Functional analysis of fertilization related genes in mice

• Project Area: Analyses and regulation of germline epigenome
• Project/Area Number: 25112007
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: IKAWA MASAHITO 大阪大学, 微生物病研究所, 教授 (20304066)
• Co-Investigator(Renkei-kenkyūsha): ISOATANI Ayako 奈良先端科学技術大学院大学, 器官発生工学, 准教授 (20444523) ; SATOUH Yuhkoh 大阪大学, 微生物病研究所, 講師 (40545571) ; FUJIHARA Yoshitaka 大阪大学, 微生物病研究所, 助教 (70578848) ; MIYATA Haruhiko 大阪大学, 微生物病研究所, 助教 (50604732)
• Project Period (FY): 2013-06-28 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥169,000,000 (Direct Cost: ¥130,000,000、Indirect Cost: ¥39,000,000); Fiscal Year 2017: ¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000); Fiscal Year 2016: ¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000); Fiscal Year 2015: ¥32,370,000 (Direct Cost: ¥24,900,000、Indirect Cost: ¥7,470,000); Fiscal Year 2014: ¥32,370,000 (Direct Cost: ¥24,900,000、Indirect Cost: ¥7,470,000); Fiscal Year 2013: ¥48,620,000 (Direct Cost: ¥37,400,000、Indirect Cost: ¥11,220,000)
• Keywords: ゲノム編集 / 生殖不全 / 遺伝子破壊 / 点変異 / 受精 / 不妊 / 精子機能 / 卵子機能 / 胎盤 / 実験動物 / ノックイン / 精子 / 卵子 / CRISPR/Cas / ノックアウト / 精子形成 / 人工制限酵素 / 遺伝子組換え

Outline of Final Research Achievements

We applied CRISPR/Cas9 mediated genome editing in fertilized eggs and ES cells to study fertilization related genes in mice. While 54 of 80 testis-specific genes are not essential for male fertility, we found (1) sperm calcineurin is essential for sperm motility and could be male contraceptive target, (2) W62 of oocyte JUNO plays pivotal role to interact with sperm IZUMO1 and subsequent fertilization, and (3) sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice.

Research Products

• [Int'l Joint Research] Baylor College of Medecine/University of Hawaii(米国)
• [Int'l Joint Research] University of New Castle(オーストラリア)
• [Int'l Joint Research] ソウル大学(韓国)
• [Int'l Joint Research] Baylor College of Medicine(米国)
• [Int'l Joint Research] University of New Castle(オーストラリア)
• [Int'l Joint Research] ソウル大学(韓国)
• [Int'l Joint Research] Baylor College of Medicine(米国)
• [Int'l Joint Research] University of Cambridge(英国)
• [Int'l Joint Research] University of New Castle(Australia)
• [Int'l Joint Research] Seoul National University(韓国)
• [Journal Article] Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice (2018)
  • Author(s): Nozawa Kaori、Satouh Yuhkoh、Fujimoto Takao、Oji Asami、Ikawa Masahito
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1315-1315
  • DOI: 10.1038/s41598-018-19497-6
  • NAID: 120006957442
  • Peer Reviewed / Open Access
• [Journal Article] Factors controlling sperm migration through the oviduct revealed by gene-modified mouse models (2018)
  • Author(s): fujihara Y, Miyata H, Ikawa M.
  • Journal Title: Experimental Animals Volume: 67 Issue: 2 Pages: 91-104
  • DOI: 10.1538/expanim.17-0153
  • NAID: 130006733631
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] A delayed sperm penetration of cumulus layers by disruption of acrosin gene in rats. (2017)
  • Author(s): Isotani A, Matsumura T, Ogawa M, Tanaka T, Yamagata K, Ikawa M, Okabe M.
