Reorganization of cytoskeleton in response to extracellular stimuli

• Project Area: Plant cell wall as information-processing system
• Project/Area Number: 24114004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Hashimoto Takashi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (80180826)
• Co-Investigator(Renkei-kenkyūsha): KATO Takehide 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (70379535)
• Project Period (FY): 2012-06-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥73,840,000 (Direct Cost: ¥56,800,000、Indirect Cost: ¥17,040,000); Fiscal Year 2016: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2015: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2014: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2013: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2012: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 微小管 / 環境ストレス / チューブリン / リン酸化 / 植物 / 浸透圧ストレス / 乾燥ストレス / アラビドプシス / ストレス

Outline of Final Research Achievements

PHS1 was shown to phosphorylate Thr349 of alpha-tubulin, and the phosphorylated tubulin does not polymerize to microtubule polymer. In plant cells, the phosphatase domain suppresses the kinase domain of PHS1. In response to environmental stress (especially hyperosmotic stress), this suppression is cancelled and the kinase is activated. Liverwort and Chlamydomonas possess PHS1, which is activated by salt and hyperosmotic stresses. In vitro experiments with recombinant proteins show that PHS1 has basal kinase activity, which is directly inactivated by the MPK phosphatase activity within PHS1.

Research Products

• [Journal Article] Novel Arabidopsis microtubule-associated proteins track growing microtubule ends. (2017)
  • Author(s): J.H. Wong, and T. Hashimoto
  • Journal Title: BMC Plant Biol. Volume: 17 Issue: 1 Pages: 33-33
  • DOI: 10.1186/s12870-017-0987-5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Affinity purification and characterization of functional tubulin from cell suspension cultures of Arabidopsis and tobacco. (2016)
  • Author(s): T. Hotta, S. Fujita, S. Uchimura, M. Noguchi, T. Demura, E. Muto, and T. Hashimoto
  • Journal Title: Plant Physiol. Volume: 170 Pages: 1189-1205
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Roles of MAP65-1 and BPP1 in Gravity Resistance of Arabidopsis hypocotyls (2016)
  • Author(s): M. Murakami, K. Soga, T. Kotake, T. Kato, T. Hashimoto, K. Wakabayashi, and T. Hoson
  • Journal Title: Biological Sciences in Space Volume: 30 Issue: 0 Pages: 1-7
  • DOI: 10.2187/bss.30.1
  • NAID: 130005421496
  • ISSN: 0914-9201, 1349-967X
  • URL: https://ocu-omu.repo.nii.ac.jp/records/2019675
  • Peer Reviewed / Open Access
• [Journal Article] Abscisic acid induces ectopic outgrowth in epidermal cells through cortical microtubule reorganization in Arabidopsis thaliana. (2015)
  • Author(s): S. Takatani, T. Hirayama, T. Hashimoto, T. Takahashi, and H. Motose
  • Journal Title: Sci. Rep. Volume: 5 Pages: 11364-11364
  • NAID: 120005651882
  • Peer Reviewed
• [Journal Article] Microtubules in Plants (2015)
  • Author(s): T. Hashimoto
  • Journal Title: The Arabidopsis Book Volume: 13 Pages: e0179-e0179
  • DOI: 10.1199/tab.0179
  • Peer Reviewed
• [Journal Article] GCP-WD mediates g-TuRC recruitment and the geometry of microtubule nucleation in the acentrosomla interphase arrays of Arabidopsis. (2014)
  • Author(s): Walia et al.
  • Journal Title: Curr. Biol. Volume: 24 Issue: 21 Pages: 2548-2555
  • DOI: 10.1016/j.cub.2014.09.013
  • Peer Reviewed
• [Journal Article] α-tubulin is rapidly phosphorylated in response to hyperosmotic stress in rice and arabidopsis (2013)
  • Author(s): Yoshinori Ban, Yuhko Kobayashi, Tomomi Hara, Takahiro Hamada, Takashi Hashimoto, Shin Takeda, Tsukaho Hattori
  • Journal Title: Plant Cell Physiol. Volume: 54 Issue: 6 Pages: 848-858
  • DOI: 10.1093/pcp/pct065
  • Peer Reviewed
• [Journal Article] An atypical tubulin kinase mediates stress-induced microtubule depolymer ization in Arabidopsis (2013)
  • Author(s): Satoshi Fujita, Jaromir Pytela, Takashi Hotta, Takehide Kato, Takahiro Hamada, Rie Akamatsu, Yasumasa Ishida, Natsumaro Kutsuna, Seiichiro Hasezawa, Yuko Nomura, Hirofumi Nakagami, Takashi Hashimoto
  • Journal Title: Curr. Biol Volume: 23 Issue: 20 Pages: 1969-1978
  • DOI: 10.1016/j.cub.2013.08.006
  • Peer Reviewed
• [Journal Article] RNA Processing Bodies, Peroxisomes, Golgi Bodies, Mitochondria, and ER Tubule Junctions Frequently Pause at Cortical Microtubules (2012)
