Host cell competnecy for negative-stranded RNA viruses

• Project Area: Molecular basis of host cell competency in virus infection
• Project/Area Number: 24115002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: University of Tsukuba
• Principal Investigator: NAGATA Kyosuke 筑波大学, 学長 (40180492)
• Co-Investigator(Kenkyū-buntansha): 朴 三用 横浜市立大学, 生命医科学研究科, 教授 (20291932)
• Research Collaborator: Kawaguchi Atsushi 筑波大学, 医学医療系, 助教 (90532060)
• Project Period (FY): 2012-06-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥143,130,000 (Direct Cost: ¥110,100,000、Indirect Cost: ¥33,030,000); Fiscal Year 2016: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000); Fiscal Year 2015: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000); Fiscal Year 2014: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000); Fiscal Year 2013: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000); Fiscal Year 2012: ¥34,320,000 (Direct Cost: ¥26,400,000、Indirect Cost: ¥7,920,000)
• Keywords: インフルエンザウイルス / RNAポリメラーゼ / RNP / エンドソーム / 微小管 / 小胞輸送 / ウイルス / 宿主因子 / ゲノム複製 / 転写 / RNA依存性RNAポリメラーゼ / 構造生物学 / 感染症 / 分子生物学

Outline of Final Research Achievements

This research aimed to identify host factors, which regulate influenza virus genome function, and to analyze the replicational and post-replicational regulation of influenza virus genome.
We identified pp32/APRIL and Prp18 as stimulatory factors of viral RNA polymerase. Further, we found that newly synthesized viral genomes are recruited to the centrosome by YB-1, and the progeny viral genome is transported to the plasma membrane via cholesterol-enriched Rab11a-positive recycling endosomes. Cholesterol is thought to be a trigger for the virus assembly concomitantly with arrival of the virus genome beneath the plasma membrane.

Research Products

• [Int'l Joint Research] ボルドー大学(フランス)
• [Int'l Joint Research] ボルドー大学(フランス)
• [Journal Article] Mitotic phosphorylation of CCCTC-binding factor (CTCF) reduces its DNA binding activity. (2017)
  • Author(s): Sekiya T, Murano K, Kato K, Kawaguchi A, Nagata K.
  • Journal Title: FEBS Open Bio Volume: 7 Pages: 397-404
  • DOI: 10.1002/2211-5463.12189
  • NAID: 120006224664
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pre-mRNA processing factor Prp18 is a stimulatory factor of influenza virus RNA synthesis and possesses nucleoprotein chaperone activity. (2017)
  • Author(s): Minakuchi M, Sugiyama K, Kato Y, Naito T, Okuwaki M, Kawaguchi A, Nagata K.
  • Journal Title: J. Virol. Volume: 91
  • DOI: 10.1128/jvi.01398-16
  • NAID: 120006023423
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Efficient DNA binding of NF-κB requires the chaperone-like function of NPM1 (2017)
  • Author(s): Lin J, Kato M, Nagata K, Okuwaki M.
  • Journal Title: Nucleic Acids Res Volume: ｇｋｗ1285 Pages: 3703-3723
  • DOI: 10.1093/nar/gkw1285
  • NAID: 120006223991
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Generation of a Genetically Stable High-Fidelity Influenza Vaccine Strain. (2017)
  • Author(s): Naito T, Mori K, Ushirogawa H, Takizawa N, Nobusawa E, Odagiri T, Tashiro M, Ohniwa RL, Nagata K, Saito M.
  • Journal Title: Journal of Virology Volume: 91
  • Peer Reviewed
• [Journal Article] Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1. (2016)
  • Author(s): Asaka MN, Kawaguchi A, Sakai Y, Mori K, Nagata K.
  • Journal Title: Scientific Reports Volume: 6 Pages: 33608-33608
  • DOI: 10.1038/srep33608
  • NAID: 120005851332
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Functional characterization and efficient detection of Nucleophosmin/NPM1 oligomers. (2016)
  • Author(s): Lin J, Hisaoka M, Nagata K, Okuwaki M.
