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Structure and dynamics of gliding machinery in Bacteroidetes phylum bacteria

Planned Research

Project AreaHarmonized supramolecular machinery for motility and its diversity
Project/Area Number 24117006
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNagasaki University

Principal Investigator

NAKAYAMA Koji  長崎大学, 医歯薬学総合研究科(歯学系), 教授 (80150473)

Co-Investigator(Kenkyū-buntansha) 佐藤 啓子  長崎大学, 医歯薬学総合研究科(歯学系), 助教 (70410579)
Project Period (FY) 2012-06-28 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥93,340,000 (Direct Cost: ¥71,800,000、Indirect Cost: ¥21,540,000)
Fiscal Year 2016: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2015: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2014: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2013: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2012: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Keywords運動 / 細菌 / 蛋白質分泌 / タンパク質分泌 / 滑走運動 / 分泌機構
Outline of Final Research Achievements

Many of the Bacteroidetes phylum bacteria such as Flavobacterium johnsoniae exhibit rapid gliding motility on the substratum by a unique mechanism. This gliding motility relies on novel gliding machinery consisting of Gld and Spr proteins and requires SprB adhesin. SprB is a thin filament of 150 nm length extending from the cell surface, it is thought that SprB movement on the bacterial body along the closed spiral loop and attachment to the substratum cause rotation and translation of the bacterial cell. The multi-rail structure by GldJ is on the inner surface of the outer membrane, and gliding motility is supposed to occur by the movement of SprB adhesin along this structure.

Report

(6 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (26 results)

All 2017 2016 2015 2014 2013 2012 Other

All Int'l Joint Research (3 results) Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 12 results,  Open Access: 7 results,  Acknowledgement Compliant: 4 results) Presentation (11 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results)

  • [Int'l Joint Research] メルボルン大学(オーストラリア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] SLAC National Accelerator Laboratory/The Scripps Research Institute(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Queen Mary University of London(英国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Structural Insights into the PorK and PorN Components of the Porphyromonas gingivalis Type IX Secretion System.2016

    • Author(s)
      Gorasia DG, Veith PD, Hanssen EG, Glew MD, Sato K, Yukitake H, Nakayama K, Reynolds EC
    • Journal Title

      PLoS Pathog

      Volume: 12 Issue: 8 Pages: e1005820-e1005820

    • DOI

      10.1371/journal.ppat.1005820

    • NAID

      120006987659

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The complete genome sequencing of Prevotella intermedia strain OMA14 and a subsequent finescale, intra-species genomic comparison reveal an unusual amplification of conjugative and mobile transposons and identify a novel Prevotella-lineage-specific repeat.2016

    • Author(s)
      Mariko Naito, Yoshitoshi Ogura, Takehiko Itoh, Mikio Shoji, Masaaki Okamoto, Tetsuya Hayashi, and Koji Nakayama
    • Journal Title

      DNA Research

      Volume: 23 Pages: 11-16

    • DOI

      10.1093/dnares/dsv032

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Distinct Type of Pilus from the Human Microbiome.2016

    • Author(s)
      Xu Q, Shoji M, Shibata S, Naito M, Sato K, Elsliger MA, Grant JC, Axelrod HL, Chiu HJ, Farr CL, Jaroszewski L, Knuth MW, Deacon AM, Godzik A, Lesley SA, Curtis MA, Nakayama K, Wilson IA
    • Journal Title

      Cell

      Volume: 165 Issue: 3 Pages: 690-703

    • DOI

      10.1016/j.cell.2016.03.016

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A two-component system regulates gene expression of the type IX secretion component proteins via an ECF sigma factor2016

    • Author(s)
      門脇知子, 雪竹英治, 内藤真理子, 佐藤啓子, 菊池有一郎, 近藤好夫, 庄子幹郎, 中山浩次
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 23288-23288

    • DOI

      10.1038/srep23288

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of the type IX secretion system in the gliding motility and biofilm formation of Capnocytophaga ochracea2016

    • Author(s)
      Kita D, Shibata S, Kikuchi Y, Kokubu E, Nakayama K, Saito A, Ishihara K
    • Journal Title

      Appl Environ Microbiol

      Volume: 82 Issue: 6 Pages: 1756-1766

    • DOI

      10.1128/aem.03452-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Porphyromonas gingivalis and related bacteria: from colonial pigmentation to the type IX secretion system and gliding motility.2015

    • Author(s)
      Nakayama K
    • Journal Title

      J Periodontal Res

      Volume: 50 Issue: 1 Pages: 1-8

    • DOI

      10.1111/jre.12255

    • NAID

      120006986253

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Involvement of the Wbp pathway in the biosynthesis of Porphyromonas gingivalis lipopolysaccharide with anionic polysaccharide.2014

    • Author(s)
      6.Shoji M, Sato K, Yukitake H, Naito M, Nakayama K
    • Journal Title

      Scientific Reports

      Volume: 4 Pages: 5056-5056

    • NAID

      120006986576

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Lack of a surface layer in Tannerella forsythia mutants deficient in the type IX secretion system.2014

    • Author(s)
      7.Narita Y, Sato K, Yukitake H, Shoji M, Daisuke N, Nagano K, Yoshimura F, Naito M, Nakayama K
    • Journal Title

      Microbiology-SGM

      Volume: 160 Issue: 10 Pages: 2295-2303

    • DOI

      10.1099/mic.0.080192-0

    • NAID

      120006986357

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Analysis of a Lys-specific serine endopeptidase secreted via the type IX secretion system in Porphyromonas gingivalis.2014

    • Author(s)
      Nonaka M, Shoji M, Kadowaki T, Sato K, Yukitake H, Naito M, Nakayama K
    • Journal Title

      FEMS Microbiol Lett.

