| Budget Amount *help |
¥163,280,000 (Direct Cost: ¥125,600,000、Indirect Cost: ¥37,680,000)
Fiscal Year 2015: ¥28,990,000 (Direct Cost: ¥22,300,000、Indirect Cost: ¥6,690,000)
Fiscal Year 2014: ¥36,010,000 (Direct Cost: ¥27,700,000、Indirect Cost: ¥8,310,000)
Fiscal Year 2013: ¥33,410,000 (Direct Cost: ¥25,700,000、Indirect Cost: ¥7,710,000)
Fiscal Year 2012: ¥53,040,000 (Direct Cost: ¥40,800,000、Indirect Cost: ¥12,240,000)
Fiscal Year 2011: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
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| Outline of Final Research Achievements |
By evolutionary engineering method applied for natural RNP (RNA-protein complex), this research leads to preparation of novel RNP modules for control of signal transduction pathway. Also, a double RNA splicing reaction system was developed artificially, in which a pair of splicing ribozymes worked cooperatively to edit two distinct RNA sequences. This system can be employed as a module for RNA-based genetic circuits in vivo. As interdisciplinary studies with information science researchers, furthermore, we have demonstrated that the same results can be obtained as a result of the same perturbation, modulation of strength for promoters of artificial genetic circuits, both on living cell population and model established by information science and control engineering.
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