  • Journal Title: Biology of Reproduction Volume: 97(1) Issue: 1 Pages: 61-68
  • DOI: 10.1093/biolre/iox066
  • Peer Reviewed / Open Access
• [Journal Article] TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa. (2017)
  • Author(s): Castaneda JM, Hua R, Miyata H, Oji A, Guo Y, Cheng Y, Zhou T, Guo X, Cui Y, Shen B, Wang Z, Hu Z, Zhou Z, Sha J, Prunskaite-Hyyrylainen R , Yu Z, Ramirez-Solis R, Ikawa M, Matzuk MM and Liu M
  • Journal Title: Proc Natl Acad Sci U S A Volume: 114(27) Issue: 27
  • DOI: 10.1073/pnas.1621279114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR/Cas9. (2017)
  • Author(s): Abbasi F, Miyata H, Ikawa M.
  • Journal Title: Reproductive Medicine and Biology Volume: 17(1) Issue: 1 Pages: 3-10
  • DOI: 10.1002/rmb2.12067
  • Peer Reviewed / Open Access
• [Journal Article] Placenta-specific gene manipulation using lentiviral vector and its application (2017)
  • Author(s): Tobita Tomohiro、Kiyozumi Daiji、Ikawa Masahito
  • Journal Title: Placenta. Volume: 59 Pages: S37-S43
  • DOI: 10.1016/j.placenta.2017.09.012
  • Peer Reviewed / Open Access
• [Journal Article] Viable offspring after imaging of Ca2+ oscillations and visualization of the cortical reaction in mouse eggs. (2017)
  • Author(s): Satouh Y, Nozawa K, Yamagata K, Fujimoto T, Ikawa M.
  • Journal Title: Biol Reprod Volume: 96 Issue: 3 Pages: 563-575
  • DOI: 10.1093/biolre/iox002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Proton Pump Inhibitors Decrease Soluble fms-Like Tyrosine Kinase-1 and Soluble Endoglin Secretion, Decrease Hypertension, and Rescue Endothelial Dysfunction. (2017)
  • Author(s): Onda K, Tong S, Beard S, Binder N, Muto M, Senadheera SN, Parry L, Dilworth M, Renshall L, Brownfoot F, Hastie R, Tuohey L, Palmer K, Hirano T, Ikawa M, Kaitu'u-Lino T, Hannan NJ.
  • Journal Title: Hypertension Volume: 69 Issue: 3 Pages: 457-468
  • DOI: 10.1161/hypertensionaha.116.08408
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis (2017)
  • Author(s): Ueda J., Harada A., Urahama T., Machida S., Maehara K., Hada M., Makino Y., Nogami J., Horikoshi N., Osakabe A., Taguchi H., Tanaka H., Tachiwana H., Yao T., Yamada M., Iwamoto T., Isotani A., Ikawa M., Tachibana T., Okada Y., Kimura H., Ohkawa Y., Kurumizaka H., Yamagata K.
  • Journal Title: Cell Reports Volume: 18(3) Issue: 3 Pages: 593-600
  • DOI: 10.1016/j.celrep.2016.12.065
  • NAID: 120006957416
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] GPI-AP release in cellular, developmental, and reproductive biology. (2016)
  • Author(s): Fujihara Y, Ikawa M.
  • Journal Title: J Lipid Res. Volume: 57 Issue: 4 Pages: 538-545
  • DOI: 10.1194/jlr.r063032
  • NAID: 120006957424
  • Peer Reviewed / Open Access
• [Journal Article] CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa. (2016)
  • Author(s): Young SA, Miyata H, Satouh Y, Aitken RJ, Baker MA, Ikawa M.
  • Journal Title: J Cell Sci. Volume: 129 Issue: 23 Pages: 4379-4387
  • DOI: 10.1242/jcs.193151
  • NAID: 120006957405
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is dispensable for IZUMO1 function and male fertility. (2016)
  • Author(s): Young SA, Miyata H, Satouh Y, Muto M, Larsen MR, Aitken RJ, Baker M, Ikawa M.
  • Journal Title: Reproduction. Volume: 152 Issue: 6 Pages: 665-672
  • DOI: 10.1530/rep-16-0150
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice. (2016)
  • Author(s): Oji A, Noda T, Fujihara Y, Miyata H, Kim YJ, Muto M, Nozawa K, Matsumura T, Isotani A, Ikawa M.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 34666-34666
  • DOI: 10.1038/srep31666
  • NAID: 120006957419
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization. (2016)
  • Author(s): Kato K, Satouh Y, Nishimasu H, Kurabayashi A, Morita J, Fujihara Y, Oji A, Ishitani R, Ikawa M, Nureki O.
