| Budget Amount *help |
¥137,670,000 (Direct Cost: ¥105,900,000、Indirect Cost: ¥31,770,000)
Fiscal Year 2015: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2014: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2013: ¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
Fiscal Year 2012: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
Fiscal Year 2011: ¥41,730,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥9,630,000)
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| Outline of Final Research Achievements |
To understand the molecular mechanism of flagellar construction, we investigated the flagellar protein export system that is driven and regulated by the turnover of multifunctional component proteins, each of which functions in limited numbers. We established an in-vitro assay system using inverted membrane vesicle that enables precise control of the external condition. The system retains the secretion activity as in the cell. Using this system together with in vivo single molecule experiment and biochemical and genetic techniques, we revealed a novel role of the export ATPase proteins, the energy source for secretion and the mechanism of ordered secretion. We also found that export chaperone proteins change their structure to control the binding affinity for their interaction partners and thereby regulate the secretion order. In addition, we developed the high speed AFM and visualized the structural change and assembly dynamics of protein complexes.
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