Analyses of intracellular signaling in plant

• Project Area: Plant cell wall as information-processing system
• Project/Area Number: 24114009
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kumamoto University
• Principal Investigator: Sawa Shinichiro 熊本大学, 大学院先端科学研究部(理), 教授 (00315748)
• Co-Investigator(Kenkyū-buntansha): 相田 光宏 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (90311787)
• Project Period (FY): 2012-06-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥126,880,000 (Direct Cost: ¥97,600,000、Indirect Cost: ¥29,280,000); Fiscal Year 2016: ¥28,730,000 (Direct Cost: ¥22,100,000、Indirect Cost: ¥6,630,000); Fiscal Year 2015: ¥28,730,000 (Direct Cost: ¥22,100,000、Indirect Cost: ¥6,630,000); Fiscal Year 2014: ¥28,860,000 (Direct Cost: ¥22,200,000、Indirect Cost: ¥6,660,000); Fiscal Year 2013: ¥28,730,000 (Direct Cost: ¥22,100,000、Indirect Cost: ¥6,630,000); Fiscal Year 2012: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
• Keywords: シロイヌナズナ / 細胞外 / 細胞壁 / ペプチド / 植物感染性線虫 / 線虫感染 / 茎頂分裂組織 / CLE / CUC / サツマイモネコブセンチュウ / 細胞間相互作用 / 植物細胞壁 / 分裂組織 / センチュウ感染 / 生物間相互作用 / インテリジェント / 内生プログラム

Outline of Final Research Achievements

Plant cell wall is regarded as not only a dynamic structure responsible for the regulation of plant growth and differentiation, but as an intelligent system capable of processing information from the environment. To date, we do not understand the molecular mechanisms that render the cell wall capable of mediating information processing and self-regulation.
In this research, we examined importance of plant cell wall function in the aspect of plant parasitic nematode infection mechanisms and of regulation of plant meristem. We have identified nematode attractant. Further, we have also found many nematode effector protein and plant factors responsible for nematode infection to plants. On the other hand, we have identified downstream of CLE peptides as a signaling molecule which regulate plant meristem activity. Further, CUC signaling pathway was also examined.

Research Products

• [Int'l Joint Research] INRA(フランス)
• [Int'l Joint Research] UNIVERSIDAD DE CASTILLA LA MANCHA(スペイン)
• [Int'l Joint Research] Gent University(ベルギー)
• [Int'l Joint Research] Cambridge University(英国)
• [Int'l Joint Research] Oslo University(ノルウェー)
• [Journal Article] Chloroplastic ATP synthase builds up proton motive force for preventing reactive oxygen species production in photosystemI. (2017)
  • Author(s): Takagi, D., Amako, K., Hashiguchi, M., Fukaki, H., Ishizaki, K., Goh T., Fukao, Y., Sano, R., Kurata, T., Demura, T., Sawa, S., and Miyake C.
  • Journal Title: Plant J. Volume: In Press.
  • Peer Reviewed
• [Journal Article] Negative phototaxis in M. incognita (2017)
  • Author(s): Oota, M., Gotoh, E., Endo, M., Ishida, T., Matsushita, T., and Sawa, S.
  • Journal Title: Nem. Res. Volume: In Press.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Role of LOTR1 in nutrient transport through organization of spatial distribution of root endodermal barriers. (2017)
  • Author(s): Li, B., Kamiya, T., Kalmbach, L., Yamagami, M., Yamaguchi, K., Shigenobu S., Sawa, S., Danku, J., Salt, D., Geldner, N., and Fujiwara, T.
  • Journal Title: Curr. Biol. Volume: 27 Pages: 758-765
  • Peer Reviewed
• [Journal Article] CLE peptides and their signaling pathways in plant development. (2016)
  • Author(s): Yamaguchi, Y., Ishida, T.,Sawa, S.
  • Journal Title: J. Exp. Bot. Volume: 67 Issue: 16 Pages: 4813-4826
  • DOI: 10.1093/jxb/erw208
  • Peer Reviewed / Open Access
• [Journal Article] Polyamine resistance is increased by mutations in a nitrate transporter gene NRT1.3 (AtNPF6.4) in Arabidopsis thaliana. (2016)
  • Author(s): Tong W, Imai A, Tabata R, Shigenobu S, Yamaguchi K, Yamada M, Hasebe M, Sawa S, Motose H, Takahashi T
  • Journal Title: Front. Plant Sci. Volume: 7-834 Pages: 834-834
  • DOI: 10.3389/fpls.2016.00834
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Naming of Names: Guidelines for Gene Nomenclature in Marchantia (2016)
  • Author(s): Bowman, J., Araki, T., Arteaga-Vazquez, M., Berger, F., Dolan, L., Haseloff, J., Ishizaki, K., Kyozuka, J., Lin, S.S., Nagasaki, H., Nakagami, H., Nakajima, K., Nakamura, Y., Ohashi-Ito, K., Sawa, S., Shimamura, M., Solano, R., Tsukaya, H., Ueda, T., Watanabe, Y., Yamato, K.T., Zachgo, S., and Kohchi, T.
