| Budget Amount *help |
¥152,230,000 (Direct Cost: ¥117,100,000、Indirect Cost: ¥35,130,000)
Fiscal Year 2017: ¥36,530,000 (Direct Cost: ¥28,100,000、Indirect Cost: ¥8,430,000)
Fiscal Year 2016: ¥36,530,000 (Direct Cost: ¥28,100,000、Indirect Cost: ¥8,430,000)
Fiscal Year 2015: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
Fiscal Year 2014: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000)
Fiscal Year 2013: ¥33,540,000 (Direct Cost: ¥25,800,000、Indirect Cost: ¥7,740,000)
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| Outline of Final Research Achievements |
Epigenetic changes during the germline cycle were analyzed by nuclear transfer techniques using somatic cells and other cell types as donors. We found that somatic, repressive histone marks such as H3K9me2 and me3 were resistant to reprogramming by nuclear transfer and caused developmental failure of somatically cloned embryos. Another histone mark, H3K27me3, which controls placenta-specific imprinting was lost in cloned embryos, leading to placental enlargement. A histone arginine methylation mark, H3R17me2, was essential for incorporation of maternal H3.3 into the paternal genome and active DNA demethylation of the paternal genome. Thus, we uncovered the importance of histone methylation during the germline cycle.
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