  • Journal Title: Biochemical Biophysical Research Communications Volume: 480 Pages: 702-708
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The Role of Nuclear Antiviral Factors against Invading DNA Viruses: The Immediate Fate of Incoming Viral Genomes. (2016)
  • Author(s): Komatsu T, Nagata K, Wodrich H.
  • Journal Title: Viruses Volume: 8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Tracking adenovirus genomes identifies morphologically distinct late DNA replication compartments. (2016)
  • Author(s): Komatsu T, Robinson DR, Hisaoka M, Ueshima S, Okuwaki M, Nagata K, Wodrich H.
  • Journal Title: Traffic Volume: 17 Pages: 1168-1180
  • DOI: 10.1111/tra.12429
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Regulation of Cellular Dynamics and Chromosomal Binding Site Preference of Linker Histones H1.0 and H1.X. (2016)
  • Author(s): Okuwaki M, Abe M, Hisaoka M, Nagata K.
  • Journal Title: Molecular and Cellular Biology Volume: 36 Pages: 2681-2696
  • Peer Reviewed
• [Journal Article] Leukemia-Associated Nup214 Fusion Proteins Disturb the XPO1-Mediated Nuclear-Cytoplasmic Transport Pathway and Thereby the NF-κB Signaling Pathway. (2016)
  • Author(s): Saito S, Cigdem S, Okuwaki M, Nagata K.
  • Journal Title: Mol. Cell Biol. Volume: 36 Pages: 1820-1835
  • DOI: 10.1128/mcb.00158-16
  • NAID: 120005825940
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion. (2016)
  • Author(s): Moriyama, M. et al.
  • Journal Title: Journal of Virology Volume: 90 Pages: 4105-14
  • DOI: 10.1128/jvi.00120-16
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Influenza virus induces cholesterol-enriched endocytic recycling compartments for budozone formation via cell cycle-independent centrosome maturation (2015)
  • Author(s): Kawaguchi A, Hirohama M, Harada Y, Osari S, Nagata K.
  • Journal Title: PLoS pathogens Volume: 11
  • DOI: 10.1371/journal.ppat.1005284
  • NAID: 120005713086
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNA (2015)
  • Author(s): Sugiyama K, Kawaguchi A, Okuwaki M, Nagata K.
  • Journal Title: eLife Volume: 4
  • DOI: 10.7554/elife.08939
  • NAID: 120005764042
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Measles virus C protein facilitates transcription by the control of N protein-viral genomic RNA interaction in early phases of infection (2015)
  • Author(s): Nishie T, Nagata K.
  • Journal Title: BIochem Biophys Res Commune Volume: 463 Pages: 1262-1266
  • DOI: 10.1016/j.bbrc.2015.06.099
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A method for visualization of incoming adenovirus chromatin complexes in fixed and living cell (2015)
  • Author(s): Komatsu T, Dacheux D, Kreppel F, Nagata K, Wodrich H.
  • Journal Title: PLoS One Volume: 10
  • DOI: 10.1371/journal.pone.0137102
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] An adenovirus DNA replication factor, but not incoming genome complexes, targets PML nuclear bodies (2015)
  • Author(s): Komatsu T, Nagata K, Wodrich H.
  • Journal Title: J. Virol. Volume: 90 Pages: 1657-1667
  • DOI: 10.1128/jvi.02545-15
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Tamiflu Expands Quasispecies of Influenza Virus through Cell-to-cell Transmission. (2015)
  • Author(s): Mori K, Murano K, Ohniwa RL, Kawaguchi A, Nagata K.
  • Journal Title: Scientific Report Volume: 5 Pages: 9163-9163
  • DOI: 10.1038/srep09163
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Actin-myosin network is required for proper assembly of influenza virus particles. (2015)
  • Author(s): Kumakura M, Kawaguchi A, Nagata K.
  • Journal Title: Virology Volume: 476 Pages: 141-150
  • DOI: 10.1016/j.virol.2014.12.016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Centrosome maturation requires YB-1 to regulate dynamic instability of microtubules for nucleus reassembly. (2015)
  • Author(s): Kawaguchi A, Asaka MN, Matsumoto K, Nagata K.
