| Budget Amount *help |
¥90,740,000 (Direct Cost: ¥69,800,000、Indirect Cost: ¥20,940,000)
Fiscal Year 2015: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2014: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2013: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2012: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2011: ¥28,600,000 (Direct Cost: ¥22,000,000、Indirect Cost: ¥6,600,000)
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| Outline of Final Research Achievements |
How are the two copies of genomic DNA organized and read out in the cell? Although the chromatin had been long assumed to be folded into the 30-nm chromatin fiber and further helical folding structures, we demonstrated that chromatin essentially irregularly folded without the chromatin fiber. Consistent with this notion, using single nucleosome imaging, we uncovered local nucleosome fluctuation in live mammalian cells. Our in vivo/in silico results suggested that local nucleosome dynamics facilitate chromatin accessibility and play an important role in the scanning of genome information, which is critical for many “target searching” biological processes.
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