子計測 / ウイルス計測 / ELISA / 超解像 / 1分子検出

Outline of Final Research Achievements

Noji group in the University of Tokyo aimed to develop novel digital counting quantification methods with single-molecule or single-particle sensitivity by use of femtoliter chamber array device. Digital ELISA with unprecedentedly high sensitivity was established and applied for counting of absolute numbers of protein molecules expressed in single cells. Digital Virus counting method was also developed that revealed the only a few % of total virus population possess the capability of infection. Watanabe group in Riken developed novel imaging methods for protein quantification such as multicolor single-molecule imaging method and fundamental intracellular parameters that represent whole cellular activities such as total protein abundance and gross protein diffusion coefficient.

Research Products

• [Journal Article] Novel nano-device to measure voltage-driven membrane transporter activity (2016)
  • Author(s): Watanabe, R., Soga, N., & Noji, H.
  • Journal Title: IEEE Transactions on Nanotechnology Volume: 15 Issue: 1 Pages: 70-73
  • DOI: 10.1109/tnano.2015.2498167
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. (2016)
  • Author(s): Takamitsu J. Morikawa, Hideaki Fujita, Akira Kitamura, Takashi Horio, Johtaro Yamamoto, Masataka Kinjo, Akira Sasaki, Hiroaki Machiyama, Keiko Yoshizawa, Taro Ichimura, Katsumi Imada, Takeharu Nagai & Tomonobu M. Watanabe
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 22342-22342
  • DOI: 10.1038/srep22342
  • Peer Reviewed / Open Access
• [Journal Article] ラマン散乱スペクトルによる細胞指紋法の提案 (2016)
  • Author(s): 渡邉朋信, 市村垂生, 藤田英明
  • Journal Title: BIO INDUSTRY Volume: 2 Pages: 19-25
• [Journal Article] Full control of polarization state with a pair of electro-optic modulators for polarization-resolved optical microscopy (2016)
  • Author(s): Junichi Kaneshiro, Tomonobu. M. Watanabe, Hideaki Fujita, Taro Ichimura
  • Journal Title: Applied Optics Volume: 55 Issue: 5 Pages: 1082-1089
  • DOI: 10.1364/ao.55.001082
  • Peer Reviewed / Open Access
• [Journal Article] A single-molecule digital enzyme assay using alkaline phosphatase with a cumarin-based fluorogenic substrate (2015)
  • Author(s): Obayashi Y, Iino R, Noji H
  • Journal Title: Analyst Volume: 140 Issue: 15 Pages: 5065-6073
  • DOI: 10.1039/c5an00714c
  • Peer Reviewed / Open Access
• [Journal Article] Giant Acceleration of diffusion observed in a single-molecule experiment on F1-ATPase (2015)
  • Author(s): Hayashi R, Sasaki K, Nakamura S, Kudo S, Inoue Y, Noji H, Hayashi K
  • Journal Title: Physical Review letters Volume: 114
  • Peer Reviewed / Open Access
• [Journal Article] Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters (2015)
  • Author(s): Soga, N., *Watanabe, R., & Noji, H.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11025-11025
  • DOI: 10.1038/srep11025
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry (2015)
  • Author(s): Hideaki Fujita, Tsuyoshi Esaki, Tsutomu Masujima, Akitsu Hotta, Soo Hyeon Kim, Hiroyuki Noji, Tomonobu M. Watanabe
  • Journal Title: RSC Adv. Volume: 5 Issue: 22 Pages: 16968-16971
  • DOI: 10.1039/c4ra12021c
  • Peer Reviewed / Open Access
• [Journal Article] Effects of an ATP analogue, adenosine 5′-[α-thio]-triphosphate, on F1-ATPase rotary catalysis, torque generation, and inhibited intermediated formation (2015)
  • Author(s): Yukawa A, Watanabe R and Noji H
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 458 Issue: 3 Pages: 515-519
  • DOI: 10.1016/j.bbrc.2015.01.146
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] High-speed angle-resolved imaging of single gold nanorod with microsecond temporal resolution and one degree angle precision (2015)
  • Author(s): S. Enoki, R. Iino, Y. Niitani, Y. Minagawa, M. Tomishige, H. Noji
  • Journal Title: Anal. Chem. Volume: 87 Issue: 4 Pages: 2079-2086
  • DOI: 10.1021/ac502408c
  • Peer Reviewed / Open Access
• [Journal Article] Torque transmission mechanism via DELSEED loop of F1-ATPase (2015)
  • Author(s): Watanabe R, Koyasu K, You H, Tanigawara M and Noji H
  • Journal Title: Biophysical Journal Volume: 108 Issue: 5 Pages: 1144-1152
  • DOI: 10.1016/j.bpj.2015.01.017
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Predictive diagnosis of the risk of breast cancer recurrence after surgery by single-particle quantum dot imaging. (2015)
  • Author(s): Gonda K, Miyashita M, Higuchi H, Tada H, Watanabe TM, Watanabe M, Ishida T, Ohuchi N.
