| Budget Amount *help |
¥83,460,000 (Direct Cost: ¥64,200,000、Indirect Cost: ¥19,260,000)
Fiscal Year 2015: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2014: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2013: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2012: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2011: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000)
|
|---|
| Outline of Final Research Achievements |
Conventional methods to construct synthetic gene regulatory networks (GRNs) need time-consuming trial and error steps, preventing their use in synthetic biology. Thus, we have developed a method to more rationally design and construct synthetic GRNs using the framework of a modular DNA computer RTRACS. Using the present method, we successfully built and drove a GRN in a cell-free protein synthesis system confined in a cell-sized vesicle. A GRN constructed in a vesicle by the present method can be basically integrated into a living cell using standard molecular cloning technology.
|
