| Budget Amount *help |
¥167,310,000 (Direct Cost: ¥128,700,000、Indirect Cost: ¥38,610,000)
Fiscal Year 2015: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2014: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2013: ¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
Fiscal Year 2012: ¥35,750,000 (Direct Cost: ¥27,500,000、Indirect Cost: ¥8,250,000)
Fiscal Year 2011: ¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
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| Outline of Final Research Achievements |
The goal of this research project is to construct the synthetic genetic circuit which realizes a desired dynamic behavior. This circuit is composed of 10 or less biological molecular parts. In order to reach this goal, we accomplished 1) construction of genetic toggle switch operating with high stability, 2) application of genetic toggle switch, 3) modification of sensor proteins, 4) reconstruction and modification of quorum sensing system. We used not only molecular biology but also mathematical analysis for the system design of the synthetic genetic circuit. Through these researches, at last, we succeeded to construct “target product producing microorganism autonomously controlled” which could sense cell number and change intracellular metabolite distribution suitable for from cell growth to production according to cell number and produce 3 times more target product than the control strain.
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