| Budget Amount *help |
¥208,260,000 (Direct Cost: ¥160,200,000、Indirect Cost: ¥48,060,000)
Fiscal Year 2016: ¥37,440,000 (Direct Cost: ¥28,800,000、Indirect Cost: ¥8,640,000)
Fiscal Year 2015: ¥37,440,000 (Direct Cost: ¥28,800,000、Indirect Cost: ¥8,640,000)
Fiscal Year 2014: ¥37,700,000 (Direct Cost: ¥29,000,000、Indirect Cost: ¥8,700,000)
Fiscal Year 2013: ¥37,440,000 (Direct Cost: ¥28,800,000、Indirect Cost: ¥8,640,000)
Fiscal Year 2012: ¥58,240,000 (Direct Cost: ¥44,800,000、Indirect Cost: ¥13,440,000)
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| Outline of Final Research Achievements |
Ciliogenesis is regulated by context-dependent cellular cues, including some transduced through appendage-like structures on ciliary basal bodies called transition fibers and basal feet. We examined the effects of Odf2 deletion constructs on ciliogenesis in Odf2-knockout F9 cells due to electron microscopy and revealed that during ciliogenesis, the different ODF2 fragments were required to form the distal and subdistal appendages (Da and Sa) in centrosomes which are related to transition fibers and basal feet (Tf and Bf), respectively. The Da and Tf were required for ciliogenesis. The Sa and Bf stabilized the microtubules associated. In the epithelial cell sheets, the apical cytoskeleton were formed differentially from centrosomal microtubules possibly with some relations unknown which stablize ciliogenesis and consisted of three layered structures of actin filaments, intermediate filaments, and microtubules, as revealed by the high voltage electron microscopic tomography.
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