| Budget Amount *help |
¥82,810,000 (Direct Cost: ¥63,700,000、Indirect Cost: ¥19,110,000)
Fiscal Year 2015: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2014: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2013: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2012: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2011: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
|
|---|
| Outline of Final Research Achievements |
To construct an in vitro model of hepatic tissue organogenesis by developing synthetic biological systems, we challenged to establish an adequate platform for synthetic approach in mammalian. Gene expression analysis was carried out at several liver developmental stages in mouse. Next, a hepatic endothelial cell strain was established as support cell for hepatic differentiation of mouse ES cells. To prepare in vitro system corresponding to early hepatic development period, conditional expression system of LIF and activin was constructed and was introduced into endothelial cell line to keep undifferentiation and endodermal differentiation of mouse ES cell, respectively. Mouse hepatic progenitor cells were cultured and differentiated to mature hepatocytes or cholagiocytes under the laminar flow-supplied different medium conditions in a same micro-fluidic device. In conclusion, we could show an adequate example as platform in consideration of cross talk for synthetic biology in mammal.
|
