| Co-Investigator(Renkei-kenkyūsha) |
SANDO SHINSUKE 東京大学, 工学研究科, 教授 (20346084)
URANO YASUTERU 東京大学, 薬学研究科, 教授 (20292956)
OZAWA TAKEAKI 大阪大学, 理学研究科, 教授 (40302806)
MATSUDA TOMOKI 大阪大学, 産業科学研究所, 准教授 (50419206)
ARAI YOSHIYUKI 大阪大学, 産業科学研究所, 助教 (20444515)
|
|---|
| Budget Amount *help |
¥288,470,000 (Direct Cost: ¥221,900,000、Indirect Cost: ¥66,570,000)
Fiscal Year 2015: ¥54,340,000 (Direct Cost: ¥41,800,000、Indirect Cost: ¥12,540,000)
Fiscal Year 2014: ¥54,340,000 (Direct Cost: ¥41,800,000、Indirect Cost: ¥12,540,000)
Fiscal Year 2013: ¥57,590,000 (Direct Cost: ¥44,300,000、Indirect Cost: ¥13,290,000)
Fiscal Year 2012: ¥56,940,000 (Direct Cost: ¥43,800,000、Indirect Cost: ¥13,140,000)
Fiscal Year 2011: ¥65,260,000 (Direct Cost: ¥50,200,000、Indirect Cost: ¥15,060,000)
|
|---|
| Outline of Final Research Achievements |
We developed a wide variety of tools to visualize physiological events caused by small number of biomolecules, and also to manipulate molecular number inside cells with light. Included are photo-switchable fluorescent proteins, fluorescent/bioluminescent biosensor proteins, photosensitizer proteins, caged amino acids, ligand-responsive ion channel, microscope with a device for patterned illumination and cavity-reflection-enhanced absorption microscope. We supported researches in other groups by applying these technologies. Moreover, with the developed tools we approached the molecular mechanism of chemotaxis in D. discoidium, and discovered biomolecules that can function at very low concentrations (several tens molecules/um^2). We also found that very small population of cells among thousands of cell have a predominant role in self-organizing aggregation pattern formation.
|
