Regulation mechanism of a transcription cycle by static/dynamic structural analyses

• Project Area: Integral understanding of the mechanism of transcription cycle through quantitative, high-resolution approaches
• Project/Area Number: 24118005
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Yokohama City University
• Principal Investigator: OGATA Kazuhiro 横浜市立大学, 医学研究科, 教授 (90260330)
• Co-Investigator(Renkei-kenkyūsha): SHIINA Masaaki 横浜市立大学, 医学部, 助教 (30347299) ; HAMADA Keisuke 横浜市立大学, 医学部, 助教 (00344052)
• Project Period (FY): 2012-06-28 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥122,330,000 (Direct Cost: ¥94,100,000、Indirect Cost: ¥28,230,000); Fiscal Year 2016: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000); Fiscal Year 2015: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000); Fiscal Year 2014: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000); Fiscal Year 2013: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000); Fiscal Year 2012: ¥28,600,000 (Direct Cost: ¥22,000,000、Indirect Cost: ¥6,600,000)
• Keywords: 転写制御 / 転写因子 / 化学修飾 / Ｘ線結晶構造解析 / エンハンソソーム / 細胞シグナル / 分子動力学 / 発現制御 / タンパク質 / Ｘ線結晶構造 / Runx1 / Ets1 / Nrf2 / リン酸化 / X線結晶構造解析 / TCR / LEF1

Outline of Final Research Achievements

Signal transduction transfers cellular environmental information to nucleus via chemical modifications of proteins and finally modulates transcription profile of the cell. To reveal the molecular mechanism of conversion of the information from chemical modification to transcriptional modulation, we employed static and dynamic structural analyses of higher-ordered transcription factors(TF)-DNA complexes. We found that phosphorylation of the intrinsically disordered region of the transcription factor Ets1 induces a non-DNA-binding conformer of Ets1 itself. On the tcrα enhancer, the partner TF Runx1 selects the specific Ets1 conformer that is refractory to effect of phosphorylation, resisting the inhibitory effect of phosphorylation of Ets1 and specifically keeping the tcrα gene transcription active, while many of other Ets1-target genes turn off. This transcription switch may enable fine regulation responding to cellular environments including development and proliferation signals.

Research Products

• [Journal Article] A novel GFI1B mutation at the first zinc finger domain causes congenital macrothrombocytopenia (2017)
  • Author(s): Uchiyama Y, Ogawa Y, Kunishima S, Shiina M, Nakashima M, Yanagisawa K, Yokohama A, Imagawa E, Miyatake S, Mizuguchi T, Takata A, Miyake N, Ogata K, Handa H, Matsumoto N
  • Journal Title: British Journal of Haematology Volume: 印刷中 Issue: 6 Pages: 843-847
  • DOI: 10.1111/bjh.14710
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular mechanisms of cooperative binding of transcription factors Runx1-CBFβ-Ets1 on the TCRα gene enhancer (2017)
  • Author(s): Kasahara K, Shiina M, Fukuda I, Ogata K, Nakamura H
  • Journal Title: PloS One Volume: 12 Issue: 2 Pages: e0172654-e0172654
  • DOI: 10.1371/journal.pone.0172654
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Biallelic mutations in the 3' exonuclease TOE1 cause pontocerebellar hypoplasia and uncover a role in snRNA processing (2017)