  • Journal Title: Nat Commun. Volume: 7 Issue: 1 Pages: 12198-12198
  • DOI: 10.1038/ncomms12059
  • NAID: 120006957420
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice. (2016)
  • Author(s): Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 113 Issue: 28 Pages: 7704-7710
  • DOI: 10.1073/pnas.1608458113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and it is essential for reproduction (2016)
  • Author(s): Daisuke Yamazaki, Haruhiko Miyata, Yosuke Funato, Yoshitaka Fujihara, Masahito Ikawa, Hiroaki Miki
  • Journal Title: Journal of Cell Science Volume: 未定 Issue: 9 Pages: 1940-1949
  • DOI: 10.1242/jcs.182220
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Generation of Hprt-disrupted rat through mouse←rat ES chimeras. (2016)
  • Author(s): Isotani A, Yamagata K, Okabe M, Ikawa M
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 24215-24215
  • DOI: 10.1038/srep24215
  • NAID: 120006957423
  • Peer Reviewed / Open Access
• [Journal Article] Behavior of Mouse Spermatozoa in the Female Reproductive Tract from Soon after Mating to the Beginning of Fertilization. (2016)
  • Author(s): Muro Y, Hasuwa H, Isotani A, Miyata H, Yamagata K, Ikawa M, Yanagimachi R, Okabe M
  • Journal Title: Biol Reprod Volume: 印刷中 Issue: 4 Pages: 80-80
  • DOI: 10.1095/biolreprod.115.135368
  • Peer Reviewed / Open Access
• [Journal Article] Lentiviral Vector Mediated Complementation Restored Fetal Viability but Not Placental hyperplasia in Plac1-Deficient Mice. (2016)
  • Author(s): Muto M, Fujihara Y, Tobita T, Kiyozumi D, Ikawa M.
  • Journal Title: Biol Reprod. Volume: 94(1) Issue: 1 Pages: 6-6
  • DOI: 10.1095/biolreprod.115.133454
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks. (2015)
  • Author(s): Latos PA, Sienerth AR, Murray A, Senner CE, Muto M, Ikawa M, Oxley D, Burge S, Cox BJ, Hemberger M.
  • Journal Title: Genes Dev. Volume: 29 Issue: 23 Pages: 2435-48
  • DOI: 10.1101/gad.268821.115
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive (2015)
  • Author(s): Haruhiko Miyata, Yuhkoh Satouh, Daisuke Mashiko, Masanaga Muto, Kaori Nozawa, Kogiku Shiba, Yoshitaka Fujihara, Ayako Isotani, Kazuo Inaba, Masahito Ikawa
  • Journal Title: Science Volume: 350 Issue: 6259 Pages: 442-445
  • DOI: 10.1126/science.aad0836
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis (2015)
  • Author(s): Samantha AM Young, Haruhiko Miyata, Yuhkoh Satouh, Hirotaka Kato, Kaori Nozawa, Ayako Isotani, R John Aitken, Mark A Baker, Masahito Ikawa
  • Journal Title: Int. J. Mol. Sci Volume: 16 Issue: 10 Pages: 24732-24750
  • DOI: 10.3390/ijms161024732
  • NAID: 120006957408
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Sperm postacrosomal WW domain-binding protein is not required for mouse egg activation. (2015)
  • Author(s): Satouh Y, Nozawa K, Ikawa M
  • Journal Title: Biol Reprod. Volume: 93 Issue: 4 Pages: 94-94
  • DOI: 10.1095/biolreprod.115.131441
  • Peer Reviewed
• [Journal Article] Calreticulin is required for development of the cumulus oocyte complex and female fertility. (2015)
  • Author(s): Tokuhiro K, Satouh Y, Nozawa K, Isotani A, Fujihara Y, Hirashima Y, Matsumura H, Takumi K, Miyano T, Okabe M, Benham AM, Ikawa M.