  • Journal Title: Scientific Report. Volume: 22(5) Issue: 1 Pages: 14322-14322
  • DOI: 10.1038/srep14322
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] SH3 domain of c-Src governs its dynamics at focal adhesions and the cell membrane (2015)
  • Author(s): Machiyama H, Yamaguchi T, Sawada Y, Watanabe T, Fujita H
  • Journal Title: FEBS Journal Volume: 282 Issue: 20 Pages: 4034-4055
  • DOI: 10.1111/febs.13404
  • Peer Reviewed
• [Journal Article] Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging. (2015)
  • Author(s): Taro Ichimura, Liang-da Chiu, Katsumasa Fujita, Hiroaki Machiyama, Satoshi Kawata, Tomonobu M. Watanabe, Hideaki Fujita
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11358-11358
  • DOI: 10.1038/srep11358
  • Peer Reviewed / Open Access
• [Journal Article] 局在表面プラズモンを利用した超解像顕微分光とイメージング (2015)
  • Author(s): 市村垂生、矢野隆章
  • Journal Title: 分光研究 Volume: 64 Pages: 582-598
  • NAID: 40020722646
  • Peer Reviewed
• [Journal Article] 細胞状態を示す指紋 細胞からのラマンスペクトル (2015)
  • Author(s): 藤田英明、市村垂生、渡邉朋信
  • Journal Title: 現代化学 Volume: 11 Pages: 36-40
  • NAID: 40020613388
  • Peer Reviewed
• [Journal Article] Measurement of Cellular State by Raman Microscopy (2014)
  • Author(s): 市村垂生
  • Journal Title: Seibutsu Butsuri Volume: 54 Issue: 6 Pages: 315-317
  • DOI: 10.2142/biophys.54.315
  • NAID: 130004713032
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access
• [Journal Article] Nano-scale measurement of biomolecules by optical microscopy and semiconductor nanoparticles (2014)
  • Author(s): Taro Ichimura, Takashi Jin, Hideaki Fujita, Hideo Higuchi, Tomonobu M. Watanabe
  • Journal Title: Front. Physiol., fphys. Volume: 5 Pages: 1-13
  • DOI: 10.3389/fphys.2014.00273
  • Peer Reviewed / Open Access
• [Journal Article] Gene dynamics of core transcription factors for pluripotency in embryonic stem cells (2014)
  • Author(s): Brit G. David, Kazuko Okamoto, Taishi Kakizuka, Taro Ichimura, Tomonobu M. Watanabe, Hideaki Fujita
  • Journal Title: J. Biosci. Bioeng Volume: 119 Issue: 4 Pages: 406-409
  • DOI: 10.1016/j.jbiosc.2014.09.011
  • NAID: 110009942819
  • Peer Reviewed / Open Access
• [Journal Article] Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers (2014)
  • Author(s): Sayaka Higuchi, Tomonobu M. Watanabe, Keiko Kawauchi, Taro Ichimura, Hideaki Fujita
  • Journal Title: J Biosci Bioeng Volume: 117 Issue: 6 Pages: 749-75
  • DOI: 10.1016/j.jbiosc.2013.11.011
  • NAID: 110009823162
  • Peer Reviewed / Open Access
• [Journal Article] Single-molecule Imaging Analysis of Elementary Reaction Steps of Trichoderma reesei Cellobiohydrolase I (Cel7A) Hydrolyzing Crystalline Cellulose Iα and IIII (2014)
  • Author(s): Y.Shibafuji, A.Nakamura, T.Uchihashi, N.Sugimoto, S.Fukuda, H.Watanabe, M.Samejima, T.Ando, H.Noji, A.Koivula, K.Igarashi, R.Iino
  • Journal Title: Journal of Biological Chemistry Volume: 289 (29) Issue: 20 Pages: 1405614065-1405614065
  • DOI: 10.1074/jbc.m113.546085
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of the temperature-sensitive reaction of F1-ATPase by using single molecule manipulation (2014)
  • Author(s): Watanabe R and Noji H
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 4962-4962
  • DOI: 10.1038/srep04962
  • Peer Reviewed / Open Access
• [Journal Article] Timing of inorganic phosphate release modulates the catalytic activity of ATP-driven rotary motor protein (2014)