  • Author(s): Lardelli RM, Schaffer AE, Eggens VR, ... Matsumoto N, Muramatsu K, Saitsu H, Shiina M, Ogata K, Foulds N, Dobyns WB, Chi NC, Traver D, Spaccini L, Bova SM, Gabriel SB, Gunel M, Valente EM, Nassogne MC, Bennett EJ, Yeo GW, Baas F, Lykke-Andersen J, Gleeson JG
  • Journal Title: Nature Genetics Volume: 49 Issue: 3 Pages: 457-464
  • DOI: 10.1038/ng.3762
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] PARS2 and NARS2 mutations in infantile-onset neurodegenerative disorder (2017)
  • Author(s): Mizuguchi T, Nakashima M, Kato M, Yamada K, Okanishi T, Ekhilevitch N, Mandel H, Eran A, Toyono M, Sawaishi Y, Motoi H, Shiina M, Ogata K, Miyatake S, Miyake N, Saitsu H, Matsumoto N
  • Journal Title: Journal of Human Genetics Volume: 62 Issue: 5 Pages: 525-529
  • DOI: 10.1038/jhg.2016.163
  • NAID: 40021210259
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Biallelic mutations in MYPN, encoding Myopalladin, are associated with childhood-onset, slowly progressive nemaline myopathy (2017)
  • Author(s): Miyatake S, Mitsuhashi S, Hayashi YK, Purevjav E, Nishikawa A, Koshimizu E, Suzuki M, Yatabe K, Tanaka Y, Ogata K, Kuru S, Shiina M, Tsurusaki Y, Nakashima M, Mizuguchi T, Miyake N, Saitsu H, Ogata K, Kawai M, Towbin J, Nonaka I, Nishino I, Matsumoto N
  • Journal Title: American Journal of Human Genetics Volume: 100 Issue: 1 Pages: 169-178
  • DOI: 10.1016/j.ajhg.2016.11.017
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Distal arthrogryposis with variable clinical expression caused by TNNI2 mutation (2017)
  • Author(s): Culic V, Miyake N, Jankovic S, Petrovic D, Simunovic M, Dapic T, Shiina M, Ogata K, Matsumoto N
  • Journal Title: Human Genome Variation Volume: 3 Issue: 1 Pages: 16035-16035
  • DOI: 10.1038/hgv.2016.35
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The first report of Japanese patients with asparagine synthetase deficiency (2017)
  • Author(s): Yamamoto T, Endo W, Ohnishi H, Kubota K, Kawamoto N, Inui T, Imamura A, Takanashi JI, Shiina M, Saitsu H, Ogata K, Matsumoto N, Haginoya K, Fukao T
  • Journal Title: Brain Development Volume: 39 Issue: 3 Pages: 236-242
  • DOI: 10.1016/j.braindev.2016.09.010
  • Peer Reviewed
• [Journal Article] Biallelic TBCD mutations cause early-onset neurodegenerative encephalopathy (2017)
  • Author(s): Miyake N, Fukai R, Ohba C, ... Shiina M, Ogata K, Okuno-Yuguchi J, Fueki N, Ogiso Y, Suzumura H, Watabe Y, Imataka G, Leong HY, Fattal-Valevski A, Kramer U, Miyatake S, Kato M, Okamoto N, Sato Y, Mitsuhashi S, Nishino I, Kaneko N, Nishiyama A, Tamura T, Mizuguchi T, Nakashima M, Tanaka F, Saitsu H, Matsumoto N
  • Journal Title: American Journal of Human Genetics Volume: 99 Issue: 4 Pages: 950-961
  • DOI: 10.1016/j.ajhg.2016.08.005
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phenotypic spectrum of GNAO1 variants: epileptic encephalopathy to involuntary movements with severe developmental delay (2016)
  • Author(s): Saitsu H, Fukai R, Ben-Zeev B, Sakai Y, Mimaki M, Okamoto N, Suzuki Y, Monden Y, Saito H, Tziperman B, Torio M, Akamine S, Takahashi N, Osaka H, Yamagata T, Nakamura K, Tsurusaki Y, Nakashima M, Miyake N, Shiina M, Ogata K, Matsumoto N
  • Journal Title: Eur J Hum Genet Volume: 24(1) Issue: 1 Pages: 129-134
  • DOI: 10.1038/ejhg.2015.92
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Biallelic mutations in nuclear pore complex subunit NUP107 cause early-childhood-onset steroid-resistant nephrotic syndrome (2015)