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 14254-14254
  • DOI: 10.1038/srep14254
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A novel transcriptional factor nkapl is a germ cell-specific suppressor of notch signaling and is indispensable for spermatogenesis. (2015)
  • Author(s): Okuda, H., Kiuchi, H., Takao, T., Miyagawa, Y., Tsujimura, A., Nonomura, N., Miyata, H., Okabe, M., Ikawa, M., Kawakami, Y., et al.
  • Journal Title: PLoS ONE Volume: 4 Issue: 4 Pages: e0124293-e0124293
  • DOI: 10.1371/journal.pone.0124293
  • NAID: 120006957411
  • Peer Reviewed / Open Access
• [Journal Article] Pluripotent stem cells derived from mouse primordial germ cells by small molecule compounds. (2015)
  • Author(s): Kimura T et al.
  • Journal Title: Stem Cells Volume: 33 Issue: 1 Pages: 45-55
  • DOI: 10.1002/stem.1838
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] NUCKS Is a Positive Transcriptional Regulator of Insulin Signaling. (2014)
  • Author(s): Qiu B, Shi X, Wong ET, Lim J, Bezzi M, Low D, Zhou Q, Akincilar SC, Lakshmanan M, Swa HL, Tham JM, Gunaratne J, Cheng KK, Hong W, Lam KS, Ikawa M, Guccione E, Xu A, Han W, Tergaonkar V.
  • Journal Title: Cell Rep. Volume: 7 Issue: 6 Pages: 1876-86
  • DOI: 10.1016/j.celrep.2014.05.030
  • Peer Reviewed / Open Access
• [Journal Article] Effect of isolated AMP deaminase deficiency on skeletal muscle function. (2014)
  • Author(s): Chenga j, Morisaki H, Sugimoto N, Dohi A, Shintani T, Kimura E, Toyama K, Ikawa M, Okabe M, Higuchi I, Matsuo S, Kawai Y, Hisatome I, Sugama T, Holmes E. W. , *Morisaki T.
  • Journal Title: Molecular Genetics and Metabolism Reports Volume: 1 Pages: 51-9
  • DOI: 10.1016/j.ymgmr.2013.12.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. (2014)
  • Author(s): Mizuno S, Dinh TT, Kato K, Mizuno-Iijima S, Tanimoto Y, Daitoku Y, Hoshino Y, Ikawa M, Takahashi S, Sugiyama F, Yagami KI.
  • Journal Title: Mamm Genome. Volume: 25 Issue: 7-8 Pages: 327-34
  • DOI: 10.1007/s00335-014-9524-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Deletion of N-myc downstream-regulated gene 2 attenuates reactive astrogliosis and inflammatory response in a mouse model of cortical stab injury. (2014)
  • Author(s): Takarada-Iemata M, Kezuka D, Takeichi T, Ikawa M, Hattori T, Kitao Y, Hori O.
  • Journal Title: J Neurochem. Volume: 印刷中 Issue: 3 Pages: 374-87
  • DOI: 10.1111/jnc.12729
  • NAID: 120005478796
  • Peer Reviewed
• [Journal Article] Genome Editing in Mice Using CRISPR/Cas (2014)
  • Author(s): Young S, Baker M, Ikawa M.
  • Journal Title: Targeted Genome Editing Using Site-Specific Nucleases Volume: － Pages: 151-166
  • Peer Reviewed
• [Journal Article] CRISPR/Cas9-Based Genome Editing in Mice by Single Plasmid Injection (2014)
  • Author(s): Fujihara Y, Ikawa M.
  • Journal Title: Methods Enzymol Volume: 546C Pages: 319-336
  • Peer Reviewed
• [Journal Article] GPI-Anchored Protein Complex, LY6K/TEX101, Is Required for Sperm Migration into the Oviduct and Male Fertility in Mice. (2014)
  • Author(s): Fujihara, Y., Okabe, M., and Ikawa, M.