  • Author(s): Watanabe R and Noji H
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 3486-3486
  • DOI: 10.1038/ncomms4486
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] F1subunit reinforces torque generation in V-ATPase (2014)
  • Author(s): Junichi Kishikawa, Akihiko Seino, Atsuko Nakanishi, Naciye Esma Tirtom, Hiroyuki Noji, Ken Yokoyama, Kumiko Hayashi
  • Journal Title: European Biophysics Journal Volume: 43 Issue: 8-9 Pages: 415-422
  • DOI: 10.1007/s00249-014-0973-x
  • Peer Reviewed / Open Access
• [Journal Article] Torque generation mechanism of F1-ATPase upon NTP binding (2014)
  • Author(s): Arai HC, Yukawa A, Iwatate JR, Kamiya M, Watanabe R, Urano Y and Noji H
  • Journal Title: Biophys J Volume: 107 Issue: 1 Pages: 156-164
  • DOI: 10.1016/j.bpj.2014.05.016
  • Peer Reviewed / Open Access
• [Journal Article] Real-Time Fluorescence Visualization of Slow Tautomerization of Single Free-Base Phthalocyanines under Ambient Conditions (2014)
  • Author(s): Ikeda T, Iino R and Noji H
  • Journal Title: Chem. Commun Volume: 50 Issue: 67 Pages: 9443-9446
  • DOI: 10.1039/c4cc02574a
  • Peer Reviewed / Open Access
• [Journal Article] Motion Capture and Manipulation of Single Synthetic Molecular Rotors by Optical Microscopy (2014)
  • Author(s): Ikeda T, Tsukahara T, Iino R, Takeuchi M and Noji H
  • Journal Title: Angew.Chem.Int.Ed Volume: 53 Issue: 38 Pages: 10082-10085
  • DOI: 10.1002/ange.201403091
  • Peer Reviewed / Open Access
• [Journal Article] Robustness of the Rotary Catalysis Mechanism of F1-ATPase (2014)
  • Author(s): Watanabe R, Matsukage Y, Yukawa A, Tabata KV and Noji H
  • Journal Title: J Biol Chem Volume: 289 Issue: 28 Pages: 19331-19340
  • DOI: 10.1074/jbc.m114.569905
  • Peer Reviewed / Open Access
• [Journal Article] Arrayed lipid bilayer chambers allow single-molecule analysis of membrane transporter activity (2014)
  • Author(s): Watanabe R., Soga N., Fujita D., Tabata K. V., Yamauchi L., Kim S. H., Asanuma D., Kamiya M., Urano Y., Suga H. & Noji H.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 4519-4519
  • DOI: 10.1038/ncomms5519
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging (2014)
  • Author(s): Hideyuki Yaginuma, Shinnosuke Kawai, Kazuhito V. Tabata, Keisuke Tomiyama, Akira Kakizuka, Tamiki Komatsuzaki, Hiroyuki Noji, and Hiromi Imamura
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 6522-6522
  • DOI: 10.1038/srep06522
  • NAID: 120005575159
  • Peer Reviewed / Open Access
• [Journal Article] Thermodynamic analysis of F1-ATPase rotary catalysis using high-speed imaging (2014)
  • Author(s): Watanabe R, Minagawa Y and Noji H
  • Journal Title: Protein Science Volume: 23 Issue: 12 Pages: 1773-1779
  • DOI: 10.1002/pro.2559
  • Peer Reviewed / Open Access
• [Journal Article] Torque generation of Enterococcus hirae V-ATPase (2014)
  • Author(s): Ueno H, Minagawa Y, Hara M, Rahman S, Yamato I, Muneyuki E, Murata T, and Iino R
  • Journal Title: The Journal of Biological Chemistry Volume: 289 Issue: 45 Pages: 31212-31223
  • DOI: 10.1074/jbc.m114.598177
  • Peer Reviewed / Open Access
• [Journal Article] High-throughput formation of lipid bilayer membrane arrays with an asymmetric lipid composition (2014)
  • Author(s): Watanabe R., Soga N., Yamanaka T. & Noji H.
