| Budget Amount *help |
¥140,920,000 (Direct Cost: ¥108,400,000、Indirect Cost: ¥32,520,000)
Fiscal Year 2017: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2016: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2015: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2014: ¥36,530,000 (Direct Cost: ¥28,100,000、Indirect Cost: ¥8,430,000)
Fiscal Year 2013: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
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| Outline of Final Research Achievements |
We found that the TGF-b signaling system, especially the Nodal/activin-SMAD2 pathway, works upstream of Nanos2, an RNA binding protein essential for germ cell male differentiation. On the other hand, we found that the BMP-SMAD4 pathway is important for female differentiation. We showed that removal of two factors, Stra8 and SMAD4 in female germ cells resulted in induction of prospermatogonia, even in the ovary. Based on this finding, we proposed that an unexpected model that testis-specific inducers are not essential for the determination of maleness in germ cells. We demonstrated that RNA binding protein DND1, a partner protein of Nanos2, is essential for recognition and binding of Nanos2 target RNA.
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