  • Journal Title: Biology of Reproduction Volume: 90 Issue: 3 Pages: 1-6
  • DOI: 10.1095/biolreprod.113.112888
  • Peer Reviewed
• [Journal Article] Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes (2014)
  • Author(s): Mashiko D, Young SA, Muto M, Kato H, Nozawa K, Ogawa M, Noda T, Kim YJ, Satouh Y, Fujihara Y, Ikawa M.
  • Journal Title: Development, Growth & Differentiation Volume: 56 Issue: 1 Pages: 122-129
  • DOI: 10.1111/dgd.12113
  • Peer Reviewed
• [Journal Article] Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. (2013)
  • Author(s): Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M.
  • Journal Title: Scientific Reports Volume: 3: 3355 Issue: 1 Pages: 1-6
  • DOI: 10.1038/srep03355
  • NAID: 120006957396
  • Peer Reviewed
• [Journal Article] MiR-200b and miR-429 Function in Mouse Ovulation and Are Essential for Female Fertility. (2013)
  • Author(s): Hasuwa H, Ueda J, Ikawa M, Okabe M.
  • Journal Title: Science. Volume: 341 Issue: 6141 Pages: 71-3
  • DOI: 10.1126/science.1237999
  • Peer Reviewed
• [Journal Article] Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa (2013)
  • Author(s): FujiharaY, Tokuhiro K, Muro Y, Kondoh G, Araki Y, Ikawa M, Okabe M
  • Journal Title: PNAS Volume: 110(20) Issue: 20 Pages: 8111-6
  • DOI: 10.1073/pnas.1222166110
  • Peer Reviewed
• [Presentation] CRISPR/Cas9 mediated genome editing in mice and its application for the study of reproduction (2018)
  • Author(s): 伊川 正人
  • Organizer: 第9回武田科学振興財団薬科学シンポジウム
  • Invited
• [Presentation] CRISPR/Cas9 mediated genome editing in mice and its application for the study of reproduction (2018)
  • Author(s): 伊川 正人
  • Organizer: Bridging Biomedical Worlds 2018
  • Int'l Joint Research / Invited
• [Presentation] Lentiviral vector-mediated placenta-specific gene manipulation in mice. (2017)
  • Author(s): 伊川正人
  • Organizer: 64th SRI meeting
  • Place of Presentation: Hilton Orlando Bonnet Creek(Orlando, USA)
  • Year and Date: 2017-03-15
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集マウス作製と受精研究への応⽤ (2017)
  • Author(s): 伊川 正人
  • Organizer: 第40回日本分子生物学会年会
  • Invited
• [Presentation] ゲノム編集技術の進歩と課題 (2017)
  • Author(s): 伊川 正人
  • Organizer: 日本動物実験代替法学会
  • Invited
• [Presentation] ゲノム編集を利用した精巣特異的遺伝子の網羅的機能解析 (2017)
  • Author(s): 伊川 正人
  • Organizer: 第3回生体調節研究所 内分泌代謝シンポジウム
  • Invited
• [Presentation] CRISPR/Cas9 mediated genome editing and its application for the study of reproduction (2017)
  • Author(s): 伊川 正人
  • Organizer: 4th World Congress of Reproductive Biology (WCRB 2017)
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集による遺伝子改変マウス作製とその応用 (2017)
  • Author(s): 伊川 正人
  • Organizer: 第２回秋期特別日本血管生物医学会シンポジウム
  • Invited
• [Presentation] Fertilization and Oocyte Activation: New Insights Using Genome Editing (2017)
  • Author(s): 伊川 正人
  • Organizer: Fertilization & Activation of Development(Gordon Research Conference)
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9 Mediated Genome Editing and its Application for the Study of Reproduction (2017)
  • Author(s): 伊川 正人
  • Organizer: JST戦略的国際共同研究プログラム
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9 mediated genome editing and its application for the study of sperm functions (2017)
