|Budget Amount *help
¥91,260,000 (Direct Cost: ¥70,200,000、Indirect Cost: ¥21,060,000)
Fiscal Year 2015: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2014: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2013: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2012: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2011: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
|Outline of Final Research Achievements
A green ciliate Paramecium bursaria harbors several hundred Chlorella cells in its cytoplasm. An electron microscopic approach with 3-D reconstruction showed that mitochondria in the host's cytoplasm provide a structural scaffold for symbiotic Chlorella to be anchored in the host's cytoplasm. Proteome and de novo transcriptome sequencing analyses were carried out to identify proteins in the peri-algal membrane fraction. As a distinctive aspect of the unicellular Matryoshka-type protists, P. bursaria was also found to accumulate soil-bound radioactive cesium.
Anaerobic protozoan Entamoeba species have mitosomes that are mitochondrion-derived organelles lacking DNA. Mitosomes isolated from E. histolytica were transplanted successfully into E. histolytica, E. nuttalli and E. invadens. Co-localization of mitosomal proteins from donor and recipient was observed in single mitosomes of some transplanted cells.