| Budget Amount *help |
¥92,430,000 (Direct Cost: ¥71,100,000、Indirect Cost: ¥21,330,000)
Fiscal Year 2017: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2016: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2015: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2014: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2013: ¥29,120,000 (Direct Cost: ¥22,400,000、Indirect Cost: ¥6,720,000)
|
|---|
| Outline of Final Research Achievements |
We have developed a new analytical method that would enable us to perform structure and interaction analyses on multi-component equilibrium systems. Here, we have successfully developed the microfluidics-based, auto sampling equipment designed for continuous titration SAXS. Using this newly designed equipment, we can automatically collect numerous scattering profiles while altering the molar ratios of each component involved in the multi-component equilibrium; thus, enabling us to determine the system’s free energy landscape of the multi-component equilibrium. We performed a feasibility study of titration-SAXS measurements on a signal transduction system, which allow us to extract scattering curves of multiple complexes and their concentration dependence. The method is found to be applicable in wide range of understanding the dynamical ordering of complex molecular systems.
|
