Studies on the movement and functions of plant hormones in the space outside the cell

• Project Area: Plant cell wall as information-processing system
• Project/Area Number: 24114010
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tohoku University
• Principal Investigator: Yamaguchi Shinjiro 東北大学, 生命科学研究科, 教授 (10332298)
• Research Collaborator: MASHIGUCHI Kiyoshi 東北大学, 生命科学研究科, 助教 (00569725)
• Project Period (FY): 2012-06-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥59,150,000 (Direct Cost: ¥45,500,000、Indirect Cost: ¥13,650,000); Fiscal Year 2016: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000); Fiscal Year 2015: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000); Fiscal Year 2014: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000); Fiscal Year 2013: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000); Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 植物ホルモン / 植物細胞壁 / ストリゴラクトン / 植物細胞外空間 / 輸送体 / アポプラスト / 植物 / 生理活性 / 生体分子 / 発生・分化

Outline of Final Research Achievements

In this study, we aimed at elucidating the roles of the space outside of the cell as a route for the movement of low-molecular-weight signaling molecules. In particular, we focused on strigolactones, which act as plant hormones and also as allelochemicals. We found that carotenoid-derived carlactone is an intermediate of strigolactone biosynthesis, and that carlactone is then converted to carlactonoic acid in Arabidopsis. We also found that a hydroxylated carlactonoic acid was detected in the xylem sap in various plant species. These results suggested that the hydroxylated carlactonoic acid might be a mobile form in the strigolactone biosynthetic pathway.

