| Budget Amount *help |
¥111,670,000 (Direct Cost: ¥85,900,000、Indirect Cost: ¥25,770,000)
Fiscal Year 2016: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2015: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2014: ¥22,620,000 (Direct Cost: ¥17,400,000、Indirect Cost: ¥5,220,000)
Fiscal Year 2013: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2012: ¥21,710,000 (Direct Cost: ¥16,700,000、Indirect Cost: ¥5,010,000)
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| Outline of Final Research Achievements |
We performed a series of omics analyses on plant cell wall formation. Transcriptome/metabolome analysis revealed that the primary metabolism is strongly activated during secondary cell wall formation. Also, proteome analysis showed that the amount of several enzymes related to lignin biosynthesis and pectin degradation is drastically changed during secondary cell wall formation. On the analysis of primary cell wall formation, we established a system in which primary cell wall regeneration is induced from protoplasts of Arabidopsis cultured cells and mesophyll cells, using which we showed that KORRIGAN2 protein is closely associated with primary cell wall regeneration through transcriptome analysis and mutant analysis. Furthermore, we succeeded in indicating that in a moss plant, Physcomitrella patens, differentiation of water conducting cells (hydrioids) and supporting cells (stereids) is positively controlled by VNS genes, P. patens homologues of VND7.
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