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 7076-7076
  • DOI: 10.1038/srep07076
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Key Chemical Factors of Arginine Finger Catalysis of F1-ATPase Clarified by an Unnatural Amino Acid Mutation (2014)
  • Author(s): Yukawa A, Iino R, Watanabe R, Hayashi S and Noji H
  • Journal Title: Biochemistry Volume: 54 Issue: 2 Pages: 472-480
  • DOI: 10.1021/bi501138b
  • Peer Reviewed / Open Access
• [Journal Article] Catalysis-enhancement via rotary fluctuation of F1-ATPase (2013)
  • Author(s): Watanabe R, Hayashi K, Ueno H and Noji H
  • Journal Title: Biophys. J Volume: 105 Issue: 10 Pages: 2385-2391
  • DOI: 10.1016/j.bpj.2013.09.050
  • Peer Reviewed
• [Journal Article] Glucose-stimulated single pancreatic islets sustain increased cytosolic ATP levels during initial Ca2+ influx and subsequent Ca2+ oscillations (2013)
  • Author(s): Tanaka T, Nagashima K, Inagaki N, Kioka H, Takashima S, Fukuoka H, Noji H, Kakizuka A and Imamura H
  • Journal Title: J Biol Chem Volume: 289 Issue: 4 Pages: 2205-2216
  • DOI: 10.1074/jbc.m113.499111
  • NAID: 120005689188
  • Peer Reviewed
• [Journal Article] Quantifying genetically inserted fluorescent protein in single iPS cells to monitor Nanog expression using electroactive microchamber arrays (2013)
  • Author(s): Kim SH, He X, Kaneda S, Kawada J, Fourmy D, Noji H and Fujii T
  • Journal Title: Lab on a Chip Volume: 14 Issue: 4 Pages: 730-736
  • DOI: 10.1039/c3lc51086g
  • Peer Reviewed
• [Journal Article] Evaluation of intra-mitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation (2013)
  • Author(s): Kioka H, Kato H, Fujikawa M, Tsukamoto O, Suzuki T, Imamura H, Nakano A, Higo S, Yamazaki S, Matsuzaki T, Tkafuji K, Asanuma H, Asakura M, Minamino T, Shintani Y, Yoshida M, Noji H, Kitakaze M, Komuro I, Asano Y and Takashima S
  • Journal Title: PNAS Volume: 111 Issue: 1 Pages: 273-278
  • DOI: 10.1073/pnas.1318547111
  • Peer Reviewed / Open Access
• [Journal Article] Basic properties of rotary dynamics of the molecular motor Enterococcus hirae V1-ATPase (2013)
  • Author(s): Yoshihiro Minagawa, Hiroshi Ueno, Mayu Hara, Yoshiko Ishizuka-Katsura, Noboru Ohsawa,Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama, Ichiro Yamato, Eiro Muneyuki, Hiroyuki Noji, Takeshi Murata, and Ryota Iino
  • Journal Title: J.Biol.Chem Volume: 288 Issue: 45 Pages: 32700-32707
  • DOI: 10.1074/jbc.m113.506329
  • Peer Reviewed
• [Journal Article] Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force (2013)
  • Author(s): Watanabe R, Tabata KV, Iino R, Ueno H, Iwamoto M, Oiki S, Noji H
  • Journal Title: Nature Communications Volume: 4 Issue: 1 Pages: 1-6
  • DOI: 10.1038/ncomms2631
  • Peer Reviewed
• [Journal Article] In vivo fluorescent ATP imaging of Drosophila melanogaster and Caenorhabditis elegans by using a genetically encoded fluorescent ATP biosensor optimized for low temperatures. (2013)
  • Author(s): Tsuyama, Taiichi; Kishikawa, Jun-ichi; Han, Yong-Woon; Harada, Yoshie; Tsubouchi, Asako; Noji, Hiroyuki; Kakizuka, Akira; Yokoyama, Ken; Uemura, Tadashi; Imamura, Hiromi.
