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Molecular mechanisms of small RNA-mediated epigenetic programming

Planned Research

Project AreaAnalyses and regulation of germline epigenome
Project/Area Number 25112005
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute of Genetics (2017)
Keio University (2013-2016)

Principal Investigator

SAITO KUNIAKI  国立遺伝学研究所, 系統生物研究センター, 教授 (30423396)

Project Period (FY) 2013-06-28 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥79,690,000 (Direct Cost: ¥61,300,000、Indirect Cost: ¥18,390,000)
Fiscal Year 2017: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2016: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2015: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2013: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
Keywordsエピゲノム / Piwi / piRNA / ショウジョウバエ / クロマチン / OSC / ヒストン / レトロトランスポゾン / 発現制御 / 生殖 / RNA / 遺伝子 / 蛋白質 / DmGTSF1
Outline of Final Research Achievements

In order to elucidate the function of Piwi, we looked for Piwi-interacting proteins and discovered a factor named DmGTSF1. We discovered that DmGTSF1 is essential for retrotransposon silencing and importance of Zn-finger motif of DmGTSF1. In addition, we found that linker histone H1 also interacts with Piwi. Biochemical analyses revealed that Piwi positively regulates the interaction between target retrotransposons and H1. Furthermore, ATAC-seq method enabled us to conclude that Piwi promotes chromatin aggregation of target retrotransposons via H1. Based on the above results, a molecular mechanism of epigenome regulation by small RNA was addressed.

Report

(6 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (19 results)

All 2017 2016 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 3 results,  Invited: 11 results)

  • [Journal Article] Piwi Modulates Chromatin Accessibility by Regulating Multiple Factors Including Histone H1 to Repress Transposons.2016

    • Author(s)
      Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K.
    • Journal Title

      Molecular Cell

      Volume: 63 Issue: 3 Pages: 408-419

    • DOI

      10.1016/j.molcel.2016.06.008

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian.2016

    • Author(s)
      Shibata N, Kashima M, Ishiko T, Nishimura O, Rouhana L, Misaki K, Yonemura S, Saito K, Siomi H, Siomi MC, Agata K.
    • Journal Title

      Developmental Cell

      Volume: 37 Issue: 3 Pages: 226-237

    • DOI

      10.1016/j.devcel.2016.04.009

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly.2014

    • Author(s)
      Murota Y, Ishizu H, Nakagawa S, Iwasaki YW, Shibata S, Kamatani MK, Saito K, Okano H, Siomi H, Siomi MC.
    • Journal Title

      Cell Rep.

      Volume: 8 Issue: 1 Pages: 103-113

    • DOI

      10.1016/j.celrep.2014.05.043

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] RNAi and overexpression of genes in ovarian somatic cells.2014

    • Author(s)
      Saito K.
    • Journal Title

      Methods in Molecular Biology

      Volume: 1093 Pages: 25-33

    • DOI

      10.1007/978-1-62703-694-8_3

    • ISBN
      9781627036931, 9781627036948
    • Related Report
      2013 Annual Research Report
  • [Journal Article] DmGTSF1 is necessary for Piwi-piRISC-mediated transcriptional transposon silencing in the Drosophila ovary.2013

    • Author(s)
      Ohtani H, Iwasaki YW, Shibuya A, Siomi H, Siomi MC, Saito K.
    • Journal Title

      Genes Dev.

      Volume: 27 Issue: 15 Pages: 1656-61

    • DOI

      10.1101/gad.221515.113

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] piRNA-mediated epigenetic control of transposable elements in Drosophila.2017

    • Author(s)
      Saito K.
    • Organizer
      The International Research Symposium on Regulation of Germ Cell Development in vivo and in vitro
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] piRNA-mediated chromatin regulation in Drosophila gonadal cells2017

    • Author(s)
      Saito K.
    • Organizer
      Symposium on Epigenome Dynamics and Regulation in Germ Cells
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] piRNAによるクロマチン制御の分子機構2017

    • Author(s)
      Saito K.
    • Organizer
      Hokuriku RNA Club
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Small RNA-induced epigenetic control in Drosophila2017

    • Author(s)
      Saito K.
    • Organizer
      ConBio 2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Piwi promotes transcriptional silencing by regulating chromatin compaction2016

    • Author(s)
      Saito K, Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H.
    • Organizer
      Keystone Symposia. Small RNA Silencing: Little Guides, Big Biology
    • Place of Presentation
      Colorado USA
    • Year and Date
      2016-01-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Piwi silences transposon transcription by regulating chromatin compaction.2016

    • Author(s)
      Saito K.
    • Organizer
      Cell Symposia: Functional RNAs
    • Place of Presentation
      中国(広州)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ショウジョウバエpiRNAによるクロマチン制御機構2016

    • Author(s)
      齋藤都暁
    • Organizer
      大阪大学蛋白質研究所セミナー“生命システムを支配するエピジェネティクス”
    • Place of Presentation
      蛋白質研究所(大阪府吹田市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Comparison of ovary- and testis-derived piRNAs reveals gender-specific piRNA clusters in Syrian hamster2015

    • Author(s)
      Saito K, Seki N, Tsuchiya M, Hirano T, Iwasaki YW, Siomi MC, Siomi H.
    • Organizer
      The Twentieth Annual Meeting of the RNA Society
    • Place of Presentation
      Madison USA
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ショウジョウバエPiwi-piRNA複合体によるクロマチン制御機構2015

    • Author(s)
      齋藤都暁
    • Organizer
      第9回日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京都千代田区)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Piwi-piRISC regulates binding of linker histone H1 at transposon loci2014

    • Author(s)
      Saito K.
    • Organizer
      Tokyo RNA Club
    • Place of Presentation
      東京
    • Year and Date
      2014-11-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Molecular mechanisms of piRNA-mediated transposon silencing2014

    • Author(s)
      Saito K.
    • Organizer
      日本遺伝学会年会
    • Place of Presentation
      長浜
    • Year and Date
      2014-09-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] DmGTSF1 is required for Piwi-piRISC-mediated transcriptional transposon silencing2013

    • Author(s)
      Saito K, Ohtani H, Iwasaki YW, Shibuya A, Siomi H, Siomi MC
    • Organizer
      日本RNA学会
    • Place of Presentation
      ひめぎんホール(愛媛県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ショウジョウバエのpiRNAによるトランスポゾン制御2013

    • Author(s)
      齋藤都暁
    • Organizer
      国立遺伝学研究所 研究集会「転移因子と宿主の相互作用による生命機能」
    • Place of Presentation
      国立遺伝学研究所(静岡県)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] piRNA pathway in Drosophila2013

    • Author(s)
      Saito K
    • Organizer
      Small RNAs to stem Cells & Epigenetics Reprogramming ASIA-2013 Meeting
    • Place of Presentation
      東京大学山上会館(東京都)
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2013-07-10   Modified: 2019-07-29  

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