| Budget Amount *help |
¥56,680,000 (Direct Cost: ¥43,600,000、Indirect Cost: ¥13,080,000)
Fiscal Year 2017: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2016: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2015: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2014: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
|
|---|
| Outline of Final Research Achievements |
We developed a new method of single molecule fluorescence detection that can track single-molecule fluorescence and FRET efficiency at the time resolution of 10 microsecond. We used the method for the investigation of the folding dynamics of proteins, and revealed the unexpected heterogeneity in the unfolded state of BdpA and ubiquitin. In addition, we constructed a fluorescence microscope that can image the sliding of a tumor suppressor p53 along the stretched DNA, and revealed its target search dynamics. We clarified various properties of p53 including the dependency of the target search on the divalent cations, the behavior of p53 near the target sequence and the roles of disordered linker in p53. The current results might open a new research field of investigating proteins based on the assimilation of data obtained by single molecule method and MD calculation. In addition, investigations of the dynamics of proteins in the environment mimicking nucleus might become possible.
|