      Volume: 354 Pages: 60-68

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Helical flow of surface protein required for bacterial gliding motility.2013

    • Author(s)
      102. Nakane D, Sato K, Wada H, McBride MJ, Nakayama K
    • Journal Title

      Proc. Nat. Acd. Sci. USA

      Volume: 110 Issue: 27 Pages: 11145-11150

    • DOI

      10.1073/pnas.1219753110

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of an O-antigen chain length regul tor, WzzP, in Porphyromonas gingivalis.2013

    • Author(s)
      Shoji M, Yukitake H, Sato K, Shibata Y, Naito M, Aduse-Opoku J, Abiko Y, Curtis MA, N kayama K.
    • Journal Title

      Microbiologyopen.Epub

      Volume: 10 Issue: 3 Pages: 383-401

    • DOI

      10.1002/mbo3.84

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of Porphyromonas gingivalis proteins secreted by the Por secretion system.2013

    • Author(s)
      Sato K, Yukitake H, Narita Y, Shoji M, Naito M, Nakayama K.
    • Journal Title

      FEMS microbiology Letters

      Volume: 338 (1) Issue: 1 Pages: 68-76

    • DOI

      10.1111/1574-6968.12028

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] バクテロイデーテス門細菌のIX型分泌機構およびV型線毛の研究2017

    • Author(s)
      中山浩次
    • Organizer
      日本細菌学会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] The type IX secretion, gliding motility and the type V pilus2016

    • Author(s)
      Nakayama K
    • Organizer
      Protein Secretion in Bacteria Conference
    • Place of Presentation
      シラタ・ビーチ・リゾート・ホテル(米国フロリダ州タンパ)
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Type IX secretion system and gliding motility in Bacteroidetes phylum bacteria2016

    • Author(s)
      Nakayama K
    • Organizer
      The 13th Korea-Japan International Symposium on Microbiology
    • Place of Presentation
      K ホテル(韓国慶州市)
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細菌の新しい分泌システムと滑走運動2015

    • Author(s)
      中山浩次
    • Organizer
      第98回日本細菌学会関東支部総会
    • Place of Presentation
      東京歯科大学(東京都千代田区)
    • Year and Date
      2015-10-29
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 滑走細菌Flavobacterium johnsoniaeのレール状滑走装置2015

    • Author(s)
      柴田 敏史, 川本 晃大, 加藤 貴之, 難波 啓一, 中山 浩次
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学(石川県金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] The type IX secretion system of Porphyromonas gingivalis2015

    • Author(s)
      Nakayama K
    • Organizer
      PgLONDON2015
    • Place of Presentation
      ロンドン(イギリス)
    • Year and Date
      2015-06-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Flavobacterium johnsoniaeのレール状滑走装置2015

    • Author(s)
      柴田敏史、中山浩次
    • Organizer
      2015年生体運動合同班会議
    • Place of Presentation
      学習院大学(東京都豊島区)
    • Year and Date
      2015-01-07 – 2015-01-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 滑走細菌Flavobacterium johnsoniaeの菌体表面の滑走運動装置2014

    • Author(s)
      柴田敏史、佐藤啓子、成田由香、中根大介、中山浩次
    • Organizer
      日本細菌学会
    • Place of Presentation
      東京都江戸川区タワーホール船堀
    • Related Report
      2013 Annual Research Report
  • [Presentation] Helical flow of surface protein required for Flavobacterium gliding motility.2013

    • Author(s)
      Nakane D, Sato K, Wada H, McBride MJ, Nakayama K
    • Organizer
      BLAST XII
    • Place of Presentation
      Tucson, AZ, USA
    • Year and Date
      2013-01-20
    • Related Report
      2012 Annual Research Report
  • [Presentation] Secretion and gliding of Bacteroidetes phylum.2013

    • Author(s)
      Sato K, Nakane D, McBride MJ, Nakayama K
    • Organizer
      BLAST XII
    • Place of Presentation
      Tucson, AZ, USA
    • Year and Date
      2013-01-20
    • Related Report
      2012 Annual Research Report
  • [Presentation] Helical flow of surface protein required for Bacteroidetes gliding motility.2012

    • Author(s)
      Nakane D, Sato K, Wada H, McBride MJ, Nakayama K
    • Organizer
      First International Conference on Porphyromonas gingivalis and Related Bacterial Species
    • Place of Presentation
      Nagasaki, Japan
    • Year and Date
      2012-08-27
    • Related Report
      2012 Annual Research Report

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Published: 2012-11-27   Modified: 2022-02-07  

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