Research Products

• [Journal Article] LATERAL BRANCHING OXIDOREDUCTASE acts in the final stages of strigolactone biosynthesis in Arabidopsis (2016)
  • Author(s): Brewer, P.B., Yoneyama, K., Filardo, F., Meyers, E., Scaffidi, A., Frickey, T., Akiyama, K., Seto, Y., Dun, E.A., Cremer, J.E., Kerr, S.C., Waters, M.T., Flematti, G.R., Mason, M.G., Weiller, G., Yamaguchi, S., Nomura, T., Smith, S.M., Yoneyama, K., Beveridge, C.A.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 113 Issue: 22 Pages: 6301-6306
  • DOI: 10.1073/pnas.1601729113
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural requirements of strigolactones for shoot branching inhibition in rice and Arabidopsis (2015)
  • Author(s): Mikihisa Umehara, Mengmeng Cao, Kohki Akiyama, Tomoki Akatsu, Yoshiya Seto, Atsushi Hanada, Li Weiqiang, Noriko Takeda-Kamiya, Yu Morimoto, Shinjiro Yamaguchi
  • Journal Title: Plant and cell physiology Volume: 印刷中 Issue: 6 Pages: 1059-1072
  • DOI: 10.1093/pcp/pcv028
  • Peer Reviewed
• [Journal Article] Carlactone is converted to carlactonoic acid by MAX1 in Arabidopsis and its methyl ester can directly interact with AtD14 in vitro (2014)
  • Author(s): Abe, S., Sado, A., Tanaka, K., Kisugi, T., Asami, K., Ota, S., Kim, H.I., Yoneyama, K., Xie, X., Ohnishi, T., Seto, Y., Yamaguchi, S., Akiyama, K., Yoneyama, K., Nomura, T.
  • Journal Title: Proc Natl Acad Sci USA Volume: 111 Issue: 50 Pages: 18084-18089
  • DOI: 10.1073/pnas.1410801111
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Strigolactone biosynthesis and perception (2014)
  • Author(s): Seto, Y., Yamaguchi, S.
  • Journal Title: Curr Opin Plant Biol Volume: 21 Pages: 1-6
  • DOI: 10.1016/j.pbi.2014.06.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Carlactone is an endogenous biosynthetic precursor for strigolactones (2014)
  • Author(s): 瀬戸義哉
  • Journal Title: Proc Natl Acad Sci U S A Volume: 111 Issue: 4 Pages: 1640-1645
  • DOI: 10.1073/pnas.1314805111
  • Peer Reviewed
• [Journal Article] Recent advances in strigolactone research: chemical and biological aspects. (2012)
  • Author(s): Yoshiya Seto
  • Journal Title: Plant Cell Physiology Volume: 53 Issue: 11 Pages: 1843-1853
  • DOI: 10.1093/pcp/pcs142
  • Peer Reviewed
• [Journal Article] The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis. (2012)
  • Author(s): Yoshida, S., Kameoka, H., Tempo, M., Akiyama, K., Umehara, M., Yamaguchi, S., Hayashi, H., Kyozuka, J., Shirasu, K.
  • Journal Title: New Phytologist Volume: 196 Issue: 4 Pages: 1208-1216
  • DOI: 10.1111/j.1469-8137.2012.04339.x
  • Peer Reviewed
• [Presentation] Strigolactone biosynthesis and its regulation (2017)
  • Author(s): Shinjiro Yamaguchi
  • Organizer: 2nd International Congress on Strigolactones
  • Place of Presentation: Turin (Italy)
  • Year and Date: 2017-03-27
  • Int'l Joint Research / Invited
• [Presentation] Strigolactone biosynthesis and action in rice and Arabidopsis (2016)
  • Author(s): Shinjiro Yamaguchi
  • Organizer: 22nd International Conference on Plant Growth Substances
  • Place of Presentation: Toronto (Canada)
  • Year and Date: 2016-06-21
  • Int'l Joint Research / Invited
• [Presentation] Identification of carlactonoic acid methyltransferase in Arabidopsis (2016)
  • Author(s): Yuta Onozuka, Naoki Kitaoka, Takaya Kisugi, Kohki Akiyama, Kiyoshi Mashiguchi, Yoshiya Seto, Shinjiro Yamaguchi
  • Organizer: 22nd International Conference on Plant Growth Substances
  • Place of Presentation: Toronto (Canada)
  • Year and Date: 2016-06-21
  • Int'l Joint Research
• [Presentation] カーラクトン酸メチル化酵素の同定 (2015)
  • Author(s): 小野塚祐太、北岡直樹、来生貴也、秋山康紀、瀬戸義哉、山口信次郎
  • Organizer: 植物化学調節学会第50回大会
  • Place of Presentation: 東京大学（東京都・文京区）
  • Year and Date: 2015-10-23
• [Presentation] カーラクトン酸メチルはシロイヌナズナの老化葉に蓄積する (2015)
  • Author(s): 東大野むつみ、森本優、瀬戸義哉、秋山康紀、山口信次郎
  • Organizer: 植物化学調節学会第50回大会
  • Place of Presentation: 東京大学（東京都・文京区）
  • Year and Date: 2015-10-23
• [Presentation] ストリゴラクトンの生合成及び分泌阻害剤の探索 (2015)
  • Author(s): 櫻田茜、田中海、笠原博幸、伊藤晋作、瀬戸義哉、山口信次郎
  • Organizer: 日本ケミカルバイオロジー学会 第10回年会
  • Place of Presentation: 東北大学（宮城県・仙台）
  • Year and Date: 2015-06-10
• [Presentation] 枝分かれ抑制ホルモンの生合成におけるメチル化酵素の探索 (2015)
  • Author(s): 小野塚祐太、北岡直樹、秋山康紀、瀬戸義哉、山口信次郎
  • Organizer: 日本ケミカルバイオロジー学会 第10回年会
  • Place of Presentation: 東北大学（宮城県・仙台）
  • Year and Date: 2015-06-10
• [Presentation] Identification of endogenous biosynthetic precursors for strigolactones in rice and Arabidopsis (2015)
  • Author(s): Yamaguchi, S.
  • Organizer: 1st International Congress on Strigolactones
  • Place of Presentation: Wageningen (The Netherlands)
  • Year and Date: 2015-03-01 – 2015-03-06
  • Invited
• [Presentation] ストリゴラクトン生合成中間体 carlactonoic acid の同定 (2014)
  • Author(s): 田中海、佐渡愛香、浅見慶、瀬戸義哉、秋山康紀、山口信次郎
  • Organizer: 植物化学調節学会第49回大会
  • Place of Presentation: 京都大学（京都府・京都）
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] シロイヌナズナにおけるカーラクトンの動態 (2014)
  • Author(s): 浅見慶、佐渡愛香、来生貴也、花田篤志、瀬戸義哉、秋山康起、山口信次郎
  • Organizer: 植物化学調節学会第49回大会
  • Place of Presentation: 京都大学（京都府・京都）
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] Strigolactone biosynthesis and action (2014)
  • Author(s): Yamaguchi, S.
  • Organizer: The 38th Naito Conference on Molecule-based biological systems
  • Place of Presentation: Chateraise Gateaux Kingdom (Hokkaido, Sapporo)
  • Year and Date: 2014-10-07 – 2014-10-10
  • Invited
• [Presentation] ストリゴラクトンの動態と機能 (2014)
  • Author(s): 山口信次郎
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] 安定同位体標識カーラクトンを用いたシロイヌナズナにおけるストリゴラクトンの生合成研究 (2014)
  • Author(s): 瀬戸義哉
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] ストリゴラクトン生合成におけるカーラクトンの関与 (2013)
  • Author(s): 浅見慶
  • Organizer: 植物化学調節学会 第48回大会
  • Place of Presentation: 新潟大学
• [Presentation] ストリゴラクトン動態と機能 (2012)
  • Author(s): 山口信次郎
  • Organizer: 日本植物学会 第76回大会
  • Place of Presentation: 兵庫県立大学
  • Year and Date: 2012-09-15
• [Remarks] 活性分子動態分野ホームページ
  • URL: http://www.lifesci.tohoku.ac.jp/teacher/t_yamaguchi/
• [Remarks] 活性分子動態分野ホームページ
  • URL: http://labo.lifesci.tohoku.ac.jp/biomol/
• [Remarks] 活性分子動態分野ホームページ
  • URL: http://www.lifesci.tohoku.ac.jp/research/bioactivemolecules/