  • Journal Title: Scientific Reports Volume: 5 Pages: 8768-8768
  • DOI: 10.1038/srep08768
  • NAID: 120005596190
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Glucocorticoids facilitate the transcription from the human cytomegalovirus major immediate early promoter in glucocorticoid receptor- and nuclear factor-I-like protein-dependent manner (2015)
  • Author(s): Inoue-Toyoda M, Kato K, Nagata K, Yoshikawa H.
  • Journal Title: Biochem Biophys Res Commun Volume: 476 Pages: 9-14
  • DOI: 10.1016/j.bbrc.2015.01.091
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli (2014)
  • Author(s): Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K and Akimitsu N.
  • Journal Title: Mol. Cell Volume: 53 Pages: 393-406
  • DOI: 10.1016/j.molcel.2014.01.009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Involvement of the N-terminal portion of influenza virus RNA polymerase subunit PB1 in nucleotide recognition (2014)
  • Author(s): Binh NT, Wakai C, Kawaguchi A, Nagata K.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 443 Pages: 975-979
  • DOI: 10.1016/j.bbrc.2013.12.071
  • NAID: 120005399277
  • Peer Reviewed
• [Journal Article] Upstream binding factor-dependent and pre-rRNA transcription-independent association of pre-rRNA processing factors with rRNA gene (2014)
  • Author(s): Ueshima, S., Nagata, K., and Okuwaki, M.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 443 Pages: 22-27
  • DOI: 10.1016/j.bbrc.2013.11.039
  • Peer Reviewed
• [Journal Article] Intrinsically disordered regions of nucleophosmin/B23 regulate its RNA binding activity through their inter- and intra-molecular association (2014)
  • Author(s): Hisaoka, M., Nagata, K., and Okuwaki, M.
  • Journal Title: Nucleic Acids Research Volume: 42 Pages: 1180-1195
  • DOI: 10.1093/nar/gkt897
  • NAID: 120005399276
  • Peer Reviewed
• [Journal Article] The N-terminal region of influenza virus polymerase PB1 adjacent to the PA binding site is involved in replication but not transcription of the viral genome. (2013)
  • Author(s): Binh NT, Wakai C, Kawaguchi A, Nagata K.
  • Journal Title: Frontiers in Microbiology Volume: 4 Pages: 398-398
  • NAID: 120005399256
  • Peer Reviewed
• [Journal Article] DNA replication-dependent binding of CTCF plays a critical role in adenovirus genome functions (2013)
  • Author(s): Komatsu T, Sekiya T, Nagata K.
  • Journal Title: Scientific Reports Volume: 3 Pages: 2187-2187
  • DOI: 10.1038/srep02187
  • NAID: 120005316259
  • Peer Reviewed
• [Journal Article] Anti-influenza virus activity of Ginkgo biloba leaf extracts (2013)
  • Author(s): Haruyama T, Nagata K.
  • Journal Title: J. Nat. Med Volume: 67(3) Pages: 636-642
  • DOI: 10.1007/s11418-012-0725-0
  • Peer Reviewed
• [Journal Article] Identification of a Novel and Unique Transcription Factor in the Intraerythrocytic Stage of Plasmodium falciparum (2013)
  • Author(s): Komaki-Yasuda, K., Okuwaki, M., Nagata, K., Kawazu, S., and Kano, S.
  • Journal Title: PLOS ONE Volume: 8
  • DOI: 10.1371/journal.pone.0074701
  • Peer Reviewed
• [Journal Article] Antagonistic activities of the immunomodulator and PP2A-activating drug FTY720 (Fingolimod, Gilenya) in Jak2-driven hematologic malignancies (2013)
  • Author(s): Oaks JJ, Santhanam R, Walker CJ, Roof S, Harb JG, Ferenchak G, Eisfeld AK, Van Brocklyn JR, Briesewitz R, Saddoughi SA, Nagata K, Bittman R, Caligiuri MA, Abdel-Wahab O, Levine R, Arlinghaus RB, Quintas-Cardama A, Goldman JM, Apperley J, Reid A, Milojkovic D, Ziolo MT, Marcucci G, Ogretmen B, Neviani P, Perrotti D.
  • Journal Title: Blood Volume: 122(11) Pages: 1923-1934
  • DOI: 10.1182/blood-2013-03-492181
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic analysis of an Escherichia coli-selected mutant of the nuclease domain of the metallonuclease colicin E7 (2013)
  • Author(s): 10) Czene A, Tóth E, Gyurcsik B, Otten H, Poulsen JC, Lo Leggio L, Larsen S, Christensen HE, Nagata K.