  • Author(s): Miyake N, Tsukaguchi H, Koshimizu E, Shono A, Matsunaga S, Shiina M, Mimura Y, Imamura S, Hirose T, Okudela K, Nozu K, Akioka Y, Hattori M, Yoshikawa N, Kitamura A, Cheong HI, Kagami S, Yamashita M, Fujita A, Miyatake S, Tsurusaki Y, Nakashima M, Saitsu H, Ohashi K, Imamoto N, Ryo A, Ogata K, Iijima K, Matsumoto N
  • Journal Title: Am J Hum Genet Volume: 97(4) Issue: 4 Pages: 555-566
  • DOI: 10.1016/j.ajhg.2015.08.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of HOXD4 Mutations in Spinal Extradural Arachnoid Cyst (2015)
  • Author(s): Ogura Y, Miyake N, Kou I, Iida A, Nakajima M, Takeda K, Fujibayashi S, Shiina M, Okada E, Toyama Y, Iwanami A, Ishii K, Ogata K, Asahara H, Matsumoto N, Nakamura M, Matsumoto M, Ikegawa S
  • Journal Title: PLoS One Volume: 10(11) Issue: 11 Pages: e0142126-e0142126
  • DOI: 10.1371/journal.pone.0142126
  • NAID: 120005763410
  • Peer Reviewed / Open Access
• [Journal Article] Short-lasting unilateral neuralgiform headache attacks with ispilateral facial flushing is a new variant of paroxysmal extreme pain disorder (2015)
  • Author(s): Imai N, Miyake N, Saito Y, Kobayashi E, Ikawa M, Manaka S, Shiina M, Ogata K, Matsumoto N
  • Journal Title: J Headache Pain Volume: 16(1) Issue: 1 Pages: 519-519
  • DOI: 10.1186/s10194-015-0519-3
  • Peer Reviewed / Open Access
• [Journal Article] GRIN1 mutations cause encephalopathy with infantile-onset epilepsy, and hyperkinetic and stereotyped movement disorders (2015)
  • Author(s): Ohba C, Shiina M, Tohyama J, Haginoya K, Lerman-Sagie T, Okamoto N, Blumkin L, Lev D, Mukaida S, Nozaki F, Uematsu M, Onuma A, Kodera H, Nakashima M, Tsurusaki Y, Miyake N, Tanaka F, Kato M, Ogata K, Saitsu H, Matsumoto N
  • Journal Title: Epilepsia Volume: 印刷中 Issue: 6 Pages: 841-848
  • DOI: 10.1111/epi.12987
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Somatic Mutations in the MTOR gene cause focal cortical dysplasia type IIb (2015)
  • Author(s): Nakashima M, Saitsu H, Takei N, Tohyama J, Kato M, Kitaura H, Shiina M, Shirozu H, Masuda H, Watanabe K, Ohba C, Tsurusaki Y, Miyake N, Zheng Y, Sato T, Takebayashi H, Ogata K, Kameyama S, Kakita A, Matsumoto N
  • Journal Title: Ann. Neurol. Volume: 78 Issue: 3 Pages: 375-386
  • DOI: 10.1002/ana.24444
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A novel allosteric mechanism on protein-DNA interactions underlying the phosphorylation-dependent regulation of Ets1 target gene expressions (2015)
  • Author(s): Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Uchiyama A, Baba S, Sato K, Yamamoto M, Ogata K
  • Journal Title: J Mol Biol Volume: 427(8) Issue: 8 Pages: 1655-1669
  • DOI: 10.1016/j.jmb.2014.07.020
  • Peer Reviewed / Open Access
• [Journal Article] Novel compound heterozygous LIAS mutations cause glycine encephalopathy (2015)
  • Author(s): Tsurusaki Y, Tanaka R, Shimada S, Shimojima K, Shiina M, Nakashima M, Saitsu H, Miyake N, Ogata K, Yamamoto T, Matsumoto N
  • Journal Title: J Hum Genet Volume: 60(10) Issue: 10 Pages: 631-635
  • DOI: 10.1038/jhg.2015.72
  • Peer Reviewed / Open Access
• [Journal Article] De novo DNM1 mutations in two cases of epileptic encephalopathy. (2015)
  • Author(s): Nakashima M, Kouga T, Lourenço CM, Shiina M, Goto T, Tsurusaki Y, Miyatake S, Miyake N, Saitsu H, Ogata K, Osaka H, Matsumoto N.
