| Budget Amount *help |
¥92,170,000 (Direct Cost: ¥70,900,000、Indirect Cost: ¥21,270,000)
Fiscal Year 2015: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2014: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2013: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2012: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2011: ¥28,600,000 (Direct Cost: ¥22,000,000、Indirect Cost: ¥6,600,000)
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| Outline of Final Research Achievements |
Previous studies suggest that the expression level of circadian core factors, most of them work as transcription regulators are generally low. In this project, we carried out absolute quantification of circadian core transcription factors throughout the various circadian time points, and revealed that the expression amount varies between 1,000 ~ 20,000 molecules / cell, the concentration range lower than the expression level of most proteins in cell. We then asked the mechanism, by which circadian transcription factors at low expression level ensures rhythmic transcription oscillation with precise 24 hour period. We found that CRY, a core component of circadian transcription factors regulates circadian period through amount regulation depending on the turnover rate of CRY protein and state regulation depending on the phosphorylation status of the protein.
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