  • Journal Title: Acta Crystallogr. Sect. F. Struct. Biol. Cryst. Commun Volume: 69 Pages: 551-554
  • DOI: 10.1107/s1744309113008233
  • Peer Reviewed
• [Journal Article] The role of the N-terminal loop in the function of the colicin E7 nuclease domain. (2013)
  • Author(s): Czene A, Németh E, Zóka IG, Jakab-Simon NI, Körtvélyesi T, Nagata K, Christensen HE, Gyurcsik B
  • Journal Title: Journal of Biological Inorganic Chemistry Volume: 18 Pages: 309-321
  • DOI: 10.1007/s00775-013-0975-7
  • Peer Reviewed
• [Journal Article] YB-1 functions as a porter to lead influenza virus ribonucleoprotein complexes to microtubules. (2012)
  • Author(s): Kawaguchi, A., Matsumoto, K., and Nagata, K.
  • Journal Title: Journal of Virology Volume: 86 Pages: 11086-11095
  • DOI: 10.1128/jvi.00453-12
  • NAID: 120005399279
  • Peer Reviewed
• [Journal Article] Replication-uncoupled histone deposition during adenovirus DNA replication. (2012)
  • Author(s): Komatsu T.
  • Journal Title: Journal of Virology Volume: 86(12) Pages: 6701-6711
  • DOI: 10.1128/jvi.00380-12
  • Peer Reviewed
• [Journal Article] Critical Role of an Antiviral Stress Granule Containing RIG-I and PKR in Viral Detection and Innate Immunity. (2012)
  • Author(s): Onomoto K, Jogi M, Yoo J-S, Narita R, Morimoto S, Takemura A, Sambhara S, Kawaguchi A, Osari S, Nagata K, Matsumiya T, Namiki H, Yoneyama M, Fujita T
  • Journal Title: PLoS ONE Volume: 7
  • DOI: 10.1371/journal.pone.0043031
  • Peer Reviewed
• [Journal Article] B23/Nucleophosmin is involved in regulation of adenovirus chromatin structure at late infection stages, but not in its replication and transcription (2012)
  • Author(s): Abdus Samad
  • Journal Title: J Gen Virol Volume: 93 Pages: 1328-1338
  • DOI: 10.1099/vir.0.036665-0
  • Peer Reviewed
• [Journal Article] Cell tropism and pathogenicity of measles virus in monkeys. (2012)
  • Author(s): Kato SI., et al.
  • Journal Title: Frontiers in Microbiology Volume: (in press) Pages: 14-14
  • DOI: 10.3389/fmicb.2012.00014
  • Peer Reviewed
• [Journal Article] Function of homo- and hetero-oligomers of human nucleoplasmin/nucleophosmin family proteins NPM1, NPM2, and NPM3 during sperm chromatin remodeling. (2012)
  • Author(s): Okuwaki M, Sumi A, Hisaoka M, Saotome-Nakamura A, Akashi S, Nishimura Y, Nagata K
  • Journal Title: Nucleic Acids Res. Volume: 40 Pages: 17607-17617
  • DOI: 10.1093/nar/gks162
  • Peer Reviewed
• [Journal Article] New sandwich-type enzyme-linked immunosorbent assay for human MxA protein in a whole blood using monoclonal antibodies against GTP-binding domain for recognition of viral infection. (2012)
  • Author(s): Kawamura M.
  • Journal Title: Journal of Clinical Laboratory Analysis Volume: 26 Pages: 174-183
  • DOI: 10.1002/jcla.21507
  • Peer Reviewed
• [Presentation] Viral budozone formation in concert with influenza virus genome transport using recycling endosomes. (2016)
  • Author(s): Kuroki T, Nagata K, Kawaguchi A.
  • Organizer: The 64th Annual Meeting of the Japanese Society for Virology
  • Place of Presentation: 札幌コンベンションセンター・北海道・札幌
  • Year and Date: 2016-10-23
• [Presentation] In situ detection of M1-NS2 complex formation essential for nuclear export of the influenza virus genome. (2016)
  • Author(s): Yamashita S, Asaka MN, Nagata K, Kawaguchi A.