  • Journal Title: Plnat cell Physiology Volume: 57 Issue: 2 Pages: 257-261
  • DOI: 10.1093/pcp/pcv193
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Three-dimensional imaging of plant organs using a simple and rapid transparency technique. (2016)
  • Author(s): Hasegawa J, Sakamoto Y, Nakagami S, Aida M, Sawa S, Matsunaga S
  • Journal Title: Plant Cell Physiol Volume: 57 Issue: 3 Pages: 462-472
  • DOI: 10.1093/pcp/pcw027
  • NAID: 120005756556
  • Peer Reviewed / Open Access
• [Journal Article] Chemotaxis assay of plant-parasitic nematodes on a gel-filled microchannel device (2015)
  • Author(s): Hida, H., Nishiyama, H., Sawa, S., Higashiyama, T., Arata, H.
  • Journal Title: Sensors and Actuators Volume: B 221 Pages: 1483-1491
  • DOI: 10.1016/j.snb.2015.07.081
  • Peer Reviewed
• [Journal Article] BAM 1 and RECEPTOR-LIKE PROTEIN KINASE 2 constitute a signaling pathway and modulate CLE peptide-triggered growth inhibition in Arabidopsis root. (2015)
  • Author(s): Shimizu N, Ishida T, Yamada M, Shigenobu S, Tabata R, Kinoshita A, Yamaguchi K, Hasebe M, Mitsumasu K, Sawa S,
  • Journal Title: New phytologist Volume: 208 Issue: 4 Pages: 1104-1113
  • DOI: 10.1111/nph.13520
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 2.Plant peptide hormone signaling. (2015)
  • Author(s): Motomitsu A, Sawa S, Ishida T
  • Journal Title: Essays in Biochemistry Volume: 58 Pages: 115-131
  • DOI: 10.1042/bse0580115
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of parasitism of plant-parasitic nematodes at apoplast. (2015)
  • Author(s): Mitsumasu, K., Nishiyama, H., Nakagami, S., Kanemaru, Y. and Sawa, S
  • Journal Title: Jap. J. Pesticide Sci Volume: 40 Pages: 44-51
  • NAID: 130005096498
  • Peer Reviewed
• [Journal Article] Mystery in genetics: PUB4 gives a clue to the complex mechanism of CLV signaling pathway in the shoot apical meristem (2015)
  • Author(s): Kinoshita, A., Seo, M., Kamiya, Y., and Sawa, S.
  • Journal Title: Plant Signaling & Behavior Volume: 10:6 Issue: 6 Pages: e1028707-e1028707
  • DOI: 10.1080/15592324.2015.1028707
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Protocol for root-knot nematode culture by a hydroponic system and nematode inoculation to <i>Arabidopsi</i><i>s </i> (2015)
  • Author(s): Nishiyama, H., Ngan, B. T., Nagakami, S., Ejima, C., Ishida, T., and Sawa, S.
  • Journal Title: Nematological Research (Japanese Journal of Nematology) Volume: 45 Issue: 1 Pages: 45-49
  • DOI: 10.3725/jjn.45.45
  • NAID: 130005118607
  • ISSN: 0919-6765, 1882-3408
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A plant U-box protein, PUB4, regulates asymmetric cell division and cell proliferation in the root meristem. (2015)
  • Author(s): Kinoshita, A, Hove, C.A., Tabata, R., Yamada, M., Shimizu, N., Ishida, T., Yamaguchi K., Shigenobu, S., Takebayashi, Y., Iuchi, S., Kobayashi, M., Kurata, T., Wada, T., Seo, M., Hasebe, M., Bilou, I., Fukuda, H., Scheres, B., Heidstra, R., Sawa, S.
  • Journal Title: Development Volume: 142 Issue: 3 Pages: 444-453
  • DOI: 10.1242/dev.113167
  • Peer Reviewed / Open Access
• [Journal Article] Maturation processes and structures of secreted peptides in plants. (2014)
  • Author(s): Tabata, R. Sawa, S.