  • Author(s): 伊川 正人
  • Organizer: 11th International Congress of Andrology
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集を用いた受精関連遺伝子の網羅的機能解析 (2016)
  • Author(s): 伊川正人
  • Organizer: 新学術領域研究「生殖細胞のエピゲノムダイナミクスとその制御」第４回公開シンポジウム
  • Place of Presentation: 三島市民文化会館(静岡県三島市)
  • Year and Date: 2016-11-17
  • Invited
• [Presentation] CRISPR/Cas9 mediated genome editing and its application for the study of reproduction (2016)
  • Author(s): 伊川正人
  • Organizer: 2016 Seoul Symposium on Epigenetics and Chromatin
  • Place of Presentation: ソウル大学(Seoul, Korea)
  • Year and Date: 2016-11-09
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9 mediated genome editing and its application for the study of reproduction. (2016)
  • Author(s): 伊川正人
  • Organizer: The 4th SKLRB Symposium on Reproductive Biology
  • Place of Presentation: UCASinternational Conference Center(Beijing, China)
  • Year and Date: 2016-10-27
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9 mediated genome editing and its application for the study of reproduction (2016)
  • Author(s): 伊川 正人
  • Organizer: 国際シンポジウム "生殖細胞のエピゲノムダイナミクスとその制御"
  • Place of Presentation: 京都大学百周年時計台記念館 （京都府京都市）
  • Year and Date: 2016-02-17
  • Int'l Joint Research
• [Presentation] CRISPR/Cas9 mediated genome editing in mice (2016)
  • Author(s): 伊川 正人
  • Organizer: Lecture Series from Osaka University, Japan
  • Place of Presentation: バンドン工科大学（ITB)（インドネシア・バンドン）
  • Year and Date: 2016-01-21
  • Invited
• [Presentation] CRISPR/Cas-Mediated Genome Editing in Mice and Its Application for the Study of Reproduction (2015)
  • Author(s): 伊川 正人
  • Organizer: 第48回米国生殖学会会議（SSR 2015 Annual Meeting）
  • Place of Presentation: Puerto Rico Convention Center（プエルトリコ・サンファン）
  • Year and Date: 2015-06-18
  • Int'l Joint Research / Invited
• [Presentation] 遺伝子改変動物が導く受精メカニズムの新論争 (2015)
  • Author(s): 伊川 正人
  • Organizer: 第60回日本生殖医学会学術講演会・IFFS／JSRM International Meeting 2015
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2015-04-26
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Casシステムが開く遺伝子改変マウスの未来 (2015)
  • Author(s): 伊川正人
  • Organizer: 第86回東海実験動物研究会
  • Place of Presentation: 岐阜大学（岐阜市）
  • Year and Date: 2015-03-28
  • Invited
• [Presentation] ADAM3と精子受精能力 (2015)
  • Author(s): 伊川正人
  • Organizer: 第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会合同大会
  • Place of Presentation: 神戸国際会議場（神戸市）
  • Year and Date: 2015-03-21
  • Invited
• [Presentation] CRISPR/Cas Mediated Genome Editing in Mice and its Application for the Study of Reproduction (2015)
  • Author(s): 伊川正人
  • Organizer: BRI International Symposium 2015
  • Place of Presentation: 新潟大学脳研究所（新潟市）
  • Year and Date: 2015-03-05
  • Invited
• [Presentation] CRISPR/Casを用いたマウスゲノム編集 (2014)
  • Author(s): 伊川正人
  • Organizer: 国立大学法人動物実験施設協議会平成26年度（第20回）高度技術研修会
  • Place of Presentation: 新潟大学脳研究所生命科学リソース研究センター（新潟市）
  • Year and Date: 2014-12-04
  • Invited
• [Presentation] CRISPR/Casシステムが開く遺伝子改変マウスの未来 (2014)
  • Author(s): 伊川正人
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-11-25
  • Invited