  • Journal Title: Epilepsia Volume: Epub 2015 Nov 27. Issue: 1
  • DOI: 10.1111/epi.13257
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Crystallization of the Ets1-Runx1-CBFβ-DNA complex formed on the TCRα gene enhancer (2014)
  • Author(s): Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Baba S, Sato K, Ogata K
  • Journal Title: Acta Crystallogr F Struct Biol Commun Volume: 70 Issue: 10 Pages: 1380-1384
  • DOI: 10.1107/s2053230x14018470
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] De novo SOX11 mutations cause Coffin-Siris syndrome (2014)
  • Author(s): Tsurusaki Y, Ohashi H, Phadke S, Koshimizu E, Kou I, Shiina M, Suzuki T, Okamoto N, Imamura S, Yamashita M, Watanabe S, Yoshiura K-i, Kodera H, Miyatake S, Nakashima N, Saitsu H, Ogata K, Ikegawa S, Miyake N, and Matsumoto N.
  • Journal Title: Nat Commun Volume: 5 Issue: 1 Pages: 4011-4011
  • DOI: 10.1038/ncomms5011
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Deep sequencing detects very low-grade somatic mosaicism in the unaffected mother of siblings with nemaline myopathy (2014)
  • Author(s): Miyatake S, Koshimizu E, Hayashi YK, Miya K, Shiina M, Nakashima M, Tsurusaki Y, Miyake N, Saitsu H, Ogata K, Nishino I, Matsumoto N.
  • Journal Title: Neuromuscul Disord Volume: 24（7） Issue: 7 Pages: 642-647
  • DOI: 10.1016/j.nmd.2014.04.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Expanding the phenotypic spectrum of TUBB4A-associated (2014)
  • Author(s): Miyatake S, Osaka H, Shiina M, Sasaki M, Takanashi J, Haginoya K, Wada T,
  • Journal Title: Neurology Volume: 82(24) Issue: 24 Pages: 2230-7
  • DOI: 10.1212/wnl.0000000000000535
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A hemizygous GYG2 mutation and Leigh syndrome: a possible link? (2014)
  • Author(s): Imagawa E, Osaka H, Yamashita A, Shiina M, Takahashi E, Sugie H, Nakashima M, Tsurusaki Y, Saitsu H, Ogata K, Matsumoto N, Miyake N.
  • Journal Title: Hum Genet. Volume: 133(2 Issue: 2 Pages: 225-34
  • DOI: 10.1007/s00439-013-1372-6
  • Peer Reviewed / Open Access
• [Journal Article] Late-onset spastic ataxia phenotype in a patient with a homozygous DDHD2 mutation (2014)
  • Author(s): Doi H, Ushiyama M, Baba T, Tani K, Shiina M, Ogata K, Miyatake S, Yuzawa YF, Tsuji S, Nakashima M, Tsurusaki Y, Miyake N, Saitsu H, Ikeda S, Tanaka F, Matsumoto N, Yoshida K
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 7132-7132
  • DOI: 10.1038/srep07132
  • NAID: 120007100602
  • Peer Reviewed / Open Access
• [Journal Article] De novo SOX11 mutations cause Coffin-Siris syndrome (2014)
  • Author(s): Y Tsurusaki, H Ohashi, S Phadke, E Koshimizu, I Kou, M Shiina, T Suzuki, N Okamoto, S Imamura, M Yamashita, S Watanabe, K Yoshiura, H Kodera, S Miyatake, M Nakashima, H Saitsu, K Ogata, S Ikegawa, N Miyake, N Matsumoto
  • Journal Title: Nat Commun Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Mitochondrial complex III deficiency caused by a homozygous UQCRC2 mutation presenting with neonatal-onset recurrent metabolic decompensation (2013)
  • Author(s): Miyake N^<#>, Yano S^<#> (# denotes equal contribution), Sakai C, Hatakeyama H, Shiina M, Watanabe Y, Bartley J, Abdenur JE, Wang RY, Chang R, Tsurusaki Y, Doi H, Saitsu H, Ogata K, Goto Y, *Matsumoto N
  • Journal Title: Hum Mut Volume: 34(3):446-452 Issue: 3 Pages: 446-452
  • DOI: 10.1002/humu.22257
  • Peer Reviewed
• [Journal Article] Exome sequencing identifies a novel INPPL1 mutation in opsismodysplasia (2013)
  • Author(s): Iida A, Okamoto N, Miyake N, Nishimura G, Minami S, Sugimoto T, Nakashima M, Tsurusaki Y, Saitsu H, Shiina M, Ogata K, Watanabe S, Ohashi H, Matsumoto N, Ikegawa S
  • Journal Title: J Hum Genet Volume: 58 Issue: 6 Pages: 391-394
  • DOI: 10.1038/jhg.2013.25
  • NAID: 10031184228
  • Peer Reviewed
• [Journal Article] Identification of KLHL41 mutations implicates BTB-Kelch-mediated ubiquitination as an alternate pathway to myofibrillar disruption in nemaline myopathy (2013)
  • Author(s): Gupta V, Shiina M, Ogata K, Matsumoto N, Beggs N et al.