  • Journal Title: Analytical Chemistry Volume: 85(16) Issue: 16 Pages: 7889-7896
  • DOI: 10.1021/ac4015325
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Single-molecule analysis of FoF1-ATP synthase inhibited by N,N-dicyclohexylcarbodiimide (2013)
  • Author(s): Toei M and Noji H
  • Journal Title: J Biol Chem Volume: 288 Issue: 36 Pages: 25717-25726
  • DOI: 10.1074/jbc.m113.482455
  • Peer Reviewed
• [Journal Article] Single molecule analysis of the rotation of F1-ATPase under high hydrostatic pressure (2013)
  • Author(s): Daichi Okuno, Masayoshi Nishiyama and Hiroyuki Noji
  • Journal Title: Biophysical Journal Volume: 105 Issue: 7 Pages: 1635-1642
  • DOI: 10.1016/j.bpj.2013.08.036
  • NAID: 120005328380
  • Peer Reviewed
• [Journal Article] Label-free single-particle imaging of the influenza virus by objective-type total internal reflection dark-field microscopy (2012)
  • Author(s): Enoki S
  • Journal Title: PLoS One Volume: 7(11) Issue: 11 Pages: e49208-e49208
  • DOI: 10.1371/journal.pone.0049208
  • Peer Reviewed
• [Journal Article] Hatsugai N, Koldenkova VP, Imamura H, Noji H, Nagai T (2012)
  • Author(s): Hatsugai N, Koldenkova VP, Imamura H, Noji H, Nagai T
  • Journal Title: Plant. Cell Physiol Volume: 53 Issue: 10 Pages: 1768-1775
  • DOI: 10.1093/pcp/pcs119
  • Peer Reviewed
• [Journal Article] Large-scale femtoliter droplet array for digital counting of single biomolecules (2012)
  • Author(s): Kim SH
  • Journal Title: Lab on a Chip Volume: 12 Issue: 23 Pages: 4986-4991
  • DOI: 10.1039/c2lc40632b
  • Peer Reviewed
• [Journal Article] A single-cell drug efflux assay in bacteria by using a directly accessible femtoliter droplet array (2012)
  • Author(s): Iino R
  • Journal Title: Lab on a Chip Volume: 12 Issue: 20 Pages: 3923-3929
  • DOI: 10.1039/c2lc40394c
  • Peer Reviewed
• [Presentation] 散乱光を用いた生体計測 (2016)
  • Author(s): 渡邉朋信
  • Organizer: レーザー学会第36回年次大会
  • Place of Presentation: 名城大学（愛知県名古屋市）
  • Year and Date: 2016-01-10
  • Invited
• [Presentation] Single molecule analysis of transporter protein with arrayed lipid bilayer chamber (2015)
  • Author(s): Hiroyuki Noji
  • Organizer: Pacific Chem
  • Place of Presentation: Hawaii Convention Center (Honolulu, Hawaii, US)
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] Redesigning of F1-ATPase (2015)
  • Author(s): Hiroyuki Noji
  • Organizer: Pacific Chem
  • Place of Presentation: Hawaii Convention Center (Honolulu, Hawaii, US)
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] Single cell Non-invasive spectroscopy (2015)
  • Author(s): Tomonobu M Watanabe
  • Organizer: 3rd International Symposium on Nanomedicine Molecular Science
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-11-26
  • Int'l Joint Research / Invited
• [Presentation] Single molecule biophysics of ATP synthase and digitalization revolution of bioassay (2015)
  • Author(s): Hiroyuki Noji
  • Organizer: POSTECH seminar
  • Place of Presentation: Pohang University of Science and Technology, (Pohang-si, Korea)
