| Budget Amount *help |
¥84,760,000 (Direct Cost: ¥65,200,000、Indirect Cost: ¥19,560,000)
Fiscal Year 2016: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2015: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2014: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2013: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2012: ¥28,600,000 (Direct Cost: ¥22,000,000、Indirect Cost: ¥6,600,000)
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| Outline of Final Research Achievements |
Microtubules, the main cytoskeletal components in eukaryotic cells, comprise globular αβ-tubulin dimers stacked head-to-tail to form protofilaments (pfs) that run lengthwise along the cell wall, thereby conferring structural polarity.Lis1 fixed the conformation of cytoplasmic dynein bound to a microtubule regardless of the nucleotide condition. This suggests that the NUDC (mNUDC) interacts with kinesin-1 and is required for the kinesin-1-idling state of cytoplasmic dynein on microtubules induced by Lis1 occurs through the Lis1-dependent arrest of dynein chemomechanical coupling. Mammalian mediated anterograde transport of a cytoplasmic dynein complex. Upon reaching the plus end of MTs, the small GTPase Rab6a releases LIS1 from the LIS1-dynein complex to allow the resumption of dynein motor activity. On the other hand, ADP-ribosylation factor-like 3 (Arl3) and the dynein light chain LC8 induce the dissociation of dynactin from cytoplasmic dynein to allow cargo unloading.
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