| Budget Amount *help |
¥86,970,000 (Direct Cost: ¥66,900,000、Indirect Cost: ¥20,070,000)
Fiscal Year 2016: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2015: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2014: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2013: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2012: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
|
|---|
| Outline of Final Research Achievements |
In bacteria, protein translocation is mediated by two repetitive motors, SecA ATPase and SecDF, which exist on the cytoplasmic side and periplasic side of the SecYEG channel, respectively. In order to elucidate how these two motors contribute to protein translocation reaction, we carried out the following experiments. 1) We systematically performed site-directed in vivo photo cross-linking experiments targeted to E. coli SecD and identified a substrate binding site in the SecD protein. 2) We determined new crystal structures of SecDF in collaboration with Dr. Tsukazaki and carried out structure-instructed biochemical studies. 3) We determined biochemical properties and physiological functions of two SecDF paralogs in Vibrio alginolyticus.
|