  • Journal Title: Am J Hum Genet Volume: 93 Issue: 6 Pages: 1108-1117
  • DOI: 10.1016/j.ajhg.2013.10.020
  • Peer Reviewed
• [Journal Article] De Novo Mutations in GNAO1, Encoding a Gαo Subunit of Heterotrimeric G Proteins, Cause Epileptic Encephalopathy. (2013)
  • Author(s): Nakamura, K., et al.,
  • Journal Title: Am. J. Hum. Genet. Volume: 93 Issue: 3 Pages: 496-505
  • DOI: 10.1016/j.ajhg.2013.07.014
  • Peer Reviewed
• [Journal Article] KDM6A point mutations cause Kabuki syndrome (2012)
  • Author(s): Miyake N, Mizuno S, Okamoto N, Ohashi H, Shiina M, Ogata K, Tsurusaki Y, Nakashima M, Saitsu H, *Matsumoto N (*: co-corresponding)
  • Journal Title: Hum Mut Volume: 34(1):108-110 Issue: 1 Pages: 108-110
  • DOI: 10.1002/humu.22229
  • Peer Reviewed
• [Journal Article] Mutations affecting components of the SWI/SNF complex cause Coffin-Siris syndrome (2012)
  • Author(s): Tsurusaki Y, Okamoto N, Ohashi H, Kosho T, Imai Y, Hibi-Ko Y, Kaname T, Naritomi K, Kawame H, Wakui K, Fukushima Y, Homma T, Kato M, Hiraki Y, Yamagata T, Yano S, Mizuno S, Sakazume S, Ishii T, Nagai T, Shiina M, Ogata K, Ohta T, Niikawa N, Miyatake S, Okada I, Mizuguchi T, Doi H, Saitsu H, Miyake N, Matsumoto N
  • Journal Title: Nat Genet Volume: 44 Issue: 4 Pages: 376-378
  • DOI: 10.1038/ng.2219
  • Peer Reviewed
• [Journal Article] Missense mutations in the DNA-binding/dimerization domain of NFIX cause Sotos-like features (2012)
  • Author(s): Yoneda Y, Saitsu H, Touyama M, Makita Y, Miyamoto A, Hamada K, Kurotaki N, Tomita H, Nishiyama K, Tsurusaki Y, Doi H, Miyake N, Ogata K, Naritomi K, Matsumoto N
  • Journal Title: J Hum Genet Volume: 57巻 Issue: 3 Pages: 207-211
  • DOI: 10.1038/jhg.2012.7
  • NAID: 10030712151
  • Peer Reviewed
• [Presentation] Search for anti-leukemic drugs targeting the transcription factor Runx1 by INTENDD (2017)
  • Author(s): Shiina M, Baba S, Uchiyama A, Okada C, Tanaka T, Ikeda K, Kawakita S, Matsuzaki T, Komatsu H, Hosoda M, Ogata K
  • Organizer: The 137th Annual Meeting of the Pharmaceutical Society of Japan, 3rd International Symposium for Medicinal Sciences
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2017-03-24
  • Int'l Joint Research / Invited
• [Presentation] パートナー因子によるEts1のコンフォーメーション分布の変化と活性制御機構の解析 (2016)
  • Author(s): 内山晃子，椎名政昭，浜田恵輔，馬場しほ，岡田千佳子，鈴木香絵，佐藤 光，緒方一博
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 転写因子Ets1の天然変性領域のリン酸化によるDNA結合制御機構の速度論的解析 (2016)
  • Author(s): 椎名政昭，笠原浩太，肥後順一，馬場しほ，内山晃子，岡田千佳子，浜田恵輔，佐藤 光，中村春木，緒方一博
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] Molecular behavior of a higher-order complex of multiple transcription factors on enhancer site upon phosphorylation (2016)
  • Author(s): Ogata K, Shiina M, Kasahara K, Higo J, Hamada K, Nakamura H
  • Organizer: 6th International Conference on Structural Biology (Structural Biology 2016)
  • Place of Presentation: New Orleans (Hilton New Orleans Airport Hotel), USA
  • Year and Date: 2016-08-22
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanism for modulation of a multiple transcription factor complex formed on enhancer site up on phosphorylation (2016)