  • Year and Date: 2015-09-24
  • Int'l Joint Research / Invited
• [Presentation] Development of imaging technology to detect intracellular physical parameters (2015)
  • Author(s): Tomonobu M Watanabe
  • Organizer: THE HONG KONG UNIVERSITY OF SCIENCE & TECHNOLOGY Joint Seminar
  • Place of Presentation: The HK University of Science & Technology(中国、香港）
  • Year and Date: 2015-09-24
  • Int'l Joint Research
• [Presentation] 波長分離による複数分子ナノ追跡 (2015)
  • Author(s): 市村垂生、垣塚太志、池崎圭吾、金城純一、藤田英明、渡邉朋信
  • Organizer: 第５６回応用物理学会年会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-09-13
• [Presentation] 『単一』にこだわる計測技術開発 (2015)
  • Author(s): 渡邉朋信
  • Organizer: 新学術領域 超高速バイオアセンブラ第 4 回若手シンポジウム
  • Place of Presentation: 大阪大学（大阪府豊中市）
  • Year and Date: 2015-07-03
  • Invited
• [Presentation] 細胞と細胞集団との多層にまたがる状態遷移の定量解析 (2015)
  • Author(s): 渡邉朋信
  • Organizer: 第67回日本細胞生物学会大会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2015-07-01
• [Presentation] 蛍光ゆらぎ相関計算に基づく超解像法 (2015)
  • Author(s): 渡邉朋信
  • Organizer: 日本顕微鏡学会第71回学術講演会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2015-05-15
  • Invited
• [Presentation] ラマン散乱顕微鏡による細胞状態遷移の一細胞計測 (2015)
  • Author(s): 市村垂生、Liang-da Chiu、藤田克昌、渡邉朋信、藤田英明
  • Organizer: 日本顕微鏡学会第７１回学術講演会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2015-05-13
• [Presentation] Single-molecule biophysics on ATP synthase (2014)
  • Author(s): Hiroyuki Noji
  • Organizer: 2014 International Biophysics Congress(IUPAB2014)
  • Place of Presentation: Brisbane Convention & Exhibition Centre(Brisbane, Australia)
  • Year and Date: 2014-08-07
  • Invited
• [Presentation] Arrayed Lipid Bilayer Chamber for single-molecule analysis of transporters (2014)
  • Author(s): Hiroyuki Noji
  • Organizer: European Bioenergetics Conference(EBEC2014)
  • Place of Presentation: Faculdade de Ciencias(Lisbon, Portugal)
  • Year and Date: 2014-07-13
  • Invited
• [Presentation] Single-Molecule Digital ELISA and Prospects of its Applications (2014)
  • Author(s): Hiroyuki Noji
  • Organizer: World Lecture Series on Micro/Nanofluidics
  • Place of Presentation: Keio University(Kanagawa, Japan)
  • Year and Date: 2014-07-02
  • Invited
• [Presentation] Torque generation mechanism of F1-ATPase (2014)
  • Author(s): Hiroyuki Noji
  • Organizer: Tokyo ATPase Workshop(TAW)
  • Place of Presentation: The University of Tokyo (Tokyo, Japan)
  • Year and Date: 2014-06-02
  • Invited
• [Presentation] Design Principle of F1‐ATPase Rotary Molecular Motor (2013)
  • Author(s): Hiroyuki Noji
  • Organizer: 日仏セミナー
  • Place of Presentation: 在日フランス大使館（東京都）
  • Year and Date: 2013-02-06
  • Invited
• [Presentation] Single-molecule digital counting of proteins (2012)
  • Author(s): Hiroyuki Noji
  • Organizer: Paradigm Innovation in Biology: Novel Strategy and Thinking
  • Place of Presentation: 中央科学院（Taipei） (Taiwan)
  • Year and Date: 2012-10-17
  • Invited
• [Presentation] Single-Molecule Digital Bioassay with a Million Droplets Array (2012)
  • Author(s): Hiroyuki Noji
  • Organizer: International Conference Session 5
