| Budget Amount *help |
¥146,770,000 (Direct Cost: ¥112,900,000、Indirect Cost: ¥33,870,000)
Fiscal Year 2016: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2015: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2014: ¥28,210,000 (Direct Cost: ¥21,700,000、Indirect Cost: ¥6,510,000)
Fiscal Year 2013: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2012: ¥34,320,000 (Direct Cost: ¥26,400,000、Indirect Cost: ¥7,920,000)
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| Outline of Final Research Achievements |
In this research I aimed at understanding the molecular mechanism of cellular signal reception from the immune organs via direct cell-cell attachments using structural methods. The major methods include x-ray crystallography and electron microscopy (EM). As a result, we determined for the first time the three dimensional structure of the laminin receptor α6β1 integrin at atomic resolution. We also deduced the signaling mechanism of plexins, the semaphorin receptors, by using EM imaging technique. Other accomplishments include the isolation of a macrocyclic peptide that modulates plexinB1 signaling via allosteric mechanism, together with the clarification of its mode of action by crystallography. We also discovered multiple key molecules on neuronal cells or stem cells implicated in the homeostasis, and performed biochemical as well as structural analyses to elucidate inter-cellular signaling mechanisms in a broad biological contexts.
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