| Budget Amount *help |
¥201,500,000 (Direct Cost: ¥155,000,000、Indirect Cost: ¥46,500,000)
Fiscal Year 2017: ¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2016: ¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2015: ¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2014: ¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2013: ¥33,540,000 (Direct Cost: ¥25,800,000、Indirect Cost: ¥7,740,000)
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| Outline of Final Research Achievements |
In 2004, we discovered that cultured spermatogonial stem cells, designated as germline stem (GS) cells, can spontaneously dedifferentiate into embryonic stem (ES) cell-like multipotent GS cells. However, the frequency of conversion was very low and the mechanism underlying this dedifferentiation remained unknown. In this research, we found that DNA demethylation in GS cells induces downregulation of Dmrt1, which is one of the germ cell tumor genes. This Dmrt1 downregulation leads to induce Sox2 gene, which is one of the essential genes involved in the maintenance of ES cells. Our results suggest that epigenetic abnormalities in GS cells are responsible for the acquisition of pluripotency in GS cells.
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