  • Author(s): Ogata K, Shiina M, Kasahara K, Higo J, Hamada K, Nakamura H
  • Organizer: 5th International Conference and Exhibition on Metabolomics
  • Place of Presentation: Hyatt Regency Osaka（大阪府大阪市）
  • Year and Date: 2016-05-16
  • Int'l Joint Research / Invited
• [Presentation] Ets1とRunx1のエンハンサー上での立体的な位置関係と協調性 (2015)
  • Author(s): 内山晃子，椎名政昭，浜田恵輔，豊後泰子，嶋村麻利子，緒方一博
  • Organizer: BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市中央区）
  • Year and Date: 2015-12-01
• [Presentation] Kinetic characterization of a DNA binding activity of the transcription factor Ets1 and its regulation by phosphorylation in an intrinsically disordered region (2015)
  • Author(s): Shiina M, Kasahara K, Higo J, Baba S, Hamada K, Uchiyama A, Suzuki K, Sato K, Nakamura H, Ogata K
  • Organizer: BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市中央区）
  • Year and Date: 2015-12-01
• [Presentation] 分子構造に基づく転写因子Nuclear factor (erythroid-derived2)-like2(Nrf2)の抗酸化剤応答配列認識機構の解析 (2015)
  • Author(s): 鈴木香絵，椎名政昭，浜田恵輔，石川涼平，金井達哉，豊後泰子，岡田千佳子，馬場しほ，鈴木隆史，太田 力，山本雅之，緒方一博
  • Organizer: BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市中央区）
  • Year and Date: 2015-12-01
• [Presentation] Kinetic characterization of a DNA binding activity of the transcription factor Ets1 and its regulation by phosphorylation at an intrinsically disordered region (2014)
  • Author(s): Shiina M, Hamada K, Bungo-Inoue T, Shimamura M, Uchiyama A, Baba S, Sato K, Suzuki K, Yamamoto M, Ogata K
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県・横浜市)
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Orientation of Ets1 and Runx1 binding sites on enhancers and their cooperative regulation (2014)
  • Author(s): Uchiyama A, Shiina M, Hamada K, Bungo T, Shimamura M, Ogata K
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県・横浜市)
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Structure-based analysis for DNA recognition mechanism of the Nrf2-containing transcription factor heterodimer (2014)
  • Author(s): Suzuki K, Shiina M, Hamada K, Bungo T, Okada C, Baba S, Ohta T, Ogata K
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県・横浜市)
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] ヒストンシャペロンNap1の構造解析 (2014)
  • Author(s): 浜田恵輔，椎名政昭，中島理沙，斎藤 誠，伊藤 敬，緒方一博
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都府・京都市）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Regulation of DNA binding of Ets1 by phosphorylation in an intrinsic disordered region (2014)
  • Author(s): 椎名政昭，浜田恵輔，井上－豊後泰子，嶋村麻利子，馬場しほ，内山晃子，鈴木香絵，タヒロフ タヒール，緒方一博
  • Organizer: 第14回日本蛋白質科学会年会
  • Place of Presentation: ワークピア横浜（神奈川県・横浜市）
  • Year and Date: 2014-06-25 – 2014-06-27
  • Invited
• [Presentation] Crystal structural analysis of drosophila nucleosome assembly protein (2013)
  • Author(s): Hamada K, Shiina M, Nakashima R, Saito M, Ito T, Ogata K
  • Organizer: 4th International Symposium on Diffraction Structural Biology
  • Place of Presentation: 名古屋市中小企業振興会館（愛知県名古屋市）
• [Presentation] DNA-mediated allosteric regulation in a complex of multiple transcription factors with DNA (2013)
