| Budget Amount *help |
¥93,340,000 (Direct Cost: ¥71,800,000、Indirect Cost: ¥21,540,000)
Fiscal Year 2017: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2016: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
|
|---|
| Outline of Final Research Achievements |
In this study, several attempts were made to detect and control the dynamic ordering of proteins by site-specific introduction of nonnatural amino acids and nonnatural molecules. Throughout this study, unique methods were developed for expression of fluorescent antibodies that can detect antigen-binding as fluorescence change, in vivo expression of proteins containing nonnatural amino acids, molecular evolution of non-natural aminoacyl-tRNA synthetase within liposomes, specific chemical modification of nonnatural amino acid-containing proteins, introduction of nonnatural molecules into proteins, and inter- and intramolecular photocrosslinking of protein-protein interaction and protein conformational change. These achievements based on the incorporation of nonnatural molecules allowed detection and control of the dynamic ordering of proteins in vitro and in vivo.
|
