|Budget Amount *help
¥143,130,000 (Direct Cost: ¥110,100,000、Indirect Cost: ¥33,030,000)
Fiscal Year 2016: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2015: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2014: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2013: ¥28,080,000 (Direct Cost: ¥21,600,000、Indirect Cost: ¥6,480,000)
Fiscal Year 2012: ¥34,320,000 (Direct Cost: ¥26,400,000、Indirect Cost: ¥7,920,000)
|Outline of Final Research Achievements
This research aimed to identify host factors, which regulate influenza virus genome function, and to analyze the replicational and post-replicational regulation of influenza virus genome.
We identified pp32/APRIL and Prp18 as stimulatory factors of viral RNA polymerase. Further, we found that newly synthesized viral genomes are recruited to the centrosome by YB-1, and the progeny viral genome is transported to the plasma membrane via cholesterol-enriched Rab11a-positive recycling endosomes. Cholesterol is thought to be a trigger for the virus assembly concomitantly with arrival of the virus genome beneath the plasma membrane.