• [Presentation] CRISPR/CAS mediated genome editing in mice (2014)
  • Author(s): 伊川正人
  • Organizer: The 27th Annual Meeting of Japanese Association for Animal Cell Technology, JAACT2014 Kitakyushu
  • Place of Presentation: Kitakyushu International Conference Center（北九州市）
  • Year and Date: 2014-11-14
  • Invited
• [Presentation] CRISPR/Cas システムを用いたマウスゲノム編集 (2014)
  • Author(s): 伊川正人
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: パシフィコ横浜会議センター（横浜市）
  • Year and Date: 2014-11-12
  • Invited
• [Presentation] ゲノム編集を用いた精子受精能関連因子の機能解析 (2014)
  • Author(s): 伊川正人
  • Organizer: 新学術領域研究「生殖細胞のエピゲノムダイナミクスとその制御」第２回公開シンポジウム
  • Place of Presentation: 九州大学・病院キャンパス（福岡市）
  • Year and Date: 2014-11-01
• [Presentation] CRISPR/Casシステムを用いたマウスゲノム編集 (2014)
  • Author(s): 伊川正人
  • Organizer: 第4回ゲノム編集研究会
  • Place of Presentation: 広島国際会議場（広島市）
  • Year and Date: 2014-10-06
• [Presentation] CRISPR/Casシステムを用いたマウスゲノム編集 (2014)
  • Author(s): 伊川正人
  • Organizer: 第1回 KBRP ワークショップ
  • Place of Presentation: 熊本大学生命資源研究・支援センター（熊本市）
  • Year and Date: 2014-10-03
  • Invited
• [Presentation] CRISPR/Casを用いたマウスゲノム編集と表現型解析 (2014)
  • Author(s): 伊川正人
  • Organizer: がん支援活動公開シンポジウム
  • Place of Presentation: 一橋講堂（東京都千代田区）
  • Year and Date: 2014-08-21
  • Invited
• [Presentation] CRISPR/Cas mediated genome editing in mice and its application for reproductive studies (2014)
  • Author(s): 伊川正人
  • Organizer: The 12th International Symposium on Spermatology
  • Place of Presentation: Newcastle City Hall（Newcastle, Australia）
  • Year and Date: 2014-08-14
  • Invited
• [Presentation] CRISPR/CASシステムを用いたマウスゲノム編集 (2014)
  • Author(s): 伊川正人
  • Organizer: 第41回日本毒性学会学術年会
  • Place of Presentation: 神戸コンベンションセンター（神戸市）
  • Year and Date: 2014-07-04
  • Invited
• [Presentation] CRISPR/CAS mediated genome editing in mice (2014)
  • Author(s): 伊川正人
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂（奈良市）
  • Year and Date: 2014-06-11
  • Invited
• [Presentation] CRISPR/Cas人工制限酵素システムを用いたマウスゲノム編集 (2014)
  • Author(s): 伊川正人
  • Organizer: ワークショップ「ゲノム編集の現状と可能性」
  • Place of Presentation: 九州大学（福岡市）
  • Year and Date: 2014-05-23
  • Invited
• [Presentation] Question about the necessity of sperm binding to zona pellucida
  • Author(s): 伊川 正人
  • Organizer: SSR2013
  • Place of Presentation: Palais des congrès de Montréal （Canada）
  • Invited
• [Presentation] CRISPR/CASシステムを用いたマウスゲノム編集
  • Author(s): 伊川 正人
  • Organizer: 第36回分子生物学会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Invited
• [Presentation] CRISPR/CASシステムを用いたマウスゲノム編集
  • Author(s): 伊川 正人
  • Organizer: 第３回ゲノム編集研究会
  • Place of Presentation: 広島大学理学部（広島県東広島市）
  • Invited
• [Book] マウスでのゲノム編集 実験医学増刊 Vol.34 No.20 All Aboutゲノム編集 (2016)
  • Author(s): 野田大地，大字亜沙美，伊川正人
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] ゲノム編集技術を使った遺伝子改変マウスの作製 実験医学別冊 マウス表現型解析スタンダード (2016)
  • Author(s): 野田大地，大字亜沙美，伊川正人
  • Total Pages: 9
  • Publisher: 羊土社
• [Remarks] 大阪大学教員基本
  • URL: http://www.dma.jim.osaka-u.ac.jp/view?l=ja&u=867
• [Remarks] 研究室HP
  • URL: http://www.egr.biken.osaka-u.ac.jp/
• [Funded Workshop] 国際シンポジウム "生殖細胞のエピゲノムダイナミクスとその制御" (2016)
  • Place of Presentation: 京都大学百周年時計台記念館 （京都府京都市）
  • Year and Date: 2016-02-17