  • Organizer: The 64th Annual Meeting of the Japanese Society for Virology
  • Place of Presentation: 札幌コンベンションセンター・北海道・札幌
  • Year and Date: 2016-10-23
• [Presentation] Influenza A virus infection induces Caspase-1-dependent cell death with pro-inflammatory cytokines in human lung epithelial cells. (2016)
  • Author(s): Lee SJ, Nagata K, Kawaguchi A.
  • Organizer: The 64th Annual Meeting of the Japanese Society for Virology
  • Place of Presentation: 札幌コンベンションセンター・北海道・札幌
  • Year and Date: 2016-10-23
• [Presentation] Intracellular trafficking of influenza virus genome via centrosome maturation. (2015)
  • Author(s): Kawaguchi A.
  • Organizer: The 38th Annual meeting of the Molecular Biology Society for Japan
  • Place of Presentation: 神戸国際会議場（神戸）
  • Year and Date: 2015-12-04
  • Int'l Joint Research / Invited
• [Presentation] Influenza virus induces cholesterol-enriched endocytic recycling compartments for budozone formation through centrosome maturation. (2015)
  • Author(s): Kawaguchi A, Hirohama M, Nagata K.
  • Organizer: The 63rd Annual Meeting of the Japanese Society for Virology
  • Place of Presentation: 福岡国際会議場（福岡）
  • Year and Date: 2015-11-23
  • Int'l Joint Research
• [Presentation] RNA replicon as proper noun (2015)
  • Author(s): Nagata K.
  • Organizer: The 63rd Annual Meeting of the Japanese Society for Virology
  • Place of Presentation: 福岡国際会議場（福岡）
  • Year and Date: 2015-11-22
  • Int'l Joint Research
• [Presentation] Novel host factor-dependent influenza A virus vRNA synthesis from its cRNA. (2015)
  • Author(s): Sugiyama K, Kawaguchi A, Okuwaki M, Nagata K.
  • Organizer: The 14th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場（淡路）
  • Year and Date: 2015-09-09
  • Int'l Joint Research
• [Presentation] Intracellular trafficking of influenza virus genome mediated by endocytic recycling compartments located at the centrosome. (2015)
  • Author(s): Kawaguchi A, Hirohama M, Nagata K.
  • Organizer: The 16th International Negative Strand Virus Meeting
  • Place of Presentation: シエナ大学（シエナ）
  • Year and Date: 2015-06-15
  • Int'l Joint Research
• [Presentation] 中心体の機能制御を介したインフルエンザウイルスゲノムの細胞内輸送機構 (2014)
  • Author(s): 川口敦史、広浜美香子、永田恭介
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] Host factor-dependent influenza virus genome replication (2014)
  • Author(s): Nagata K.
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10 – 2014-11-12
  • Invited
• [Presentation] インフルエンザウイルスポリメラーゼサブユニット間の適合性 (2014)
  • Author(s): 原田芳美、永田恭介、川口敦史
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] インフルエンザウイルス粒子形成におけるアクトミオシンネットワークの機能 (2014)
  • Author(s): 熊倉充子、川口敦史、永田恭介
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] インフルエンザウイルスcell-to-cell感染によるウイルスゲノムの多様化 (2014)
  • Author(s): 森幸太郎、川口敦史、村野健作、永田恭介
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules for the virus production. (2013)
  • Author(s): Kawaguchi A.
  • Organizer: The 2nd Meeting on RNA and Biofunctions-Asis Study "RNA Biofunctions and Viruses"
  • Place of Presentation: Fukuoka
  • Year and Date: 2013-01-09
• [Presentation] Host factor-dependent RNA replication of the influenza virus genome. (2013)
  • Author(s): 永田恭介
  • Organizer: The 12th Awaji International Forum on Infection and Immunity.