  • Place of Presentation: 幕張メッセ（千葉県）
  • Year and Date: 2012-09-07
  • Invited
• [Presentation] Single-Molecule ELISA (2011)
  • Author(s): Hiroyuki Noji
  • Organizer: 日本分子細胞生物学会
  • Place of Presentation: パシフィコ横浜(招待講演)
• [Presentation] Recent advances of Single molecule biophysics of ATP synthase
  • Author(s): Hiroyuki Noji
  • Organizer: The 8th International Conference on Advanced Mateials and Device
  • Place of Presentation: Korea, Jeju, Ramada Plaza Jeju Hotel
  • Invited
• [Presentation] Novel single-molecule systems to monitor the rotary dynamics and proton-pumping of FoF1-ATP synthase
  • Author(s): Hiroyuki Noji
  • Organizer: ITALY IN JAPAN 2013 WORKSHOP (Methods for the investigation of ion transport machinery in biological Membranes)
  • Place of Presentation: 東京都、イタリア文化会館
  • Invited
• [Presentation] Single-molecule digital counting with femtoliter chamber array
  • Author(s): Hiroyuki Noji
  • Organizer: International Symposium on Nanomedicine Molecular Science 2013
  • Place of Presentation: 東京都、東京大学
  • Invited
• [Presentation] Torque-generation mechanism of F1-ATPase
  • Author(s): Hiroyuki Noji
  • Organizer: The 13th KIAS Conference on Protein Structure and Function
  • Place of Presentation: Korea、Seoul、Korea Institute for Advanced Study
  • Invited
• [Presentation] Single-Molecule counting of biomolecules with femtoliter dropret chamber array
  • Author(s): Hiroyuki Noji
  • Organizer: The 17th International Conference on Solid-State Sensors
  • Place of Presentation: Spain, Barcelona, Barcelona International Convention Centre
  • Invited
• [Presentation] Single molecule biophysics of ATP synthase
  • Author(s): Hiroyuki Noji
  • Organizer: Single Molecule Workshop
  • Place of Presentation: Taiwan, Taipei, National Taicung University
  • Invited
• [Presentation] Single-Molecule Technology
  • Author(s): Hiroyuki Noji
  • Organizer: Single-Molecule Biophysical Chemistry Workshop
  • Place of Presentation: Taiwan, Taipei, National Taiwan University
  • Invited
• [Presentation] Single-Molecule Biophysics of ATP synthase
  • Author(s): Hiroyuki Noji
  • Organizer: Single-Molecule Biophysical Chemistry Workshop
  • Place of Presentation: Taiwan, Taipei, National Taiwan University
  • Invited
• [Presentation] Femtoliter chamber array for digital ELISA and single cell analysis
  • Author(s): Hiroyuki Noji
  • Organizer: Workshop on Technologies for Single Cell Analysis
  • Place of Presentation: 東京都、Hitachi Research Institute
  • Invited
• [Patent(Industrial Property Rights)] 高密度微小チャンバーアレイおよびその製造方法 (2013)
  • Inventor(s): 野地博行, 渡邉力也, 菅裕明, 藤田大士
  • Industrial Property Rights Holder: 野地博行, 渡邉力也, 菅裕明, 藤田大士
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-171493
  • Filing Date: 2013-08-21
• [Patent(Industrial Property Rights)] 検出装置及び検出方法 (2013)
  • Inventor(s): 野地博行、金秀炫、飯野亮太、太田淳、徳田崇、笹川清隆、野田俊彦
  • Industrial Property Rights Holder: 野地博行、金秀炫、飯野亮太、太田淳、徳田崇、笹川清隆、野田俊彦
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-08-29
  • Overseas