  • Journal Title: Front.Plant. Sci. Volume: 5 Pages: 1-6
  • DOI: 10.3389/fpls.2014.00311
  • Peer Reviewed
• [Journal Article] Light-dependent green gall formation induced by Meloidogyne incognita. (2014)
  • Author(s): Nishiyama, H., Nakagami, S., Todaka, A., Arita, T., Ishida, T., Sawa, S.
  • Journal Title: Nematology Volume: 16 Issue: 8 Pages: 889-893
  • DOI: 10.1163/15685411-00002814
  • Peer Reviewed
• [Journal Article] Heterotrimeric G proteins control stem cell proliferation through CLAVATA signaling in Arabidopsis. (2014)
  • Author(s): Ishida, T., Tabata, R., Yamada, M., Aida, M., Mitsumasu, K., Fujiwara, M., Yamaguchi, K., Shigenobu, S., Higuchi, M., Tsuji, H., Shimamoto, K., Hasebe, M., Fukuda, H., Sawa, S.
  • Journal Title: EMBO rep. Volume: 15 Issue: 11 Pages: 1202-1209
  • DOI: 10.15252/embr.201438660
  • Peer Reviewed
• [Journal Article] CLE19 peptide activity is regulated by SUPPRESSOR OF LLP1 1-mediated C-terminal processing in endosomes in Arabidopsis. (2013)
  • Author(s): Tamaki, T., Betsuyaku, S., Hamasaki, R., Fujiwara, M., Fukao, Y., Fukuda, H., and Sawa, S.
  • Journal Title: Plant J. Volume: 6 Pages: 970-981
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The function of CLE and other plant peptide hormones in root development (2013)
  • Author(s): Yamada, M., and Sawa, S.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 16 Pages: 1-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evolutionarily conserved CLE peptide signaling in plant development, symbiosis, and parasitism. (2013)
  • Author(s): Miyawaki, K.,et al.
  • Journal Title: Curr. Opin. Plant Biol. Volume: 16 Issue: 5 Pages: 598-606
  • DOI: 10.1016/j.pbi.2013.08.008
  • Peer Reviewed
• [Journal Article] CLAVATA3-like genes are differentially expressed in Grape Vine (Vitis vinifera) tissues (2013)
  • Author(s): R. Tominaga-Wada, Y. Nukumizu, T. Wada, T. Sawa, T. Tetsumura
  • Journal Title: J. Plant Physiol. Volume: 170 Issue: 15 Pages: 1379-1383
  • DOI: 10.1016/j.jplph.2013.04.013
  • Peer Reviewed
• [Journal Article] SUPPRESSOR OF LLP1 1-mediated C-terminal processing is critical for CLE19 peptide activity. (2013)
  • Author(s): Takayuki Tamaki
  • Journal Title: The Plant Journal Volume: 76 Issue: 6 Pages: 970-981
  • DOI: 10.1111/tpj.12349
  • Peer Reviewed
• [Presentation] 植物感染性線虫の誘引物質の探索 (2017)
  • Author(s): 澤進一郎
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2017-03-18
  • Invited
• [Presentation] 植物感染性線虫の感染過程における細胞壁成分の機能と役割 (2017)
  • Author(s): 大田守浩，Allen Yi-Lun Tsai，鈴木れいら，島岡知恵，石田喬志，澤進一郎
  • Organizer: 第58回植物生理学会年会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
  • Invited
• [Presentation] Molecular mechanisms of gall formation during nematode infection (2016)
  • Author(s): SAWA SHINICHIRO
  • Organizer: International Symposium 'Front Lines of Plant Cell Wall Research and Beyond'
  • Place of Presentation: 熱海
  • Year and Date: 2016-10-04
  • Int'l Joint Research / Invited
• [Presentation] EFC7 and EFC15 of Meloidogyne incognita are responsible for infection steps in Alabidopsis (2016)
  • Author(s): SAWA SHINICHIRO
  • Organizer: 32nd Symposium European Society of Nematologists
  • Place of Presentation: ポルト
  • Year and Date: 2016-08-27
  • Int'l Joint Research / Invited
• [Presentation] SOL1 and other peptidases are responsible for CLE peptide processing mechanisms (2016)
  • Author(s): SAWA SHINICHIRO
  • Organizer: 22nd International Conference on Plant Growth Substances
  • Place of Presentation: トロント大学
  • Year and Date: 2016-06-19
  • Int'l Joint Research / Invited
