| Budget Amount *help |
¥79,690,000 (Direct Cost: ¥61,300,000、Indirect Cost: ¥18,390,000)
Fiscal Year 2017: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2016: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2015: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2013: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
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| Outline of Final Research Achievements |
In this study, we examined the function of totipotent-cell specific genes in vivo. We found that Klf17 and Btg4 are maternal effect genes required for preimplantation development. We also revealed that Klf17 and Btg4 are involved in zygotic gene activation and maternal RNA degradation, respectively. We also found that Trim61 possess E3 ubiquitin ligase activity, and is maternal-zygotic effect gene required for preimplantation development. In additoin, we revealed that Pramef12 plays important role in differentiation of male primordial germ cells, and is required for spermatogenesis. Furthermore, we defined culture condition for ESCs that leads to increases the population of totipotent cells.
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