  • Author(s): Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Uchiyama A, Baba S, Sato K, Ogata K
  • Organizer: 4th International Symposium on Diffraction Structural Biology
  • Place of Presentation: 名古屋市中小企業振興会館（愛知県名古屋市）
• [Presentation] Structural analyses for the effects of Ets1 phosphorylation on Ets1-containing TF-DNA assemblies (2013)
  • Author(s): Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Uchiyama A, Baba S, Sato K, Yamamoto M, Tahirov TH, Ogata K
  • Organizer: HUPO 2013（12th Human Proteome Organization Annual World Congress）
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
• [Presentation] Cooperative Formation of a Multiple Transcription Factor Assembly on an Enhancer DNA and Its Regulation by Phosphorylation of a Transcription Factor via a Cell Signaling (2013)
  • Author(s): Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Uchiyama A, Baba S, Sato K, Ogata K
  • Organizer: HUPO 2013（12th Human Proteome Organization Annual World Congress）
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
• [Presentation] Allosteric Regulation of a Multiple Transcription Factor Assembly on an Enhancer DNA (2013)
  • Author(s): Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Uchiyama A, Baba S, Ko Sato K, Yamamoto M, Ogata K
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] Mechanisms of DNA recognition by the Nrf2-containing transcription factor heterodimer (2013)
  • Author(s): Suzuki K, Shiina M, Hamada K, Bungo T, Baba S, Ohta T, Ogata K
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] The cooperative activity of Ets1 and Runx1 on various target gene enhancers and the effect of phosphorylation of Ets1 on the cooperation (2013)
  • Author(s): Uchiyama A, Shiina M, Hamada K, Bungo T, Shimamura M, Ogata K
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
• [Presentation] Structural basis of DNA recognition by the Nrf2-containing heterodimer (2012)
  • Author(s): 鈴木香絵
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
  • Year and Date: 2012-12-13
• [Presentation] DNA-mediated allosteric regulation in a complex of multiple transcription factors with DNA (2012)
  • Author(s): 椎名政昭
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
  • Year and Date: 2012-12-12
• [Presentation] The correlation between some highly conserved residues of Ets family and cooperative DNA binding with Runx1 on various enhancers (2012)
  • Author(s): 内山晃子
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
  • Year and Date: 2012-12-12
• [Book] 見てわかる構造生命科学 生命科学研究へのタンパク質構造の利用 中村春木編 (2014)
  • Author(s): 椎名政昭，緒方一博
  • Total Pages: 28
  • Publisher: 化学同人
• [Book] 見てわかる構造生命科学;生命科学研究へのタンパク質構造の利用 中村春木編 (2014)
  • Author(s): 椎名政昭、緒方一博
  • Total Pages: 320
  • Publisher: 化学同人
• [Remarks] 横浜市立大学大学院医学研究科生化学教室ホームページ
  • URL: http://www-user.yokohama-cu.ac.jp/~seika/index.html
• [Remarks] 横浜市立大学大学院医学研究科
  • URL: http://www-user.yokohama-cu.ac.jp/~seika/index.html
• [Remarks] 横浜市立大学大学院医学研究科生化学ホームページ
  • URL: http://www-user.yokohama-cu.ac.jp/~seika/index.html
• [Remarks] 転写サイクルホームページ
  • URL: http://transcriptioncycle.org