• [Presentation] Analysis of Arabidopsis attractant of plant parasitic nematode, M. incognita. (2016)
  • Author(s): Shinichiro Sawa
  • Organizer: 第57回日本植物生理学会
  • Place of Presentation: 岩手大学上田キャンパス
  • Year and Date: 2016-03-18
  • Int'l Joint Research / Invited
• [Presentation] “TOMEI”, a simple and quick clearing method for plant tissues (2015)
  • Author(s): Satoru Nakagami 3, Yuki Katsuyama 2, Shinichiro Sawa 3, Sachihiro Matsunaga
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium and Technical Workshop 2015
  • Place of Presentation: 名古屋大学野依記念学術交流館
  • Year and Date: 2015-08-27
  • Int'l Joint Research
• [Presentation] Analysis of plant attracta nt of plant parasitic nematode, M. incognita (2015)
  • Author(s): Shinichiro Sawa，Satoru Nakagami, Hidetaka Nishiyama, Yumi Kanemaru, Ayane Motomitsu
  • Organizer: Front Lines of Plant Cell Wall Research
  • Place of Presentation: 東大寺総合文化センター
  • Year and Date: 2015-03-20 – 2015-03-21
• [Presentation] Molecular dissection of CLE signaling pathway in Arabidopsis (2015)
  • Author(s): Shinichiro Sawa
  • Organizer: Cambridge University
  • Place of Presentation: Cambridge University
  • Year and Date: 2015-03-02
• [Presentation] Molecular dissection of the CLE signaling pathway on the infection steps of the M. incognita. (2014)
  • Author(s): Shinichrio Sawa, Ayane motomitsu, Hidetaka Nishiyama, Tomomi Sagara, Yumi Kanemaru, Satoru Nakagami, and Takashi Ishida
  • Organizer: 日韓合同線虫学シンポジウム
  • Place of Presentation: 文部科学省研究交流センター
  • Year and Date: 2014-09-18 – 2014-09-19
• [Presentation] Comprehensive genetic analysis of CLV3 downstream pathway in Arabidopsis. (2014)
  • Author(s): Shinichiro Sawa
  • Organizer: 第４７回日本発生生物学会
  • Place of Presentation: WINC AICHI
  • Year and Date: 2014-05-27 – 2014-05-30
• [Presentation] 植物感染性線虫の感染分子機構の解析 (2014)
  • Author(s): 澤進一郎、光増可奈子
  • Organizer: 日本農薬学会
  • Place of Presentation: 京都大学
• [Presentation] Comprehensive genetic analysis of CLV3 downstream pathway in Arabidopsis (2013)
  • Author(s): Shinichiro Sawa
  • Organizer: INTERNATIONAL KICK-OFF SYMPOSIUM FOR ISMCBC
  • Place of Presentation: Kusunoki Reception Room, Kumamoto University
  • Year and Date: 2013-12-02
• [Presentation] Participation of the cell wall interface in intercellular signaling in plants (2013)
  • Author(s): Shinichiro Sawa
  • Organizer: nternational Cell Wall Integrity Symposium
  • Place of Presentation: KKR Kamakura Wakamiya
  • Year and Date: 2013-10-31
• [Presentation] Carboxypeptidase in CLE peptide maturation step,and CLE signaling molecules in Arabidopsis (2013)
  • Author(s): Shinichiro Sawa
  • Organizer: 21ST Conference of the International Plant Growth Substances Association
  • Place of Presentation: The Shanghai International Conference Center(China)
• [Presentation] シロイヌナズナの幹細胞活性を規定するCLV3ペプチドホルモンシグナル伝達因子の順遺伝学による網羅的探索 (2013)
  • Author(s): 澤進一郎,志水法子,江島千佳,西山英孝,石田喬志
  • Organizer: 日本生化学大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Participation of the cell wall interface in intercellular signaling in plants (2013)
  • Author(s): Shinichiro Sawa
  • Organizer: International Cell Wall Integrity Symposium
  • Place of Presentation: KKR Kamakura Wakamiya
• [Book] 植物ゲノム解析、次世代シーケンサー活用術 (2016)
  • Author(s): 重信秀治、澤進一郎、栗原志、持田恵一、上原（山口）由紀子、高木宏樹、阿部陽、寺内良平、関原明、高橋聡史
  • Publisher: 化学同人
• [Book] 細胞外空間（細胞壁）に注目した植物寄生性線虫の感染分子機構 (2016)
  • Author(s): 光増可奈子，西山英孝，中上知，金丸由実，澤進一郎
  • Publisher: 日本農薬学会誌
• [Book] Analysis of attractant of plant parasitic nematode (2016)
  • Author(s): Sawa, S
  • Publisher: Soy Protein Research
• [Remarks] 澤研究室ホームページ
  • URL: http://www.sci.kumamoto-u.ac.jp/~sawa/