  • Author(s): Takahiro Hamada, Motoki Tominaga, Takeshi Fukaya, Masayoshi Nakamura, Akihiko Nakano, Yuichiro Watanabe, Takashi Hashimoto, Tobias I.Baskin
  • Journal Title: Plant and Cell Physiology Volume: 53 Issue: 4 Pages: 699-708
  • DOI: 10.1093/pcp/pcs025
  • Peer Reviewed
• [Journal Article] Arabidopsis GCP3-INTERACTING PROTEIN1/MOZAET1 is an integral component of the Y-tubulin-containing microtubule nucleating complex (2012)
  • Author(s): Nakamura M, Yagi N, Kato T, Fujita S, Kawashima N, Ehrhardt DW, Hashimoto T
  • Journal Title: The Plant Journal Volume: (In Press) Issue: 2 Pages: 216-225
  • DOI: 10.1111/j.1365-313x.2012.04988.x
  • Peer Reviewed
• [Presentation] 微小管結合タンパク質BPP ファミリーは葉表皮細胞の形態形成に関与する (2017)
  • Author(s): Wong Jeh Haur，加藤壮英，長崎(武内) 菜穂子，橋本隆
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] In-vitro Functional Analysis of Arabidopsis Tubulin Kinase PHS1 (2017)
  • Author(s): Duncan Coleman, Takashi Hotta, Takashi Hashimoto
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] PHS1 tubulin kinase is transiently activated by salt and hyperosmotic stresses in Arabidospis thaliana and Chlamydomonas reinhardtii (2017)
  • Author(s): Lee Mei Ng, Hideyuki Takahashi, Takashi Yamano, Hideya Fukuzawa, Takashi Hashimoto
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] Rapid and reversible tubulin phosphorylation regulates plant microtubule stability upon hyperosmotic stress (2016)
  • Author(s): Takashi Hashimoto
  • Organizer: EMBL Workshop: Microtubules
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2016-05-29
  • Int'l Joint Research
• [Presentation] チューブリンリン酸化酵素PHS1 はMAPK を介さずにリン酸化活性を抑制する (2016)
  • Author(s): Coleman Duncan，堀田 崇，橋本 隆
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 信州大学（長野県上田市）
  • Year and Date: 2016-03-18
• [Presentation] 器官のねじれ形質への各細胞層の寄与 (2016)
  • Author(s): 加藤 壮英，市村 朋子，平井 祐貴，足立 侑実子，寳金 佐和子，藤田 智史，八木 慎宜，橋本 隆
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 信州大学（長野県上田市）
  • Year and Date: 2016-03-18
• [Presentation] 精製植物チューブリンを用いた微小管のin vitroダイナミクス解析 (2016)
  • Author(s): 堀田 崇，内村 誠一，野口 真大，出村 拓，武藤 悦子，橋本 隆
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 信州大学（長野県上田市）
  • Year and Date: 2016-03-18
• [Presentation] 高浸透圧ストレスに応答したゼニゴケの表層微小管脱重合 (2016)
  • Author(s): 高橋 英之，長尾 直弥，西浜 竜一，河内 孝之，橋本 隆
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 信州大学（長野県上田市）
  • Year and Date: 2016-03-18
• [Presentation] ダイナミックな微小管細胞骨格は植物細胞の形を作り出し、環境ストレスに応答する (2015)
  • Author(s): 橋本隆
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学（石川県金沢市）
  • Year and Date: 2015-09-12
  • Invited
• [Presentation] Plant responses to drought by the cytoskeleton remodeling (2015)
  • Author(s): Takashi Hashimoto
  • Organizer: Annual Academic Meeting of Korean Society for Plant Biotechnology
  • Place of Presentation: Korean Cultural Center（Chungnam Province, Korea)
  • Year and Date: 2015-06-17
  • Int'l Joint Research / Invited
• [Presentation] TOG カラムを用いた植物培養細胞からのチューブリン精製 (2015)
  • Author(s): 堀田 崇，橋本 隆
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16
• [Presentation] ゼニゴケを用いたストレス誘導性の表層微小管脱重合機構の解析 (2015)
  • Author(s): 高橋 英之，長尾 直弥，西浜 竜一，河内 孝之，橋本 隆
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16
• [Presentation] TOGカラムを用いた植物材料からのチューブリン大量精製法とその応用 (2014)
  • Author(s): 堀田崇、藤田智史、橋本隆
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学、富山県富山市
• [Presentation] シロイヌナズナ新規微小管結合タンパク質ファミリーの機能解析 (2013)
  • Author(s): 長崎菜穂子
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 2013-03-21
• [Presentation] 微小管形成開始部位に局在するシロイヌナズナ新規MAPsの機能解析 (2013)
  • Author(s): 加藤壮英
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 2013-03-21
• [Presentation] Dual labeling of microtubules and nucleation complexes reveals detailed array assembly processes in Arabidopsis cells (2013)
  • Author(s): 八木慎宜
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 2013-03-21
• [Presentation] atypical kinase PHS1は高浸透圧条件下において微小管の脱重合を促進する (2013)
  • Author(s): 藤田智史
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 2013-03-21
• [Presentation] Organization of cortical microtubule arrays in Arabidopsis (2012)
  • Author(s): Takashi Hashimoto
  • Organizer: 10th International Congress of Plant Molecular Biology
  • Place of Presentation: Cheju, Korea
  • Year and Date: 2012-10-23
  • Invited
• [Remarks] 橋本研ホームページ
  • URL: http://bsw3.naist.jp/hashimoto/