  • Place of Presentation: 淡路
  • Invited
• [Presentation] インフルエンザウイルスゲノムの機能発現機構と創薬展開 (2013)
  • Author(s): 永田恭介
  • Organizer: 第66回日本細菌学会九州支部総会・第50回日本ウイルス学会九州支部総会
  • Place of Presentation: 長崎
  • Invited
• [Presentation] Tamiflu resistant cell-to-cell transmission of cell associated influenza virus (2013)
  • Author(s): 森幸太郎、春山貴弘、永田恭介
  • Organizer: The 12th Awaji International Forum on Infection and Immunity.
  • Place of Presentation: 淡路
• [Presentation] インフルエンザポリメラーゼ遺伝子間の適合性 (2013)
  • Author(s): 原田芳美、川口敦史、永田恭介
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] インフルエンザウイルスゲノムの細胞内輸送機構におけるNS1の新規機能 (2013)
  • Author(s): 長利卓、川口敦史、永田恭介
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] インフルエンザウイルス増殖にかかわる細胞骨格関連因子 (2013)
  • Author(s): 熊倉充子、永田恭介
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] インフルエンザウイルスにおけるNA非依存的cell-to-cell感染機構 (2013)
  • Author(s): 森幸太郎、春山貴弘、永田恭介
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] インフルエンザウイルス感染に応答した宿主因子YB-1による中心体の機能制御 (2013)
  • Author(s): 川口敦史、永田恭介
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] rRNA遺伝子の種特異的転写機構の解析 (2013)
  • Author(s): 村野健作、永田恭介
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] ポリコーム複合体因子であるEZH2はインフルエンザウイルスタンパク質M1およびインフルエンザウイルスゲノムの核外輸送を制御する (2013)
  • Author(s): 浅賀正充、川口敦史、永田恭介
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] M期リン酸化によるCTCFのDNA結合活性制御の解析 (2013)
  • Author(s): 関屋健史、村野健作、永田恭介
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 薬剤耐性ウイルスが出現しない抗インフルエンザウイルス薬開発に向けた宿主因子依存性ウイルスRNAゲノムの複製転写機構． (2012)
  • Author(s): 永田恭介
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] RNA依存性RNAポリメラーゼを用いた新規レポーターシステムによるrRNA遺伝子の種特異的転写機構の解析． (2012)
  • Author(s): 村野健作
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] 細胞がん化に関わる核膜孔タンパク質の発現異常． (2012)
  • Author(s): 齋藤祥子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] 新規宿主因子YB-1によるインフルエンザウイルスRNP複合体の微小管への輸送機構解析． (2012)
  • Author(s): 川口敦史
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] VprBP, a Substrate Receptor Subunit of Cullin4-DDB1 E3 Ubiquitin Ligase Complex, is Involved Ubiquitination of Histone H1. (2012)
  • Author(s): 加藤広介
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] EZH2はインフルエンザゲノムの核外輸送を制御する． (2012)
  • Author(s): 浅賀正充
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] ヒト化マウスを用いた麻疹ウイルス感染モデルマウスの作製． (2012)
  • Author(s): 木村英樹
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-13
• [Presentation] インフルエンザウイルスRNAポリメラーゼの伸長活性に及ぼすキャップ構造の意義． (2012)
  • Author(s): 若井ちとせ
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-13
• [Presentation] 細胞骨格関連因子によるインフルエンザウイルス増殖制御． (2012)
  • Author(s): 熊倉充子
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-13
• [Presentation] 新規宿主因子YB-1によるインフルエンザウイルスゲノムの細胞内輸送制御． (2012)
  • Author(s): 川口敦史
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-13
• [Presentation] YB-1 is a porter to lead influenza virus ribonucleoprotein complexes to microtubules. (2012)
  • Author(s): Kawaguchi A.
  • Organizer: The 3rd Leading Graduate Schools International Conference (Tsukuba Global Science Week)
  • Place of Presentation: Tsukuba
  • Year and Date: 2012-11-01
• [Presentation] esign of a novel artificial metallonuclease for targeting the breakpoint in Duchenne Muscular Dystrophy. (2012)
  • Author(s): Gyurcsik B.
  • Organizer: The 3rd Leading Graduate Schools International Conference (Tsukuba Global Science Week)
  • Place of Presentation: Tsukuba
  • Year and Date: 2012-11-01
• [Presentation] The Species-specific transcription mechanism of rRNA gene revealed by a novel monitoring system-using RNA-dependent RNA polymerase. (2012)
  • Author(s): Murano K.
  • Organizer: The 7th Tsukuba Medical Science Research Meeting (Tsukuba Global Science Week)
  • Place of Presentation: Tsukuba
  • Year and Date: 2012-11-01
• [Presentation] The promoter binding sites in the influenza virus polymerase PB1. (2012)
  • Author(s): Binh N.T.
  • Organizer: The 7th Tsukuba Medical Science Research Meeting (Tsukuba Global Science Week)
  • Place of Presentation: Tsukuba
  • Year and Date: 2012-11-01
• [Presentation] Function of IREF-2, a newly identified for replication of the influenza virus RNA genome. (2012)
  • Author(s): Sugiyama K.
  • Organizer: The 7th Tsukuba Medical Science Research Meeting (Tsukuba Global Science Week)
  • Place of Presentation: Tsukuba
  • Year and Date: 2012-11-01
• [Presentation] A novel function of NS1 viral protein in intracellular of the influenza virus genome. (2012)
  • Author(s): Osari S.
  • Organizer: The 7th Tsukuba Medical Science Research Meeting (Tsukuba Global Science Week)
  • Place of Presentation: Tsukuba
  • Year and Date: 2012-11-01
• [Presentation] Assembly and maintenance of the nucleolar structure by RNA and RNA binding protein. (2012)
  • Author(s): Okuwaki M.
  • Organizer: Human Biology Symposium-Biology and Regenerative Medicine
  • Place of Presentation: Tsukuba
  • Year and Date: 2012-10-30
• [Presentation] ヒストンH1シャペロンTAF-Iによるクロマチン動態制御機構の解析． (2012)
  • Author(s): 加藤広介
  • Organizer: 生殖サイクル若手勉強会2012
  • Place of Presentation: 仙台
  • Year and Date: 2012-07-25
• [Presentation] ウイルス研究が育んだ現代生命科学． (2012)
  • Author(s): 永田恭介
  • Organizer: 第9回ウイルス学キャンプin湯河原
  • Place of Presentation: 熱海
  • Year and Date: 2012-07-10
• [Presentation] インフルエンザウイルスゲノムの細胞内輸送機構の解析． (2012)
  • Author(s): 川口敦史
  • Organizer: 第9回ウイルス学キャンプin湯河原
  • Place of Presentation: 熱海
  • Year and Date: 2012-07-10
• [Presentation] インフルエンザウイルスゲノムの輸送にかかわる細胞骨格関連因子の解析． (2012)
  • Author(s): 熊倉充子
  • Organizer: 第9回ウイルス学キャンプin湯河原
  • Place of Presentation: 熱海
  • Year and Date: 2012-07-10
• [Presentation] NS1によるインフルエンザウイルスゲノムの細胞内局在決定機構の解析． (2012)
  • Author(s): 長利卓
  • Organizer: 第9回ウイルス学キャンプin湯河原
  • Place of Presentation: 熱海
  • Year and Date: 2012-07-10
• [Presentation] ウイルスポリメラーゼによって規定されるトリインフルエンザウイルスの哺乳類細胞への適応機構
  • Author(s): 原田芳美、 川口敦史、 永田恭介
  • Organizer: 日本生化学会関東支部例会
  • Place of Presentation: 甲府
• [Presentation] インフルエンザウイルスゲノムの複製機構に基盤をおいた創薬
  • Author(s): 永田恭介
  • Organizer: 第42回ヒューマンサイエンス総合研究セミナー
  • Place of Presentation: 東京
• [Remarks] 筑波大学医学医療系分子ウイルス学
  • URL: http://www.md.tsukuba.ac.jp/basic-med/infectionbiology/virology/
• [Remarks] ウイルス感染現象における宿主細胞コンピテンシーの分子基盤
  • URL: http://www.md.tsukuba.ac.jp/basic-med/infcompetence/
• [Remarks] 筑波大学 分子ウイルス学分野
  • URL: http://www.md.tsukuba.ac.jp/basic-med/infectionbiology/virology/
• [Remarks] 筑波大学 感染生物学分子ウイルス学分野
  • URL: http://www.md.tsukuba.ac.jp/basic-med/infectionbiology/virology/